Detailed view for ML0395

Basic information

TDR Targets ID: 980026
Mycobacterium leprae, Probable GMP synthase [glutamine-hydrolyzing] GuaA (Glutamine amidotransferase) (GMP synthetase)
Source Database / ID:  Leproma 

pI: 4.803 | Length (AA): 529 | MW (Da): 56691 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Purine metabolism (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 529 1gpm (A) 3 525 47.00 0 1 1.49758 -0.43
15 529 5tw7 (A) 4 521 49.00 0 1 1.53593 -0.69
17 196 1wl8 (A) 3 183 36.00 0 1 0.882365 -1.11
17 529 2ywb (A) 2 503 52.00 0 1 1.53655 -0.46

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127152)

Species Accession Gene Product
Arabidopsis thaliana AT1G63660   putative GMP synthase / glutamine amidotransferase
Babesia bovis BBOV_IV009110   GMP synthase, putative
Brugia malayi Bm1_44235   GMP synthase
Candida albicans CaO19.12276   GMP synthase
Candida albicans CaO19.4813   GMP synthase
Caenorhabditis elegans CELE_M106.4   Protein GMPS-1, isoform C
Cryptosporidium hominis Chro.50499   burgundy CG9242-PA
Cryptosporidium parvum cgd5_4520   GMP synthase
Dictyostelium discoideum DDB_G0281551   GMP synthetase
Drosophila melanogaster Dmel_CG9242   burgundy
Escherichia coli b2507   GMP synthetase (glutamine aminotransferase)
Echinococcus granulosus EgrG_001198000   GMP synthase glutamine hydrolyzing
Echinococcus multilocularis EmuJ_001198000   GMP synthase (glutamine hydrolyzing)
Homo sapiens ENSG00000163655   guanine monphosphate synthase
Leishmania braziliensis LbrM.22.0110   GMP synthase, putative,glutamine amidotransferase, putative
Leishmania donovani LdBPK_220013.1   GMP synthase [glutamine-hydrolysing]
Leishmania infantum LinJ.22.0013   GMP synthase, putative,glutamine amidotransferase, putative
Leishmania major LmjF.22.0110   GMP synthase, putative,glutamine amidotransferase, putative
Leishmania mexicana LmxM.22.0110   GMP synthase, putative,glutamine amidotransferase, putative
Loa Loa (eye worm) LOAG_00204   hypothetical protein
Mycobacterium leprae ML0395   Probable GMP synthase [glutamine-hydrolyzing] GuaA (Glutamine amidotransferase) (GMP synthetase)
Mus musculus 229363   guanine monophosphate synthetase
Mycobacterium tuberculosis Rv3396c   Probable GMP synthase [glutamine-hydrolyzing] GuaA (glutamine amidotransferase) (GMP synthetase)
Mycobacterium ulcerans MUL_0913   GMP synthase
Neospora caninum NCLIV_030970   hypothetical protein
Oryza sativa 4345281   Os08g0326600
Plasmodium berghei PBANKA_1211000   GMP synthase [glutamine-hydrolyzing], putative
Plasmodium falciparum PF3D7_1012600   GMP synthase [glutamine-hydrolyzing]
Plasmodium knowlesi PKNH_0812400   GMP synthase [glutamine-hydrolyzing], putative
Plasmodium vivax PVX_094850   GMP synthetase, putative
Plasmodium yoelii PY03479   GMP synthetase
Saccharomyces cerevisiae YMR217W   GMP synthase (glutamine-hydrolyzing)
Schistosoma japonicum Sjp_0208860   ko:K01951 GMP synthase (glutamine-hydrolysing) [EC6.3.5.2], putative
Schistosoma mansoni Smp_063830   GMP synthetase
Schmidtea mediterranea mk4.038307.00  
Schmidtea mediterranea mk4.002352.00   GMP synthetase
Schmidtea mediterranea mk4.000132.06  
Trypanosoma brucei gambiense Tbg972.7.2290   GMP synthase, putative,glutamine amidotransferase, putative
Trypanosoma brucei Tb927.7.2100   GMP synthase [glutamine-hydrolysing]
Trypanosoma congolense TcIL3000_0_55050   GMP synthase [glutamine-hydrolysing]
Trypanosoma cruzi TcCLB.511807.110   GMP synthase [glutamine-hydrolysing]
Toxoplasma gondii TGME49_230450   bifunctional GMP synthase/glutamine amidotransferase protein
Theileria parva TP01_0862   GMP synthase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0443   GMP synthase

Essentiality

ML0395 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.2100 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.2100 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.2100 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.2100 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2507 Escherichia coli essential goodall
PBANKA_1211000 Plasmodium berghei Slow plasmo
TGME49_230450 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mus musculus guanine monophosphate synthetase Compounds References
Plasmodium falciparum GMP synthase [glutamine-hydrolyzing] Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier ML0395 (Mycobacterium leprae), Probable GMP synthase [glutamine-hydrolyzing] GuaA (Glutamine amidotransferase) (GMP synthetase)
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