Detailed view for TGME49_275970

Basic information

TDR Targets ID: 835855
Toxoplasma gondii, tubulin, putative
Source Database / ID:  ToxoDB 

pI: 8.3647 | Length (AA): 141 | MW (Da): 15680 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0005874   microtubule  
GO:0007017   microtubule-based process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 128 4u3j (B) 297 430 29.00 0 0.93 1.12201 0.83
24 103 2gry (A) 347 431 38.00 0.94 0.04 0.679776 1.38

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_130726)

Species Accession Gene Product
Echinococcus granulosus EgrG_000603100   tubulin epsilon chain
Echinococcus multilocularis EmuJ_000603100   tubulin epsilon chain
Giardia lamblia GL50803_6336   Epsilon tubulin
Homo sapiens ENSG00000074935   tubulin, epsilon 1
Leishmania braziliensis LbrM.21.1240   epsilon tubulin, putative
Leishmania donovani LdBPK_211250.1   epsilon tubulin, putative
Leishmania infantum LinJ.21.1250   epsilon tubulin, putative
Leishmania major LmjF.21.1010   epsilon tubulin, putative
Leishmania mexicana LmxM.21.1010   epsilon tubulin, putative
Mus musculus ENSMUSG00000019845   epsilon-tubulin 1
Neospora caninum NCLIV_007070   tubulin epsilon chain, putative
Plasmodium berghei PBANKA_1300500   tubulin epsilon chain, putative
Plasmodium falciparum PF3D7_1475700   tubulin epsilon chain, putative
Plasmodium knowlesi PKNH_1246000   tubulin epsilon chain, putative
Plasmodium vivax PVX_118660   tubulin epsilon chain, putative
Plasmodium yoelii PY05777   tubulin beta chain
Schistosoma japonicum Sjp_0009290   ko:K10391 tubulin epsilon, putative
Schistosoma mansoni Smp_028360   tubulin subunit epsilon
Schmidtea mediterranea mk4.013167.01   Tubulin epsilon chain
Schmidtea mediterranea mk4.013167.02   Tubulin epsilon chain, putative
Schmidtea mediterranea mk4.016956.01   Tubulin epsilon chain, putative
Schmidtea mediterranea mk4.023212.00   Tubulin epsilon chain, putative
Schmidtea mediterranea mk4.016956.00  
Trypanosoma brucei gambiense Tbg972.10.1240   epsilon tubulin
Trypanosoma brucei Tb927.10.1140   epsilon tubulin
Trypanosoma brucei Tb11.0530   epsilon tubulin
Trypanosoma congolense TcIL3000_10_940   epsilon tubulin
Trypanosoma cruzi TcCLB.509967.160   epsilon tubulin, putative
Trypanosoma cruzi TcCLB.509695.120   epsilon tubulin, putative
Toxoplasma gondii TGME49_275970   tubulin, putative
Toxoplasma gondii TGME49_275870   tubulin/FtsZ family, GTPase domain-containing protein
Trichomonas vaginalis TVAG_065740   tubulin gamma chain, putative

Essentiality

TGME49_275970 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.1140 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.1140 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.1140 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.1140 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1300500 Plasmodium berghei Dispensable plasmo
TGME49_275870 Toxoplasma gondii Probably non-essential sidik
TGME49_275970 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major epsilon tubulin, putative Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_275970 (Toxoplasma gondii), tubulin, putative
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