Detailed view for Sjp_0020330

Basic information

TDR Targets ID: 977386
Schistosoma japonicum, Rho-associated protein kinase 1, putative
Source Database / ID:  Wormbase Parasite  

pI: 5.3877 | Length (AA): 1153 | MW (Da): 131036 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00227   Proteasome subunit
PF10584   Proteasome subunit A N-terminal signature

Gene Ontology

Mouse over links to read term descriptions.
GO:0019773   proteasome core complex, alpha-subunit complex  
GO:0005839   proteasome core complex  
GO:0004298   threonine endopeptidase activity  
GO:0004175   endopeptidase activity  
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0051603   proteolysis involved in cellular protein catabolic process  
GO:0006511   ubiquitin-dependent protein catabolic process  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 17 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 220 1ryp (F) 3 215 26.00 0 1 0.299041 0.63
6 214 1ryp (G) 5 210 36.00 0 1 0.498619 0.61
9 145 5le5 (D) 9 145 66.00 0 1 0.642205 0.67
77 479 4wnk (A) 106 538 22.00 0 1 0.331248 0.75
104 499 5ote (A) 36 417 34.00 0 1 0.439325 0.51
130 744 4kik (B) 1 653 18.00 0.0000000056 1 0.505605 0.7
140 173 3bog (C) 3 69 99.99 0.00000000059 0 1.00156 -0.2
153 390 5ig1 (A) 27 278 26.00 0 1 0.374654 -0.28
214 339 2i6l (A) 102 225 34.00 0.012 0.99 0.384399 0.14
524 738 4tql (A) 23 236 14.00 0 0.52 0.310195 -1.36
687 1086 5h7c (A) 26 372 25.00 0.04 0.99 0.380209 0.3
706 833 4a55 (B) 474 598 28.00 0.57 0.11 0.248091 -0.06
823 980 3l4q (C) 437 590 9.00 0 0.27 0.0989717 -0.81
990 1180 1ryp (M) 0 203 20.00 0.17 0.26 0.0878905 -0.05
992 1174 2z5c (C) 34 219 34.00 0 0.9 0.309122 0.01
996 1180 1ryp (K) 4 188 22.00 0.012 0.46 0.215814 0.12
1064 1175 2z5c (C) 93 220 39.00 0 0.79 0.371055 0.26

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_131020)

Species Accession Gene Product
Brugia malayi Bm1_49370   Protein kinase domain containing protein
Caenorhabditis elegans CELE_C10H11.9   Protein LET-502
Drosophila melanogaster Dmel_CG9774   Rho kinase
Echinococcus granulosus EgrG_001182800   rho-associated protein kinase 1
Echinococcus multilocularis EmuJ_001182800   rho associated protein kinase
Homo sapiens ENSG00000134318   Rho-associated, coiled-coil containing protein kinase 2
Homo sapiens 6093   Rho-associated, coiled-coil containing protein kinase 1
Loa Loa (eye worm) LOAG_00709   AGC/DMPK/ROCK protein kinase
Mus musculus ENSMUSG00000024290   Rho-associated coiled-coil containing protein kinase 1
Mus musculus ENSMUSG00000020580   Rho-associated coiled-coil containing protein kinase 2
Onchocerca volvulus OVOC5274  
Onchocerca volvulus OVOC13427  
Schistosoma japonicum Sjp_0020320   IPR002219,Protein kinase C, phorbol ester/diacylglycerol binding,domain-containing
Schistosoma japonicum Sjp_0020330   Rho-associated protein kinase 1, putative
Schistosoma mansoni Smp_139800   serine/threonine protein kinase
Schmidtea mediterranea mk4.002231.04   Rho-associated kinase
Schmidtea mediterranea mk4.003511.02  

Essentiality

Sjp_0020330 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_C10H11.9 Caenorhabditis elegans embryonic lethal wormbase
CELE_C10H11.9 Caenorhabditis elegans larval arrest wormbase
CELE_C10H11.9 Caenorhabditis elegans larval lethal wormbase
CELE_C10H11.9 Caenorhabditis elegans sterile wormbase
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens Rho-associated, coiled-coil containing protein kinase 1 Compounds References
Homo sapiens Rho-associated, coiled-coil containing protein kinase 2 Compounds References
Rattus norvegicus Rho-associated protein kinase 2 Compounds References
Rattus norvegicus Rho-associated protein kinase 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Sjp_0020330 (Schistosoma japonicum), Rho-associated protein kinase 1, putative
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