Detailed view for GL50803_9352

Basic information

TDR Targets ID: 814435
Giardia lamblia, U5 small nuclear ribonucleoprotein 200 kDa helicase, putative
Source Database / ID:  GiardiaDB  

pI: 5.3885 | Length (AA): 1594 | MW (Da): 177309 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00270   DEAD/DEAH box helicase
PF00271   Helicase conserved C-terminal domain
PF02889   Sec63 Brl domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 1386 5m52 (A) 150 1664 17.00 0 1 0.933447 0.95
233 431 1wrb (A) 203 393 24.00 0 0.57 0.321543 -0.35
234 427 3ly5 (A) 199 375 23.00 0 0.94 0.398406 -0.88
253 419 1wp9 (A) 25 165 34.00 0.00058 0.61 0.226168 0.4
715 1572 1u6g (C) 27 949 9.00 0 0.75 0.565969 0.72

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126701)

Species Accession Gene Product
Arabidopsis thaliana AT5G61140   U5 small nuclear ribonucleoprotein helicase
Arabidopsis thaliana AT1G20960   putative U5 small nuclear ribonucleoprotein helicase
Arabidopsis thaliana AT2G42270   U5 small nuclear ribonucleoprotein helicase
Babesia bovis BBOV_III000950   sec63 domain containing protein
Babesia bovis BBOV_I001530   helicase, putative
Brugia malayi Bm1_28570   Type III restriction enzyme, res subunit family protein
Brugia malayi Bm1_10275   Type III restriction enzyme, res subunit family protein
Brugia malayi Bm1_43145   U5 small nuclear ribonucleoprotein 200 kDa helicase, putative
Candida albicans CaO19.10610   similar to S. cerevisiae BRR2 (YER172C) spliceosomal U5 snRNP RNA helicase
Candida albicans CaO19.11463   similar to S. cerevisiae SLH1 (YGR271W) putative helicase implicated in translation regulation
Candida albicans CaO19.3098   similar to S. cerevisiae BRR2 (YER172C) spliceosomal U5 snRNP RNA helicase
Candida albicans CaO19.3980   similar to S. cerevisiae SLH1 (YGR271W) putative helicase implicated in translation regulation
Caenorhabditis elegans CELE_Y54E2A.4   Protein Y54E2A.4
Caenorhabditis elegans CELE_Y46G5A.4   Protein SNRP-200
Cryptosporidium hominis Chro.20293   hypothetical protein
Cryptosporidium hominis Chro.50077   hypothetical protein
Cryptosporidium parvum cgd5_2960   U5snrp Brr2 SFII RNA helicase (sec63 and the second part of the RNA
Cryptosporidium parvum cgd2_2770   U5 small nuclear ribonucleoprotein 200kDA helicase, Pre-mRNA splicing helicase BRR2 2 (RNA helicase plus Sec63 domain)
Dictyostelium discoideum DDB_G0270042   DEAD/DEAH box helicase
Dictyostelium discoideum DDB_G0290389   DEAD/DEAH box helicase
Drosophila melanogaster Dmel_CG5205   CG5205 gene product from transcript CG5205-RA
Drosophila melanogaster Dmel_CG5931   lethal (3) 72Ab
Echinococcus granulosus EgrG_001009800   U5 small nuclear ribonucleoprotein 200 kDa
Echinococcus granulosus EgrG_001152700   Activating signal cointegrator 1 complex subunit
Entamoeba histolytica EHI_131080   DEAD/DEAH box helicase, putative
Entamoeba histolytica EHI_045170   U5 snRNP-specific 200kd protein, putative
Echinococcus multilocularis EmuJ_001152700   Activating signal cointegrator 1 complex subunit
Echinococcus multilocularis EmuJ_001009800   U5 small nuclear ribonucleoprotein 200 kDa
Giardia lamblia GL50803_87022   RNA helicase
Giardia lamblia GL50803_9352   U5 small nuclear ribonucleoprotein 200 kDa helicase, putative
Homo sapiens ENSG00000112249   activating signal cointegrator 1 complex subunit 3
Homo sapiens ENSG00000144028   small nuclear ribonucleoprotein 200kDa (U5)
Leishmania braziliensis LbrM.13.0320   RNA helicase, putative
Leishmania braziliensis LbrM.17.0940   ATP-dependent RNA helicase, putative
Leishmania donovani LdBPK_171020.1   ATP-dependent RNA helicase, putative
Leishmania donovani LdBPK_130390.1   ATP-dependent RNA helicase, putative
Leishmania infantum LinJ.17.1030   ATP-dependent RNA helicase, putative
Leishmania infantum LinJ.13.0390   RNA helicase, putative
Leishmania major LmjF.17.0920   ATP-dependent RNA helicase, putative
Leishmania major LmjF.13.0500   RNA helicase, putative
Leishmania mexicana LmxM.17.0920   ATP-dependent RNA helicase, putative
Leishmania mexicana LmxM.13.0500   RNA helicase, putative
Loa Loa (eye worm) LOAG_11428   ASCC3L1 protein
Loa Loa (eye worm) LOAG_05326   U5 small nuclear ribonucleoprotein helicase
Loa Loa (eye worm) LOAG_01392   hypothetical protein
Mus musculus ENSMUSG00000003660   small nuclear ribonucleoprotein 200 (U5)
Mus musculus ENSMUSG00000038774   activating signal cointegrator 1 complex subunit 3
Neospora caninum NCLIV_049050   RNA helicase-related protein required for pre- mRNA splicing (EC 3.6.1.-), related
Neospora caninum NCLIV_065940   hypothetical protein
Oryza sativa 4332045   Os03g0213400
Onchocerca volvulus OVOC11367   Activating signal cointegrator 1 complex subunit 3 homolog
Plasmodium berghei PBANKA_0522900   pre-mRNA-splicing helicase BRR2, putative
Plasmodium berghei PBANKA_1303000   DEAD/DEAH box helicase, putative
Plasmodium falciparum PF3D7_1439100   DEAD/DEAH box helicase, putative
Plasmodium falciparum PF3D7_0422500   pre-mRNA-splicing helicase BRR2, putative
Plasmodium knowlesi PKNH_0514400   pre-mRNA-splicing helicase BRR2, putative
Plasmodium knowlesi PKNH_1243200   DEAD/DEAH box helicase, putative
Plasmodium vivax PVX_090165   pre-mRNA-splicing helicase BRR2, putative
Plasmodium vivax PVX_118535   RNA helicase, putative
Plasmodium yoelii PY05116   Unknown-related
Plasmodium yoelii PY04061   Drosophila melanogaster CG5931 gene product-related
Plasmodium yoelii PY00412   RNA helicase-related
Saccharomyces cerevisiae YGR271W   Slh1p
Saccharomyces cerevisiae YER172C   Brr2p
Schistosoma japonicum Sjp_0081280   ko:K01529 ASCC3, HELIC1; activating signal cointegrator 1 complex subunit 3 [EC:3.6.1.-], putative
Schistosoma japonicum Sjp_0127560   Activating signal cointegrator 1 complex subunit 3, putative
Schistosoma japonicum Sjp_0210030   similar to U5 small nuclear ribonucleoprotein 200 kDa helicase, putative
Schistosoma japonicum Sjp_0090490   Activating signal cointegrator 1 complex subunit 3, putative
Schistosoma japonicum Sjp_0062470   expressed protein
Schistosoma japonicum Sjp_0210000   ko:K01529 ASCC3L1; activating signal cointegrator 1 complex subunit 3-like 1 [EC:3.6.1.-], putative
Schistosoma japonicum Sjp_0124530   Activating signal cointegrator 1 complex subunit 3, putative
Schistosoma japonicum Sjp_0209990   similar to U5 small nuclear ribonucleoprotein 200 kDa helicase, putative
Schistosoma japonicum Sjp_0210010   similar to U5 small nuclear ribonucleoprotein 200 kDa helicase, putative
Schistosoma japonicum Sjp_0210020   similar to U5 small nuclear ribonucleoprotein 200 kDa helicase, putative
Schistosoma mansoni Smp_156920   activating signal cointegrator 1 complex subunit 3 helc1
Schistosoma mansoni Smp_105700   U520
Schmidtea mediterranea mk4.008198.03  
Schmidtea mediterranea mk4.002682.00   Activating signal cointegrator 1 complex subunit 3
Schmidtea mediterranea mk4.002682.01  
Schmidtea mediterranea mk4.018964.01  
Schmidtea mediterranea mk4.043664.00   Activating signal cointegrator 1 complex subunit 3
Schmidtea mediterranea mk4.002244.00   Putative U5 small nuclear ribonucleoprotein 200 kDa helicase
Schmidtea mediterranea mk4.002128.07  
Schmidtea mediterranea mk4.018964.00  
Schmidtea mediterranea mk4.004025.05  
Schmidtea mediterranea mk4.013237.00   Activating signal cointegrator 1 complex subunit 3
Trypanosoma brucei gambiense Tbg972.11.5010   ATP-dependent RNA helicase, putative,DEAD/DEAH box RNA helicase, putative
Trypanosoma brucei gambiense Tbg972.5.3230   ATP-dependent RNA helicase, putative
Trypanosoma brucei Tb927.11.4380   ATP-dependent RNA helicase, putative
Trypanosoma brucei Tb927.5.2290   ATP-dependent RNA helicase, putative
Trypanosoma congolense TcIL3000.11.4440   ATP-dependent RNA helicase, putative
Trypanosoma cruzi TcCLB.507663.50   ATP-dependent RNA helicase, putative
Trypanosoma cruzi TcCLB.511315.9   ATP-dependent RNA helicase, putative
Trypanosoma cruzi TcCLB.511755.60   ATP-dependent RNA helicase, putative
Trypanosoma cruzi TcCLB.511313.40   ATP-dependent RNA helicase, putative
Toxoplasma gondii TGME49_249810   activating signal cointegrator 1 complex subunit 3, putative
Toxoplasma gondii TGME49_223390   activating signal cointegrator 1 complex subunit 3 family 1 ASCC3L1, putative
Theileria parva TP03_0747   ATP-dependent RNA helicase, putative
Theileria parva TP01_0582   RNA helicase, putative
Trichomonas vaginalis TVAG_122950   helicase, putative
Trichomonas vaginalis TVAG_485440   conserved hypothetical protein
Trichomonas vaginalis TVAG_485450   conserved hypothetical protein
Trichomonas vaginalis TVAG_552720   activating signal cointegrator 1 complex subunit 3, helc1, putative
Trichomonas vaginalis TVAG_227750   Antiviral helicase SLH1, putative
Trichomonas vaginalis TVAG_571910   conserved hypothetical protein
Trichomonas vaginalis TVAG_584230   activating signal cointegrator 1 complex subunit 3, helc1, putative
Trichomonas vaginalis TVAG_190620   Antiviral helicase SLH1, putative

Essentiality

GL50803_9352 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.2290 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.5.2290 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.2290 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.2290 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.1930 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.1930 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.1930 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.1930 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y46G5A.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y46G5A.4 Caenorhabditis elegans larval arrest wormbase
CELE_Y46G5A.4 Caenorhabditis elegans larval lethal wormbase
CELE_Y46G5A.4 Caenorhabditis elegans slow growth wormbase
CELE_Y46G5A.4 Caenorhabditis elegans sterile wormbase
YER172C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0522900 Plasmodium berghei Essential plasmo
PBANKA_1303000 Plasmodium berghei Dispensable plasmo
TGME49_223390 Toxoplasma gondii Probably essential sidik
TGME49_249810 Toxoplasma gondii Probably essential sidik
TGME49_223390 Toxoplasma gondii Essentiality uncertain sidik
TGME49_249810 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier GL50803_9352 (Giardia lamblia), U5 small nuclear ribonucleoprotein 200 kDa helicase, putative
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