Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Plasmodium falciparum | dihydroorotate dehydrogenase | Starlite/ChEMBL | References |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
Activity (functional) | = 56 % | Antimalarial activity against erythrocytic stage of Plasmodium berghei NK65 infected in BALB/c mouse assessed as reduction in parasitemia at 50 mg/kg, po administered QD for 4 days starting 1 day post-infection measured 24 hrs post-last dose relative to vehicle-treated control | ChEMBL. | 22877245 |
Cp (ADMET) | < 0.003 uM | Plasma concentration in Swiss mouse at 10 mg/kg, po at 16 hrs | ChEMBL. | 22877245 |
EC50 (functional) | = 0.32 uM | Antiplasmodial activity against Plasmodium falciparum 3D7 infected in human erythrocytes assessed as inhibition of [3H]hypoxanthine incorporation after 48 hrs | ChEMBL. | 22877245 |
IC50 (binding) | = 77 nM | BindingDB_Patents: Inhibiton Assay. For studying inhibition of Plasmodium or human DHODH enzyme, two assays that are in routine use are described, for example, in Baldwin, et al. (2002) JBiol Chem., 277, 41827-41834, and Baldwin, et al. (2005) J. Biol. Chem., 280. 21847-21853.Briefly, this colorimetric assay monitors the reduction of 2,6-dichloroindophenol (DCIP) at 600 nm (e = 18.8 mM-1cm-1) for measuring DHOD inhibition. The assay was carried out using a solution containing 100 mM HEPES, pH 8.0, 150 mM NaCl, 10% glycerol, 0.1% Triton X-100, 20 micro molar CoQD (coenzyme QD), 200 micro molar L-dihydroorotate, and 120 micro molar DCIP. Reactions are initiated by addition of enzyme to a final concentration in the range of about 5 nM to about 50 nM while maintaining the temperature of a circulating water bath at 25° C. Alternatively, for potent compounds, activity was determined by directly measuring the production of orotic acid at 296 nm (ε = 4.3 mM-1 cm-1). | ChEMBL. | No reference |
IC50 (binding) | = 0.077 uM | Inhibition of Plasmodium falciparum DHODH expressed in Escherichia coli DH5alpha using 2,6-dichoroindophenol substrate by spectrophotometric analysis | ChEMBL. | 22877245 |
IC50 (functional) | = 0.36 uM | Antimalarial activity: Asexual Blood Stage Plasmodium falciparum W2 IC50 (uM) | ChEMBL. | No reference |
IC50 (functional) | = 0.38 uM | Antimalarial activity: Asexual Blood Stage Plasmodium falciparum 3D7 IC50 (uM) | ChEMBL. | No reference |
IC50 (binding) | > 100 uM | Inhibition of human DHODH expressed in Escherichia coli BL21(DE3) using 2,6-dichoroindophenol substrate by spectrophotometric analysis | ChEMBL. | 22877245 |
Inhibition (binding) | Compound was evaluated for the inhibition of human FECH at 10uM | MMV_PBOX. | No reference | |
Inhibition (functional) | = | Antimalarial activity: Gametocytes Plasmodium falciparum NF54 Gametocytes Stage V: inhibition at 10 uM (%) | ChEMBL. | No reference |
Inhibition (functional) | = | Antimalarial activity: Liver Stage Plasmodium berghei sporozoite Luciferase assay: inhibition at 10 uM (%) | ChEMBL. | No reference |
Species name | Source | Reference | Is orphan |
---|---|---|---|
Plasmodium falciparum | ChEMBL23 | 22877245 |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.
1 literature reference was collected for this gene.