Detailed view for Rv0636

Basic information

TDR Targets ID: 7495
Mycobacterium tuberculosis, (3R)-hydroxyacyl-ACP dehydratase subunit HadB
Source Database / ID:  Tuberculist 

pI: 6.4691 | Length (AA): 142 | MW (Da): 14934 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01575   MaoC like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 142 4rlj (B) 1 142 99.99 0 1 2.2181 -1.28

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 4RLJ:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4RLT:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4RLU:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4RLW:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase. hasan
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_130995)

Species Accession Gene Product
Dictyostelium discoideum DDB_G0281965   hypothetical protein
Leishmania braziliensis LbrM.07.0450   3-hydroxyacyl-ACP dehydratase, putative
Leishmania braziliensis LbrM.07.0460   3-hydroxyacyl-ACP dehydratase, putative
Leishmania donovani LdBPK_070180.1   maoc family dehydratase-like protein
Leishmania donovani LdBPK_070170.1   maoc family dehydratase-like protein
Leishmania infantum LinJ.07.0170   3-hydroxyacyl-ACP dehydratase, putative
Leishmania infantum LinJ.07.0180   3-hydroxyacyl-ACP dehydratase, putative
Leishmania major LmjF.07.0440   3-hydroxyacyl-ACP dehydratase, putative
Leishmania major LmjF.07.0430   3-hydroxyacyl-ACP dehydratase, putative
Leishmania mexicana LmxM.07.0430   maoc family dehydratase-like protein
Leishmania mexicana LmxM.07.0440   maoc family dehydratase-like protein
Mycobacterium leprae ML1909c   conserved hypothetical protein
Mycobacterium tuberculosis Rv0636   (3R)-hydroxyacyl-ACP dehydratase subunit HadB
Mycobacterium ulcerans MUL_0721   (3R)-hydroxyacyl-ACP dehydratase subunit HadB
Schistosoma japonicum Sjp_0085530   ko:K01164 ribonuclease P [EC3.1.26.5], putative
Schistosoma mansoni Smp_173000   R-specific enoyl-CoA hydratase
Trypanosoma brucei gambiense Tbg972.8.990   maoC-like dehydratase, putative
Trypanosoma brucei Tb927.8.1440   maoC-like dehydratase, putative
Trypanosoma congolense TcIL3000_8_1180   maoC-like dehydratase, putative
Trypanosoma cruzi TcCLB.508547.50   maoC-like dehydratase, putative

Essentiality

Rv0636 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu648 this record Mycobacterium tuberculosis essential nmpdr
Tb927.8.1440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.1440 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.1440 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.1440 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Rv0636 (Mycobacterium tuberculosis), (3R)-hydroxyacyl-ACP dehydratase subunit HadB
Title for this comment
Comment