Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | acetyl-CoA carboxylase beta | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Trypanosoma brucei | NADPH-dependent diflavin oxidoreductase 1 | 0.0055 | 0.3904 | 0.3904 |
Toxoplasma gondii | acetyl-coA carboxylase ACC2 | 0.0101 | 1 | 1 |
Leishmania major | NADPH-cytochrome p450 reductase-like protein | 0.0055 | 0.3904 | 0.2619 |
Echinococcus multilocularis | propionyl coenzyme A carboxylase alpha chain | 0.0039 | 0.1741 | 0.0686 |
Loa Loa (eye worm) | carboxyl transferase domain-containing protein | 0.0098 | 0.9533 | 1 |
Toxoplasma gondii | acetyl-CoA carboxylase ACC1 | 0.0101 | 1 | 1 |
Trypanosoma brucei | acetyl-CoA carboxylase | 0.0101 | 1 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0055 | 0.3904 | 0.3299 |
Schistosoma mansoni | acetyl-CoA carboxylase | 0.0101 | 1 | 1 |
Echinococcus granulosus | NADPH cytochrome P450 reductase | 0.0055 | 0.3904 | 0.3125 |
Plasmodium vivax | NADPH-cytochrome p450 reductase, putative | 0.0055 | 0.3904 | 0.2548 |
Schistosoma mansoni | pyruvate carboxylase | 0.0039 | 0.1741 | 0.1525 |
Plasmodium vivax | biotin carboxylase subunit of acetyl CoA carboxylase, putative | 0.0073 | 0.6314 | 1 |
Chlamydia trachomatis | biotin carboxylase | 0.0035 | 0.1274 | 1 |
Schistosoma mansoni | methylcrotonyl-CoA carboxylase | 0.0039 | 0.1741 | 0.1525 |
Trypanosoma brucei | 3-methylcrotonyl-CoA carboxylase alpha subunit, putative | 0.0039 | 0.1741 | 0.1741 |
Toxoplasma gondii | pyruvate carboxylase | 0.0039 | 0.1741 | 0.1525 |
Plasmodium falciparum | biotin carboxylase subunit of acetyl CoA carboxylase, putative | 0.0073 | 0.6314 | 1 |
Mycobacterium ulcerans | formate dehydrogenase H FdhF | 0.0055 | 0.3904 | 1 |
Trypanosoma cruzi | p450 reductase, putative | 0.0055 | 0.3904 | 0.6808 |
Echinococcus granulosus | propionyl coenzyme A carboxylase alpha chain | 0.0039 | 0.1741 | 0.0686 |
Trypanosoma cruzi | acetyl-CoA carboxylase | 0.0063 | 0.4918 | 1 |
Mycobacterium tuberculosis | Probable acetyl-/propionyl-coenzyme A carboxylase alpha chain (alpha subunit) AccA2: biotin carboxylase + biotin carboxyl carrie | 0.0039 | 0.1741 | 0.5 |
Leishmania major | cytochrome P450 reductase, putative | 0.0049 | 0.3081 | 0.1622 |
Brugia malayi | flavodoxin family protein | 0.0055 | 0.3904 | 0.3299 |
Schistosoma mansoni | methylcrotonyl-CoA carboxylase | 0.0039 | 0.1741 | 0.1525 |
Trypanosoma brucei | NADPH--cytochrome P450 reductase, putative | 0.0055 | 0.3904 | 0.3904 |
Trypanosoma cruzi | cytochrome P450 reductase, putative | 0.0055 | 0.3904 | 0.6808 |
Giardia lamblia | Nitric oxide synthase, inducible | 0.0049 | 0.3081 | 0.5 |
Trichomonas vaginalis | sulfite reductase, putative | 0.0055 | 0.3904 | 1 |
Leishmania major | p450 reductase, putative | 0.0055 | 0.3904 | 0.2619 |
Echinococcus multilocularis | NADPH cytochrome P450 reductase | 0.0055 | 0.3904 | 0.3125 |
Mycobacterium tuberculosis | Probable pyruvate carboxylase Pca (pyruvic carboxylase) | 0.0039 | 0.1741 | 0.5 |
Trypanosoma cruzi | cytochrome P450 reductase, putative | 0.0055 | 0.3904 | 0.6808 |
Schistosoma mansoni | cytochrome P450 reductase | 0.0055 | 0.3904 | 0.3745 |
Wolbachia endosymbiont of Brugia malayi | Acetyl/propionyl-CoA carboxylase, alpha subunit | 0.0039 | 0.1741 | 0.5 |
Trypanosoma brucei | 3-methylcrotonyl-CoA carboxylase alpha subunit, putative | 0.0039 | 0.1741 | 0.1741 |
Leishmania major | acetyl-CoA carboxylase, putative | 0.0101 | 1 | 1 |
Trypanosoma brucei | NADPH-cytochrome p450 reductase, putative | 0.0055 | 0.3904 | 0.3904 |
Echinococcus multilocularis | NADPH dependent diflavin oxidoreductase 1 | 0.0055 | 0.3904 | 0.3125 |
Brugia malayi | FAD binding domain containing protein | 0.0055 | 0.3904 | 0.3299 |
Loa Loa (eye worm) | FAD binding domain-containing protein | 0.0055 | 0.3904 | 0.3299 |
Mycobacterium leprae | Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase, alpha chain AccA3 (BccP) | 0.0039 | 0.1741 | 0.5 |
Brugia malayi | Carboxyl transferase domain containing protein | 0.0098 | 0.9533 | 1 |
Schistosoma mansoni | NADPH flavin oxidoreductase | 0.0028 | 0.0309 | 0.0057 |
Echinococcus granulosus | NADPH dependent diflavin oxidoreductase 1 | 0.0055 | 0.3904 | 0.3125 |
Giardia lamblia | Hypothetical protein | 0.0049 | 0.3081 | 0.5 |
Schistosoma mansoni | 5-methyl tetrahydrofolate-homocysteine methyltransferase reductase | 0.0034 | 0.1133 | 0.0902 |
Trypanosoma cruzi | NADPH-dependent FMN/FAD containing oxidoreductase, putative | 0.0055 | 0.3904 | 0.6808 |
Trypanosoma brucei | NADPH--cytochrome P450 reductase, putative | 0.0055 | 0.3904 | 0.3904 |
Echinococcus multilocularis | acetyl coenzyme A carboxylase 1 | 0.0101 | 1 | 1 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 198 nM | BindingDB_Patents: Spectrophotometric 384 Well Assay. Malonyl CoA formation by acetyl CoA carboxylases is stoichometrically linked to the consumption of ATP. ACC2 activity is measured in a NADH-linked kinetic method measuring ADP generated during the ACC reaction using a coupled lactate dehydrogenase/pyruvate kinase reaction.For biological testing, a human ACC2 construct which lacks the 128 amino acids at the N-terminus for increased solubility (nt 385-6966 in Genbank entry AJ575592) is cloned. The protein is then expressed in insect cells using a baculoviral expression system. Protein purification is performed by anion exchange.All compounds are dissolved in dimethyl sulfoxide (DMSO) to a concentration of 10 mM.Assay reactions are then carried out in 384-well plates, with hACC2 in an appropriate dilution and at final assay concentrations (f.c.) of 100 mM Tris (pH 7.5), 10 mM trisodium citrate, 25 mM KHCO3, 10 mM MgCl2, 0.5 mg/mL BSA, 3.75 mM reduced L-glutathione, 15 U/mL lactate dehydrogenase, 0.5 mM phosphoenolpyruvate. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.