Detailed view for EHI_093770

Basic information

TDR Targets ID: 799057
Entamoeba histolytica, bromodomain-containing protein
Source Database / ID:  AmoebaDB  

pI: 6.5309 | Length (AA): 1163 | MW (Da): 135074 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00439   Bromodomain
PF12157   Protein of unknown function (DUF3591)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

Basal transcription factors (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
368 784 4oy2 (A) 467 902 24.00 0 1 0.383555 1.05
845 1022 4uos (A) 1 178 16.00 0 0.1 0.388052 -1.9
1049 1157 5epb (A) 983 1091 27.00 0 0.98 0.474723 -1.38
1075 1132 5uiy (A) 1454 1511 47.00 0.000031 0.69 0.627771 -0.47

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_129078)

Species Accession Gene Product
Arabidopsis thaliana AT1G32750   histone acetyltransferase of the CBP family 13
Arabidopsis thaliana AT3G19040   transcription initiation factor TFIID subunit 1-B
Brugia malayi Bm1_45470   Bromodomain containing protein
Candida albicans CaO19.13573   TFIID subunit (TBP-associated factor)
Candida albicans CaO19.6193   TFIID subunit (TBP-associated factor)
Candida albicans CaO19.735   TAFII complex component for activated transcription
Candida albicans CaO19.8354   TAFII complex component for activated transcription
Caenorhabditis elegans CELE_W04A8.7   Protein TAF-1
Dictyostelium discoideum DDB_G0292242   HAF group protein
Drosophila melanogaster Dmel_CG17603   TBP-associated factor 1
Echinococcus granulosus EgrG_001020400   transcription initiation factor TFIID subunit 1
Entamoeba histolytica EHI_093770   bromodomain-containing protein
Echinococcus multilocularis EmuJ_001020400  
Homo sapiens ENSG00000122728   TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 210kDa-like
Homo sapiens ENSG00000147133   TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
Loa Loa (eye worm) LOAG_08937   hypothetical protein
Loa Loa (eye worm) LOAG_03359   bromodomain containing protein
Mus musculus ENSMUSG00000031314   TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor
Oryza sativa 4341655   Os06g0645700
Oryza sativa 9266475   Os06g0645800
Saccharomyces cerevisiae YGR274C   Taf1p
Schistosoma japonicum Sjp_0311900   expressed protein
Schistosoma japonicum Sjp_0029640   similar to Transcription initiation factor TFIID 210 kDa subunit, putative
Schistosoma japonicum Sjp_0035050   ko:K03125 transcription initiation factor TFIID subunit D1, putative
Schistosoma mansoni Smp_166840   transcription initiation factor tfiid
Schmidtea mediterranea mk4.023392.01   Transcription initiation factor TFIID subunit 1
Schmidtea mediterranea mk4.023392.00   Transcription initiation factor TFIID subunit 1
Schmidtea mediterranea mk4.010502.01  
Schmidtea mediterranea mk4.019154.00  
Schmidtea mediterranea mk4.017323.00   Transcription initiation factor TFIID subunit 1
Schmidtea mediterranea mk4.017323.01   Transcription initiation factor TFIID subunit 1
Trichomonas vaginalis TVAG_211830   conserved hypothetical protein
Trichomonas vaginalis TVAG_407770   conserved hypothetical protein
Trichomonas vaginalis TVAG_035090   bromodomain-containing protein, putative
Trichomonas vaginalis TVAG_490860   transcription initiation factor tfiid, putative

Essentiality

EHI_093770 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_W04A8.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_W04A8.7 Caenorhabditis elegans larval arrest wormbase
CELE_W04A8.7 Caenorhabditis elegans slow growth wormbase
CELE_W04A8.7 Caenorhabditis elegans sterile wormbase
YGR274C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 210kDa-like Compounds References
Homo sapiens TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0106 0.2744 1
0.0102 0.3196 0.5526

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_093770 (Entamoeba histolytica), bromodomain-containing protein
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