Detailed view for PBANKA_0931200

Basic information

TDR Targets ID: 828686
Plasmodium berghei, heat shock protein 101
Source Database / ID: 

pI: 9.4551 | Length (AA): 907 | MW (Da): 103201 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)
PF02861   Clp amino terminal domain, pathogenicity island component
PF07724   AAA domain (Cdc48 subfamily)
PF10431   C-terminal, D2-small domain, of ClpB protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0019538   protein metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
35 178 4irf (A) 9 152 65.00 0 1 1.11607 -2.63
36 895 1qvr (A) 4 841 37.00 0 1 1.26448 0.1
39 900 1r6b (X) 2 742 39.00 0 1 1.04799 1.23

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile 4 hs Ring, 16 hs Trophozoite, Erthyrocytic stages. Otto TD Yeoh LM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Ookinete, Female gametocyte, Male gametocyte. Otto TD Yeoh LM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Gametocyte, 22 hs Schizont. Otto TD
Show/Hide expression data references
  • Otto TD A comprehensive evaluation of rodent malaria parasite genomes and gene expression.
  • Yeoh LM Comparative transcriptomics of female and male gametocytes in Plasmodium berghei and the evolution of sex in alveolates.

Orthologs

Ortholog group members (OG5_126636)

Species Accession Gene Product
Arabidopsis thaliana AT2G25140   casein lytic proteinase B4
Arabidopsis thaliana AT5G50920   ATP-dependent Clp protease ATP-binding subunit ClpC
Arabidopsis thaliana AT3G48870   Clp ATPase
Arabidopsis thaliana AT4G14670   hypothetical protein
Arabidopsis thaliana AT1G74310   chaperone protein ClpB1
Arabidopsis thaliana AT5G51070   chaperone protein ClpD
Arabidopsis thaliana AT5G15450   chaperone protein ClpB3
Babesia bovis BBOV_III008980   Clp amino terminal domain containing protein
Babesia bovis BBOV_II004100   ClpB, putative
Candida albicans CaO19.884   similar to S. cerevisiae HSP78 (YDR258C) involved in folding of some mitochondrial proteins
Candida albicans CaO19.13747   heat shock protein 104
Candida albicans CaO19.8503   similar to S. cerevisiae HSP78 (YDR258C) involved in folding of some mitochondrial proteins
Cryptosporidium hominis Chro.70298   clpB protein
Cryptosporidium parvum cgd7_2620   ClpB ATpase (bacterial), signal peptide
Chlamydia trachomatis CT_286   ATP-dependent Clp protease ATP-binding subunit ClpC
Chlamydia trachomatis CT_113   chaperone protein ClpB
Dictyostelium discoideum DDB_G0289047   AAA ATPase domain-containing protein
Dictyostelium discoideum DDB_G0291314   AAA ATPase domain-containing protein
Escherichia coli b2592   protein disaggregation chaperone
Entamoeba histolytica EHI_156560   heat shock protein, putative
Entamoeba histolytica EHI_028920   heat shock protein, putative
Entamoeba histolytica EHI_034710   heat shock protein, putative
Entamoeba histolytica EHI_022620   heat shock protein, putative
Entamoeba histolytica EHI_005657   hypothetical protein
Entamoeba histolytica EHI_072140   heat shock protein, putative
Entamoeba histolytica EHI_017350   heat shock protein, putative
Entamoeba histolytica EHI_183680   heat shock protein 101, putative
Entamoeba histolytica EHI_042860   heat shock protein, putative
Giardia lamblia GL50803_17520   ClpB protein
Homo sapiens ENSG00000162129   ClpB caseinolytic peptidase B homolog (E. coli)
Leishmania braziliensis LbrM.29.1350   ATP-dependent Clp protease subunit, heat shock protein 100 (HSP100), putative,serine peptidase, putative
Leishmania braziliensis LbrM.27.2750   ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78), putative,serine peptidase, putative
Leishmania donovani LdBPK_291360.1   ATP-dependent Clp protease subunit, heat shock protein 100 (HSP100), putative
Leishmania donovani LdBPK_020680.1   ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78), putative
Leishmania infantum LinJ.29.1360   ATP-dependent Clp protease subunit, heat shock protein 100 (HSP100), putative,serine peptidase, putative
Leishmania infantum LinJ.02.0680   ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78), putative,serine peptidase, putative
Leishmania major LmjF.27.2630   ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78), putative,serine peptidase, putative
Leishmania major LmjF.02.0710   ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78), putative,serine peptidase, putative
Leishmania major LmjF.29.1270   ATP-dependent Clp protease subunit, heat shock protein 100 (HSP100), putative,serine peptidase, putative
Leishmania mexicana LmxM.02.0710   ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78), putative,serine peptidase, putative
Leishmania mexicana LmxM.08_29.1270   ATP-dependent Clp protease subunit, heat shock protein 100 (HSP100), putative,serine peptidase, putative
Mycobacterium leprae ML2490c   PROBABLE ENDOPEPTIDASE ATP BINDING PROTEIN (CHAIN B) CLPB (CLPB PROTEIN) (HEAT SHOCK PROTEIN F84.1)
Mycobacterium leprae ML0235   Probable ATP-dependent Clp protease ATP-binding subunit ClpC
Mus musculus ENSMUSG00000001829   ClpB caseinolytic peptidase B
Mycobacterium tuberculosis Rv3596c   Probable ATP-dependent protease ATP-binding subunit ClpC1
Mycobacterium tuberculosis Rv0384c   Probable endopeptidase ATP binding protein (chain B) ClpB (ClpB protein) (heat shock protein F84.1)
Mycobacterium ulcerans MUL_0127   endopeptidase ATP binding protein (chain B) ClpB
Mycobacterium ulcerans MUL_4178   ATP-dependent protease ATP-binding subunit ClpC1
Neospora caninum NCLIV_028370   hypothetical protein
Neospora caninum NCLIV_022360   ATP-dependent Clp protease ATP-binding subunit clpC homolog, chloroplastic (Precursor), related
Neospora caninum NCLIV_037700   hypothetical protein
Neospora caninum NCLIV_007220   hypothetical protein
Neospora caninum NCLIV_023810   ATP-dependent chaperone ClpB, related
Oryza sativa 4335702   Os04g0397100
Oryza sativa 4328515   Os02g0181900
Oryza sativa 4329520   Os02g0526400
Oryza sativa 4339343   Os05g0519700
Oryza sativa 4333161   Os03g0426900
Oryza sativa 4350258   Os11g0267400
Oryza sativa 4351828   Os12g0230100
Plasmodium berghei PBANKA_0931200   heat shock protein 101
Plasmodium berghei PBANKA_0714200   chaperone protein ClpB1, putative
Plasmodium falciparum PF3D7_1116800   heat shock protein 101
Plasmodium falciparum PF3D7_0816600   chaperone protein ClpB1
Plasmodium knowlesi PKNH_0503800   chaperone protein ClpB1, putative
Plasmodium knowlesi PKNH_0914500   heat shock protein 101, putative
Plasmodium vivax PVX_091470   heat shock protein 101, putative
Plasmodium vivax PVX_089580   chaperone protein ClpB1, putative
Plasmodium yoelii PY00565   ClpB protein
Plasmodium yoelii PY05364   clpB protein
Saccharomyces cerevisiae YDR258C   chaperone ATPase HSP78
Saccharomyces cerevisiae YLL026W   chaperone ATPase HSP104
Schistosoma japonicum Sjp_0300960   ko:K03695 ATP-dependent Clp protease ATP-binding subunit ClpB, putative
Schistosoma mansoni Smp_145990   suppressor of potassium transport defect 3 (skd3 protein)
Schmidtea mediterranea mk4.024118.00   Caseinolytic peptidase B protein homolog
Schmidtea mediterranea mk4.002488.00  
Schmidtea mediterranea mk4.028771.00   Caseinolytic peptidase B protein homolog
Trypanosoma brucei gambiense Tbg.972.2.1440   ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78), putative,serine peptidase, putative
Trypanosoma brucei gambiense Tbg.972.2.4200   ATP-dependent Clp protease subunit, heat shock protein 100 (HSP100), putative,serine peptidase, putative
Trypanosoma brucei Tb927.2.3030   ATP-dependent Clp protease subunit, heat shock protein 78 (HSP78), putative
Trypanosoma brucei Tb927.2.5980   chaperone protein ClpB1, putative
Trypanosoma congolense TcIL3000_2_1650   chaperone protein ClpB1, putative
Trypanosoma congolense TcIL3000_2_1630   chaperone protein ClpB1, putative
Trypanosoma congolense TcIL3000_2_370   serine peptidase, putative
Trypanosoma cruzi TcCLB.508665.14   chaperone protein ClpB1, putative
Trypanosoma cruzi TcCLB.509125.20   ATP-dependent Clp protease subunit, heat shock protein 78, putative
Trypanosoma cruzi TcCLB.508737.100   ATP-dependent Clp protease subunit, heat shock protein 78, putative
Toxoplasma gondii TGME49_268650   chaperone clpB protein, putative
Toxoplasma gondii TGME49_282200   ATPase, AAA family protein
Toxoplasma gondii TGME49_202580   ATPase, AAA family protein
Toxoplasma gondii TGME49_275690   ClpB, putative
Treponema pallidum TP0071   ATP-dependent Clp protease subunit B (clpB)
Treponema pallidum TP0549   ATPase (clpC)
Theileria parva TP04_0174   ClpB protein, putative
Theileria parva TP04_0800   ATP-dependent Clp protease ATP-binding subunit, putative
Trichomonas vaginalis TVAG_365820   conserved hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0181   ATP-binding subunit of Clp protease and DnaK/DnaJ chaperones

Essentiality

PBANKA_0931200 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3658 Mycobacterium tuberculosis essential nmpdr
Tb927.2.5980 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.5980 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.2.5980 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.2.5980 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.2.3030 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.3030 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.2.3030 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.2.3030 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2592 Escherichia coli non-essential goodall
PBANKA_0714200 Plasmodium berghei Essential plasmo
PBANKA_0931200 this record Plasmodium berghei Essential plasmo
TGME49_268650 Toxoplasma gondii Essentiality uncertain sidik
TGME49_275690 Toxoplasma gondii Essentiality uncertain sidik
TGME49_202580 Toxoplasma gondii Essentiality uncertain sidik
TGME49_282200 Toxoplasma gondii Essentiality uncertain sidik
TGME49_268650 Toxoplasma gondii Probably non-essential sidik
TGME49_275690 Toxoplasma gondii Probably non-essential sidik
TGME49_202580 Toxoplasma gondii Probably non-essential sidik
TGME49_282200 Toxoplasma gondii Probably non-essential sidik
TGME49_268650 Toxoplasma gondii Probably essential sidik
TGME49_275690 Toxoplasma gondii Probably essential sidik
TGME49_202580 Toxoplasma gondii Probably essential sidik
TGME49_282200 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum cytochrome b-c1 complex subunit 6, putative 88 aa 24.7% 81 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PBANKA_0931200 (Plasmodium berghei), heat shock protein 101
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