Detailed view for Rv0715

Basic information

TDR Targets ID: 7054
Mycobacterium tuberculosis, 50S ribosomal protein L24 RplX
Source Database / ID:  Tuberculist 

pI: 11.0737 | Length (AA): 105 | MW (Da): 11475 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00467   KOW motif
PF17136   Ribosomal proteins 50S L24/mitochondrial 39S L24

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 104 5dm6 (R) 14 113 44.00 0 1 1.46528 -0.09
2 36 4zn3 (A) 88 122 34.00 0.049 0.93 0.762133 -0.1
3 105 4wfa (R) 3 101 53.00 0 0.85 1.47105 0.55
4 42 5ohq (A) 1036 1074 28.00 0 0.23 0.708629 -0.53

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_127372)

Species Accession Gene Product
Arabidopsis thaliana AT5G23535   50S ribosomal protein L24-like protein
Arabidopsis thaliana AT5G54600   50S ribosomal protein L24
Babesia bovis BBOV_II007320   50S ribosomal subunit L24, putative
Babesia bovis BBOV_III010490   ribosomal protein L24, putative
Brugia malayi Bm1_11545   KOW motif family protein
Caenorhabditis elegans CELE_F59A3.3   Protein MRPL-24
Chlamydia trachomatis CT_517   50S ribosomal protein L24
Dictyostelium discoideum DDB_G0269640   hypothetical protein
Drosophila melanogaster Dmel_CG8849   mitochondrial ribosomal protein L24
Escherichia coli b3309   50S ribosomal subunit protein L24
Echinococcus granulosus EgrG_000214800   mitochondrial ribosomal protein l24
Echinococcus multilocularis EmuJ_000214800   mitochondrial ribosomal protein l24
Homo sapiens ENSG00000143314   mitochondrial ribosomal protein L24
Leishmania braziliensis LbrM.25.2370   hypothetical protein, conserved
Leishmania donovani LdBPK_251700.1   hypothetical protein, conserved
Leishmania infantum LinJ.25.1700   hypothetical protein, conserved
Leishmania major LmjF.25.1640   hypothetical protein, conserved
Leishmania mexicana LmxM.25.1640   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_06617   KOW domain-containing protein family protein
Mycobacterium leprae ML1848c   PROBABLE 50S RIBOSOMAL PROTEIN L24 RPLX
Mus musculus 67707   mitochondrial ribosomal protein L24
Mycobacterium tuberculosis Rv0715   50S ribosomal protein L24 RplX
Mycobacterium ulcerans MUL_0805   50S ribosomal protein L24
Neospora caninum NCLIV_016340   ribosomal protein L24, putative
Neospora caninum NCLIV_060130   50S ribosomal protein L24, putative
Oryza sativa 4349726   Os11g0139100
Oryza sativa 4341869   Os06g0683200
Oryza sativa 4351443   Os12g0135900
Plasmodium berghei PBANKA_1438800   50S ribosomal protein L24, putative
Plasmodium berghei PBANKA_0103800   50S ribosomal protein L24, putative
Plasmodium falciparum PF3D7_0605000   50S ribosomal protein L24, putative
Plasmodium falciparum PF3D7_1223900   50S ribosomal protein L24, putative
Plasmodium knowlesi PKNH_1145500   50S ribosomal protein L24, putative
Plasmodium knowlesi PKNH_1443100   50S ribosomal protein L24, putative
Plasmodium vivax PVX_123825   50S ribosomal protein L24, putative
Plasmodium vivax PVX_113415   50S ribosomal protein L24, putative
Plasmodium yoelii PY06733   ribosomal protein L24, putative
Schistosoma japonicum Sjp_0041950   Probable 39S ribosomal protein L24, mitochondrial precursor, putative
Schistosoma mansoni Smp_035410   mitochondrial ribosomal protein L24
Schmidtea mediterranea mk4.091311.01  
Schmidtea mediterranea mk4.010078.02   Probable 39S ribosomal protein L24, mitochondrial
Schmidtea mediterranea mk4.010078.01   Probable 39S ribosomal protein L24, mitochondrial
Schmidtea mediterranea mk4.001655.04   Probable 39S ribosomal protein L24, mitochondrial
Trypanosoma brucei gambiense Tbg972.3.1550   hypothetical protein, conserved
Trypanosoma brucei Tb927.3.1710   mitochondrial ribosomal protein l24
Trypanosoma congolense TcIL3000_3_830   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510421.250   hypothetical protein, conserved
Toxoplasma gondii TGME49_216010   ribosomal protein RPL24
Toxoplasma gondii TGME49_239720   50S ribosomal protein l24, putative
Treponema pallidum TP0200   50S ribosomal protein L24
Theileria parva TP02_0094   60S ribosomal protein L24, putative
Theileria parva TP02_0188   60S ribosomal protein L24, putative
Wolbachia endosymbiont of Brugia malayi Wbm0330   50S ribosomal protein L24

Essentiality

Rv0715 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu727 this record Mycobacterium tuberculosis essential nmpdr
Tb927.3.1710 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.1710 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.1710 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.1710 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3309 Escherichia coli essential goodall
CELE_F59A3.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_F59A3.3 Caenorhabditis elegans larval arrest wormbase
PBANKA_1438800 Plasmodium berghei Essential plasmo
TGME49_239720 Toxoplasma gondii Probably essential sidik
TGME49_216010 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0715 (Mycobacterium tuberculosis), 50S ribosomal protein L24 RplX
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