Detailed view for Rv1659

Basic information

TDR Targets ID: 981153
Mycobacterium tuberculosis, Probable argininosuccinate lyase ArgH
Source Database / ID:  Tuberculist 

pI: 4.9271 | Length (AA): 470 | MW (Da): 49742 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00206  
PF14698  

Gene Ontology

Mouse over links to read term descriptions.
GO:0004056   argininosuccinate lyase activity  
GO:0003824   catalytic activity  
GO:0042450   arginine biosynthetic process via ornithine  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 464 1tj7 (A) 3 456 42.00 0 1 1.50774 -0.32
11 467 1i0a (A) 11 462 40.00 0 1 1.48224 -0.41
11 468 6g3e (A) 12 472 32.00 0 1 1.34937 -0.26
15 467 1k62 (A) 15 462 42.00 0 1 1.48773 -0.35
17 464 2e9f (A) 14 459 43.00 0 1 1.47609 -0.27

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127862)

Species Accession Gene Product
Arabidopsis thaliana AT5G10920   putative argininosuccinate lyase
Candida albicans CaO19.6689   arginosuccinate lyase: last step in arginine biosynthesis
Candida albicans CaO19_6689   hypothetical protein
Candida albicans CaO19.13981   arginosuccinate lyase: last step in arginine biosynthesis
Drosophila melanogaster Dmel_CG9510   CG9510 gene product from transcript CG9510-RD
Escherichia coli b3960   argininosuccinate lyase
Homo sapiens ENSG00000126522   argininosuccinate lyase
Mycobacterium leprae ML1413   Probable Argininosuccinate lyase ArgH
Mus musculus ENSMUSG00000025533   argininosuccinate lyase
Mycobacterium tuberculosis Rv1659   Probable argininosuccinate lyase ArgH
Mycobacterium ulcerans MUL_1651   argininosuccinate lyase
Oryza sativa 4332596   Os03g0305500
Saccharomyces cerevisiae YHR018C   argininosuccinate lyase ARG4
Schmidtea mediterranea mk4.058721.00  
Schmidtea mediterranea mk4.000754.09  

Essentiality

Rv1659 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3960 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv1659 (Mycobacterium tuberculosis), Probable argininosuccinate lyase ArgH
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