Detailed view for Rv3566c

Basic information

TDR Targets ID: 6585
Mycobacterium tuberculosis, Arylamine N-acetyltransferase Nat (arylamine acetylase)
Source Database / ID:  Tuberculist 

pI: 5.3596 | Length (AA): 283 | MW (Da): 31029 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00797   N-acetyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016407   acetyltransferase activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 272 4bgf (A) 3 272 99.99 0 1 2.17956 -1.34

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1GX3:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1W5R:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1W6F:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2VFB:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 2VFC:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3LTW:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 4BGF:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase. murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_131280)

Species Accession Gene Product
Escherichia coli b1463   N-hydroxyarylamine O-acetyltransferase
Homo sapiens ENSG00000171428   N-acetyltransferase 1 (arylamine N-acetyltransferase)
Homo sapiens ENSG00000156006   N-acetyltransferase 2 (arylamine N-acetyltransferase)
Mus musculus ENSMUSG00000051147   N-acetyltransferase 2 (arylamine N-acetyltransferase)
Mus musculus ENSMUSG00000025588   N-acetyl transferase 1
Mycobacterium tuberculosis Rv3566c   Arylamine N-acetyltransferase Nat (arylamine acetylase)
Mycobacterium ulcerans MUL_4131   arylamine N-acetyltransferase Nat

Essentiality

Rv3566c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b1463 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
response to drug (GO:0042493) decreased sensitivity toward (PATO:0001550) inferred from over expression (ECO:0000120) Mycobacterium tuberculosis 5089   216208  
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 9973365
response to drug (GO:0042493) decreased sensitivity toward (PATO:0001550) inferred from over expression (ECO:0000120) Mycobacterium smegmatis 5089   216208  
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 9973365
growth (GO:0040007) decreased (PATO:0000468) inferred from loss-of-function mutant phenotype (ECO:0000016) Mycobacterium smegmatis No drug identifiers listed for this gene.
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 11739761
response to drug (GO:0042493) increased sensitivity toward (PATO:0001549) inferred from loss-of-function mutant phenotype (ECO:0000016) Mycobacterium smegmatis 5089   216208  
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 11739761
growth (GO:0040007) decreased (PATO:0000468) inferred from loss-of-function mutant phenotype (ECO:0000016) Mycobacterium bovis No drug identifiers listed for this gene.
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 15117974
morphology (PATO:0000051) abnormal (PATO:0000460) inferred from loss-of-function mutant phenotype (ECO:0000016) Mycobacterium bovis No drug identifiers listed for this gene.
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 15117974
lipid biosynthetic process (GO:0008610) abnormal (PATO:0000460) inferred from loss-of-function mutant phenotype (ECO:0000016) Mycobacterium bovis No drug identifiers listed for this gene.
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 15117974
response to drug (GO:0042493) increased sensitivity toward (PATO:0001549) inferred from loss-of-function mutant phenotype (ECO:0000016) Mycobacterium bovis 5089   152492   216208   306201   338772   339299   543699   546666  
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 15117974
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Mycobacterium smegmatis 0  
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 17368035
growth (GO:0040007) decreased (PATO:0000468) inferred from in vitro culture assay (ECO:0000182) Mycobacterium bovis 0  
Annotator: crowther@u.washington.edu. Comment: 2007-12-21. References: 17368035

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG12228) Arylamine N-acetyltransferase Compounds References
Mycobacterium smegmatis Arylamine N-acetyltransferase Compounds References
Mus musculus N-acetyltransferase 2 (arylamine N-acetyltransferase) Compounds References
Homo sapiens N-acetyltransferase 1 (arylamine N-acetyltransferase) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.1241 0.5563 1
0.4493 0.899 1
0.0649 0.893 1
0.0583 0.8359 1
0.458 0.5265 1
0.0613 0.6926 1
0.0611 0.4886 0.9319
0.7822 0.728 1
0.1274 0.4902 1
0.0641 0.2679 1
0.0642 0.785 1
0.0619 0.3538 0.8704
0.0623 0.7531 1
0.0629 0.2627 1
0.0613 0.2735 1
0.458 0.5265 1
0.1239 0.8304 1
0.214 0.6531 1
0.1727 0.5949 1
0.0623 0.3072 1
1.216 0.3586 1
0.0629 0.2627 1
0.1276 0.8288 1
0.458 0.5265 1
0.0623 0.7358 1
0.1262 0.4447 1
0.458 0.5265 1
0.0621 0.7817 1
0.0644 0.7046 0.9249
0.4712 0.3915 0.3915
0.0636 0.4522 1
0.7822 0.728 1
0.0626 0.3591 0.9258
0.4493 0.899 1
0.214 0.6531 1
0.0763 0.8154 1
0.3822 0.7579 1
0.0623 0.7531 1
0.1922 0.5252 0.9663
0.0617 0.3356 0.5515
0.0629 0.3047 1
0.0617 0.4891 1
0.1103 0.5201 1
0.0629 0.2627 1
0.2612 0.9188 1
0.1261 0.7907 1
0.7822 0.728 1
0.0633 0.3069 0.6674
0.0614 0.7341 1
0.0615 0.678 1
0.0618 0.699 0.9308
0.3938 0.2823 1
0.1296 0.3828 0.9328
0.1273 0.5849 1
0.0618 0.3718 1
0.0614 0.6935 0.9111
0.1271 0.9049 1
0.0624 0.2727 1
0.5011 0.3126 0.3126
0.1261 0.2835 1
0.1296 0.3773 1
0.452469 1 1
0.0625 0.7829 1
0.191974 1 1
0.0625 0.8168 0.5
0.1943 0.5615 1
0.7036 0.4965 1
0.0628 0.3837 1
0.1297 0.4145 1
0.0652 0.7921 1
0.0632 0.5063 1
0.0644 0.7046 0.9249
0.0641 0.3016 1
0.458 0.5265 1
0.0617 0.31 0.6827
0.0626 0.3591 0.9258
0.1943 0.5615 1
0.1297 0.4145 1
0.0619 0.4749 1
0.458 0.5265 1
0.325907 1 1
0.7822 0.728 1
0.0628 0.3837 1
0.1103 0.5201 1
0.7822 0.728 1
0.0614 0.5987 1
0.7822 0.728 1
0.1241 0.4548 1
0.7822 0.728 1
0.1285 0.411 0.411
0.0631 0.3116 0.9277
0.0679128 1 1
0.0616 0.7051 0.9261
0.0627 0.5485 1
0.191 0.5438 0.5435
0.0614 0.7586 1
0.0639 0.7336 0.9217
0.0626 0.705 0.926
0.0644 0.7046 0.9249
0.1261 0.2835 1
0.7822 0.728 1
0.1253 0.3749 0.3748
0.1262 0.9338 1
0.563 0.6763 1
0.0707 0.2788 1
0.1365 0.3353 0.8364
0.0611 0.7372 0.9278
0.0614 0.428 1
0.3938 0.2823 1
0.0622 0.5765 0.893
0.1431 0.8025 1
0.7822 0.728 1
0.1247 0.5775 1
0.0763 0.7508 0.5
0.0644 0.7046 0.9249
0.0576 0.3265 0.3265
0.2117 0.8935 1
0.1266 0.3522 1
0.0624 0.3967 1
0.458 0.5265 1
0.0583 0.8359 1
0.458 0.5265 1
0.1273 0.5849 1
0.0258 0.3596 1
0.1263 0.9302 1
0.0612 0.7275 0.9288
0.0613 0.2735 1
0.1431 0.8025 1
0.0611 0.7226 0.9086
0.1284 0.346 1
0.0622 0.5765 0.893
0.7036 0.4965 1
0.1273 0.5849 1
1.216 0.3586 1
0.3247 0.984 1
0.1241 0.5563 1
0.0622 0.5765 0.893
0.0611 0.2781 1
0.1979 0.6 1
0.0632 0.778 1
0.062 0.3968 1
0.0622 0.5765 0.893
0.1365 0.3353 0.8364
0.1252 0.2826 1
0.063 0.559 1
0.1464 0.9667 1
0.7822 0.728 1
0.0642 0.945 1
0.458 0.5265 1
0.0618 0.7773 1
0.192226 0.516987 0.964159
0.325907 1 1
0.0611 0.9601 1
0.0612 0.7275 0.9288
0.129 0.7246 1
0.0763 0.3331 1
0.1969 0.5476 0.5913
0.4472 0.3913 0.3912
0.458 0.5265 1
0.1276 0.8289 1
1.216 0.3586 1
0.4493 0.899 1
0.1241 0.5563 1
0.0874 0.4248 1
0.0631 0.3116 0.9277
0.7036 0.4965 1
0.0763 0.6069 0.5
0.191 0.5438 0.5435
0.7822 0.728 1
0.1263 0.7723 1
0.458 0.5265 1
0.7822 0.728 1
0.1238 0.8288 1
0.2119 0.8071 1
0.0629 0.4229 0.4541
0.7822 0.728 1
0.0612 0.2908 1
0.1302 0.2829 1
0.0864 0.2633 1
0.2119 0.8071 1
0.458 0.5265 1
0.214 0.6531 1
0.1256 0.7434 1
0.7822 0.728 1
0.1293 0.4216 1
0.0612 0.4056 1
0.0629 0.2627 1
0.0633 0.3069 0.6674
0.0644 0.7046 0.9249
0.1969 0.5476 0.5913
0.0657 0.4681 1
0.0641 0.4725 1
0.0629 0.7825 1
0.1887 0.7792 1
0.1293 0.4216 1
0.7822 0.728 1
0.1969 0.5476 0.5913
0.0624 0.2727 1
0.0278068 0.812322 1
0.067 0.9064 1
0.2597 0.2562 1
0.0642 0.785 1
0.0652 0.4595 1
0.1281 0.915 1
0.0874 0.9168 0.9166
0.062 0.773 1
0.0618 0.7727 1
0.0622 0.5765 0.893
0.0644 0.3003 1
0.563 0.6763 1
0.7822 0.728 1
0.458 0.5265 1
0.4493 0.899 1
0.0629 0.2827 1
0.214 0.6531 1
0.0619 0.3538 0.8704
0.458 0.5265 1
0.0614 0.8842 1
0.0617 0.285 1
0.458 0.5265 1
0.0763 0.3014 1
0.0637 0.8806 1
0.0622 0.5765 0.893
0.0629 0.2627 1
0.0652 0.2625 1
0.0625 0.8168 0.5
0.1285 0.411 0.411
0.7822 0.728 1
0.7822 0.728 1
0.7822 0.728 1
0.0623 0.3084 1
0.1253 0.3749 0.3748
0.1303 0.6356 0.7352
0.1241 0.8055 1
0.0611 0.7224 0.9082
0.1294 0.9148 1
0.191 0.5438 0.5435
0.3845 0.8431 1
0.1257 0.8288 1
0.0954 0.4506 1
0.0669 0.3052 1
0.1277 0.2788 1
0.062 0.5735 1
0.1273 0.5849 1
0.0613 0.2735 1
0.0639 0.7339 0.9219
0.0635 0.2696 1
0.0916 0.5755 1
0.0657 0.8301 1
0.0652 0.2625 1
0.7822 0.728 1
0.1241 0.5563 1
0.0615 0.4974 1
0.7036 0.4965 1
0.1262 0.7771 1
0.1431 0.8025 1
0.1355 0.6329 1
0.3845 0.8431 1
0.1251 0.4258 1
0.1455 0.9721 1
0.458 0.5265 1
0.2897 0.9611 1
0.1979 0.6 1
0.0626 0.4209 1
0.191 0.5438 0.5435
0.1293 0.4216 1
0.0622 0.5765 0.893
0.1285 0.411 0.411
0.1293 0.4216 1
0.0802 0.7217 1
0.0639 0.7336 0.9217
0.0652 0.3122 0.9231
0.0635 0.2902 1
0.4493 0.899 1
0.0644 0.7046 0.9249
0.191 0.5438 0.5435
0.1526 0.4748 0.5
0.0631 0.9455 1
0.7822 0.728 1
0.1887 0.7792 1
0.0649 0.408 1
0.1262 0.3711 1
0.7036 0.4965 1
0.124086 0.834699 1
0.0611 0.6951 1
0.0622 0.5765 0.893
0.1365 0.3353 0.8364
0.0616 0.7814 1
0.7822 0.728 1
0.7036 0.4965 1
0.012 0.6661 0.6661
0.1922 0.5252 0.9663
0.4915 0.9027 0.9027
0.0611 0.9108 0.9105
0.0652 0.3122 0.9231
0.0625 0.5596 1
0.2011 0.686 1
0.0617 0.332 1
0.1943 0.5615 1
0.129 0.7246 1
0.1365 0.3353 0.8364
0.458 0.5265 1
0.1251 0.4526 1
0.062 0.439 1
0.1969 0.5476 0.5913
0.0624 0.4452 1
0.0642 0.945 1
0.1251 0.4258 1
0.0619 0.7398 1
0.7822 0.728 1
0.0763 0.7508 0.5
0.1261 0.7907 1
0.0763 0.4005 0.5
0.125 0.8053 1
0.7822 0.728 1
0.1257 0.8286 1
0.0631 0.5129 1
0.0763 0.4005 0.5
0.0642 0.2709 1
0.0631 0.3608 1
0.191 0.5438 0.5435
0.0626 0.3591 0.9258
0.0619 0.738 1
0.1277 0.2788 1
0.1273 0.5849 1

Assayability

Assay information

  • Assay for Arylamine Acetyltransferase (2.3.1.5 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Mycobacterium smegmatis ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Rv3566c cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Mycobacterium tuberculosis ( 1 )

Bibliographic References

53 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Rv3566c (Mycobacterium tuberculosis), Arylamine N-acetyltransferase Nat (arylamine acetylase)
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