Detailed view for EHI_099700

Basic information

TDR Targets ID: 799012
Entamoeba histolytica, NAD(FAD)-dependent dehydrogenase, putative
Source Database / ID:  AmoebaDB  

pI: 6.9317 | Length (AA): 1066 | MW (Da): 116193 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01266   FAD dependent oxidoreductase
PF04324   BFD-like [2Fe-2S] binding domain
PF07892   Protein of unknown function (DUF1667)
PF07992   Pyridine nucleotide-disulphide oxidoreductase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 13 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 495 2rgo (B) 14 606 18.00 0 1 0.582153 0.62
2 44 1rp0 (A) 38 80 35.00 0.68 0.35 0.381038 0.24
3 44 4y4m (A) 36 76 37.00 0.51 0.19 0.3641 0.24
407 462 4e6k (G) 2 54 28.00 0 1 0.514333 -1.67
416 847 1x31 (A) 35 448 17.00 0 1 0.497053 0.92
513 948 1lvl (A) 1 447 18.00 0 1 0.379706 1.29
514 963 5v36 (A) 1 449 15.00 0 1 0.388839 1.23
516 556 3lov (A) 2 42 44.00 0 0.69 0.472262 0.47
516 961 5u8u (A) 3 472 21.00 0 1 0.433086 1.05
519 556 3rha (A) 9 44 64.00 0.1 0.65 0.517347 1.05
672 845 2ywl (A) 1 174 22.00 0.0000018 0.96 0.389927 -0.01
993 1063 2jov (A) 3 75 25.00 0.00037 0.91 0.404304 -0.67
998 1066 3mzf (A) 276 351 29.00 0 0.79 0.262528 0.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127956)

Species Accession Gene Product
Arabidopsis thaliana AT3G56840   FAD-dependent oxidoreductase-like protein
Brugia malayi Bm1_52090   cDNA sequence BC016226
Candida albicans CaO19.13618   similar to several predicted dehydrogenases
Candida albicans CaO19.6238   similar to several predicted dehydrogenases
Caenorhabditis elegans CELE_Y45G12B.3   Protein Y45G12B.3
Dictyostelium discoideum DDB_G0267656   hypothetical protein
Drosophila melanogaster Dmel_CG10639   CG10639 gene product from transcript CG10639-RA
Escherichia coli b2660   L-2-hydroxyglutarate oxidase
Entamoeba histolytica EHI_161010   anaerobic glycerol-3-phosphate dehydrogenase subunit A, putative
Entamoeba histolytica EHI_099700   NAD(FAD)-dependent dehydrogenase, putative
Giardia lamblia GL50803_16125   Glycerol-3-phosphate dehydrogenase
Homo sapiens ENSG00000087299   L-2-hydroxyglutarate dehydrogenase
Leishmania braziliensis LbrM.35.5720   hypothetical protein, conserved
Leishmania donovani LdBPK_365700.1   L-2-hydroxyglutarate dehydrogenase, mitochondrial, putative
Leishmania infantum LinJ.36.5700   hypothetical protein, conserved
Leishmania major LmjF.36.5460   hypothetical protein, conserved
Leishmania mexicana LmxM.36.5460   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_00810   hypothetical protein
Mus musculus ENSMUSG00000020988   L-2-hydroxyglutarate dehydrogenase
Oryza sativa 4327565   Os01g0809900
Schistosoma mansoni Smp_050080   NAD dehydrogenase
Schmidtea mediterranea mk4.004701.07   NAD dehydrogenase, putative
Trypanosoma brucei gambiense Tbg972.10.11450   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.9360   L-2-hydroxyglutarate dehydrogenase, mitochondrial, putative
Trypanosoma congolense TcIL3000_10_8120   L-2-hydroxyglutarate dehydrogenase, mitochondrial, putative
Trypanosoma cruzi TcCLB.509799.140   L-2-hydroxyglutarate dehydrogenase, mitochondrial, putative
Trypanosoma cruzi TcCLB.511037.20   L-2-hydroxyglutarate dehydrogenase, mitochondrial, putative

Essentiality

EHI_099700 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.9360 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.9360 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.9360 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.9360 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2660 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier EHI_099700 (Entamoeba histolytica), NAD(FAD)-dependent dehydrogenase, putative
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