Detailed view for EHI_068560

Basic information

TDR Targets ID: 802039
Entamoeba histolytica, metallo-beta-lactamase family protein
Source Database / ID:  AmoebaDB  

pI: 7.1052 | Length (AA): 217 | MW (Da): 23415 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00753   Metallo-beta-lactamase superfamily

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 215 3tp9 (A) 0 224 21.00 0.00003 1 1.13375 -0.09
5 213 4ysl (A) 5 235 27.00 0.000051 1 1.26953 -0.84
7 215 2xf4 (A) 1 208 27.00 0 1 1.42653 -1.11
7 215 2zwr (A) 1 200 32.00 0 1 1.43653 -1.11
62 186 5ngg (A) 100 238 36.00 0.0000042 0.42 0.609737 0.87

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Rahman HM-1 IMSS Trophozoite. Hon CC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Trophozoite. Hon CC
Show/Hide expression data references
  • Hon CC Transcriptomics of virulent and avirulent strains

Orthologs

Ortholog group members (OG5_127147)

Species Accession Gene Product
Arabidopsis thaliana AT1G53580   persulfide dioxygenase ETHE1-like protein
Brugia malayi Bm1_46955   probable glyoxalase II, 78941-80643
Brugia malayi Bm1_46960   ethe1-prov protein
Caenorhabditis elegans CELE_C33A12.7   Protein ETHE-1
Dictyostelium discoideum DDB_G0291482   beta-lactamase domain-containing protein
Drosophila melanogaster Dmel_CG30022   CG30022 gene product from transcript CG30022-RB
Escherichia coli b0927   putative metal-binding enzyme
Echinococcus granulosus EgrG_001090400   ETHE1 protein
Entamoeba histolytica EHI_068560   metallo-beta-lactamase family protein
Echinococcus multilocularis EmuJ_001090400   ETHE1 protein
Giardia lamblia GL50803_15305   Hydroxyacylglutathione hydrolase
Giardia lamblia GL50803_8913   Hydroxyacylglutathione hydrolase
Homo sapiens ENSG00000105755   ethylmalonic encephalopathy 1
Loa Loa (eye worm) LOAG_00527   hypothetical protein
Mycobacterium leprae ML0493   Possible glyoxalase II (Hydroxyacylglutathione hydrolase) (GLX II)
Mus musculus ENSMUSG00000064254   ethylmalonic encephalopathy 1
Mycobacterium tuberculosis Rv2581c   Possible glyoxalase II (hydroxyacylglutathione hydrolase) (GLX II)
Mycobacterium tuberculosis Rv2260   Conserved hypothetical protein
Mycobacterium ulcerans MUL_1291   hypothetical protein
Mycobacterium ulcerans MUL_1731   Zn-dependent glyoxylase
Oryza sativa 4326319   Os01g0667200
Schistosoma japonicum Sjp_0023140   ko:K01774 ethe1; ethylmalonic encephalopathy 1, putative
Schistosoma mansoni Smp_080670   hypothetical protein
Schistosoma mansoni Smp_165640   beta lactamase domain
Schmidtea mediterranea mk4.012437.00   Persulfide dioxygenase ETHE1, mitochondrial
Schmidtea mediterranea mk4.008670.00   Persulfide dioxygenase ETHE1, mitochondrial
Schmidtea mediterranea mk4.000253.01   Persulfide dioxygenase ETHE1, mitochondrial
Trichomonas vaginalis TVAG_233760   beta lactamase domain, putative
Trichomonas vaginalis TVAG_452590   beta lactamase domain, putative
Trichomonas vaginalis TVAG_019300   beta lactamase domain, putative
Trichomonas vaginalis TVAG_132210   beta lactamase domain, putative
Trichomonas vaginalis TVAG_411410   beta lactamase domain, putative

Essentiality

EHI_068560 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu2295 Mycobacterium tuberculosis essential nmpdr
b0927 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0183 1 1
0.0215 0.5175 0.5
0.0304 0.3722 1
0.0079 0.301 0
0.0208 0.4509 1
0.0101 0.5 0.5
0.0033 0.5 0.5
0.0263 0.5703 1
0.0307 0.4131 1
0.03 0.9089 1
0.0224 1 1
0.0304 0.3722 1
0.0333 0.487 1
0.0161 1 1
0.0151 0.5 0.5
0.0098 0.5 0.5
0.0019 0.5 0.5
0.0173 0.5058 1
0.0284 0.6112 1
0.0051 0.5023 0.5
0.0141 0.5 0.5
0.0139 0.3313 1
0.0068 0.616 0.5
0.0153 1 1
0.032 0.4025 1
0.0134 0.3422 1
0.0282 0.3876 1
0.0259 0.336 1
0.0153 1 1
0.0367 0.531 1
0.03 0.9089 1
0.0115 1 0.5
0.024 0.347 0.5
0.0286 1 0.5
0.0156 1 1
0.0404 0.2792 1
0.0138 0.8072 0
0.035 0.2506 1
0.0088 0.4106 0.5
0.0065 0.5 0.5
0.0031 0.5 0.5
0.0249 0.3365 1
0.0085 0.271 1
0.0101 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier EHI_068560 (Entamoeba histolytica), metallo-beta-lactamase family protein
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