Detailed view for Rv2524c

Basic information

TDR Targets ID: 980910
Mycobacterium tuberculosis, Probable fatty acid synthase Fas (fatty acid synthetase)
Source Database / ID:  Tuberculist 

pI: 4.7804 | Length (AA): 3069 | MW (Da): 326222 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00109  
PF00698  
PF01575  
PF02801  
PF08354  

Gene Ontology

Mouse over links to read term descriptions.
GO:0004318   enoyl-[acyl-carrier-protein] reductase (NADH) activity  
GO:0005835   fatty acid synthase complex  
GO:0016740   transferase activity  
GO:0004312   fatty-acid synthase activity  
GO:0003824   catalytic activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  
GO:0006633   fatty acid biosynthetic process  

Metabolic Pathways

Fatty acid biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 1638 2uv8 (G) 130 2050 29.00 0 1 0.644643 0.44
1213 1332 1q6w (A) 30 158 27.00 0 0.96 0.247501 0.24
1219 1315 5cpg (A) 28 136 39.00 0.0022 0.99 0.326106 0.18
1327 1722 3k89 (A) 1 311 25.00 0 1 0.109432 0.48
1328 1716 2c2n (A) 23 339 23.00 0 1 0.159951 0.24
1330 1464 5yda (A) 83 198 32.00 0.13 0.94 0.286488 -0.45
1334 1688 3sbm (A) 4 265 39.00 0.0000000022 1 0.155173 0.64
1639 3061 3hmj (A) 4 1722 34.00 0 1 0.493169 0.94
2111 2146 5ics (A) 4 38 43.00 0.72 1 0.53293 -0.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129123)

Species Accession Gene Product
Candida albicans CaO19.8594   similar to S. cerevisiae FAS1 (YKL182W) fatty acid synthetase beta subunit
Candida albicans CaO19.979   similar to S. cerevisiae FAS1 (YKL182W) fatty acid synthetase beta subunit
Mycobacterium leprae ML1191   PROBABLE FATTY ACID SYNTHASE FAS (FATTY ACID SYNTHETASE)
Mycobacterium tuberculosis Rv2524c   Probable fatty acid synthase Fas (fatty acid synthetase)
Mycobacterium ulcerans MUL_3818   fatty acid synthase Fas
Saccharomyces cerevisiae YKL182W   tetrafunctional fatty acid synthase subunit FAS1
Saccharomyces cerevisiae YPL231W   trifunctional fatty acid synthase subunit FAS2

Essentiality

Rv2524c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
YKL182W Saccharomyces cerevisiae inviable yeastgenome
YPL231W Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0097 0.3189 0.4772
0.0071 0.2948 0
0.0115 0.3243 0.3042
0.0051 0.251 0.1992
0.01 0.3212 0
0.0115 0.3243 0.3042
0.0047 0.2667 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2524c (Mycobacterium tuberculosis), Probable fatty acid synthase Fas (fatty acid synthetase)
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