Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus multilocularis | protein kinase c epsilon type | 0.0865 | 0.9707 | 0.9825 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0877 | 0.988 | 1 |
Loa Loa (eye worm) | AGC/PKC/ETA protein kinase | 0.0865 | 0.9707 | 0.9701 |
Brugia malayi | protein kinase C II. | 0.0865 | 0.9707 | 1 |
Trichomonas vaginalis | AGC family protein kinase | 0.0246 | 0.0542 | 0.3112 |
Loa Loa (eye worm) | AGC/PKA protein kinase | 0.0291 | 0.1219 | 0.1028 |
Echinococcus granulosus | cAMP dependent protein kinase catalytic subunit | 0.0291 | 0.1219 | 0.1234 |
Echinococcus granulosus | serine:threonine protein kinase N2 | 0.0236 | 0.0399 | 0.0404 |
Echinococcus granulosus | ribosomal protein S6 kinase alpha 3 | 0.0236 | 0.0399 | 0.0404 |
Loa Loa (eye worm) | hypothetical protein | 0.0649 | 0.6508 | 0.6433 |
Trichomonas vaginalis | AGC family protein kinase | 0.0246 | 0.0542 | 0.3112 |
Plasmodium falciparum | cAMP-dependent protein kinase catalytic subunit | 0.0291 | 0.1219 | 0.5 |
Trypanosoma cruzi | cAMP-dependent protein kinase catalytic subunit 1 | 0.0291 | 0.1219 | 0.5 |
Brugia malayi | Protein kinase domain containing protein | 0.0246 | 0.0542 | 0.0558 |
Entamoeba histolytica | PH domain containing protein kinase, putative | 0.0301 | 0.1362 | 0.3423 |
Mycobacterium ulcerans | glycosyl hydrolase | 0.0225 | 0.0236 | 0.5 |
Schistosoma mansoni | atypical protein kinase C | 0.0712 | 0.7442 | 0.7532 |
Trypanosoma brucei | cAMP-dependent protein kinase catalytic subunit 1 | 0.0291 | 0.1219 | 1 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0291 | 0.1219 | 0.1234 |
Brugia malayi | Hr1 repeat family protein | 0.0236 | 0.0399 | 0.0411 |
Loa Loa (eye worm) | hypothetical protein | 0.0485 | 0.4078 | 0.3949 |
Echinococcus multilocularis | serine:threonine protein kinase N2 | 0.0642 | 0.6402 | 0.648 |
Brugia malayi | Protein kinase domain containing protein | 0.0236 | 0.0399 | 0.0411 |
Loa Loa (eye worm) | hypothetical protein | 0.0616 | 0.6019 | 0.5933 |
Loa Loa (eye worm) | CAMK/PKD protein kinase | 0.0297 | 0.1299 | 0.111 |
Echinococcus granulosus | serine:threonine protein kinase | 0.0239 | 0.0441 | 0.0447 |
Schistosoma mansoni | serine/threonine kinase | 0.0236 | 0.0399 | 0.0404 |
Echinococcus multilocularis | unc 13 (munc13) | 0.0223 | 0.0213 | 0.0215 |
Trypanosoma cruzi | cAMP-dependent protein kinase catalytic subunit 2 | 0.0291 | 0.1219 | 0.5 |
Schistosoma mansoni | unc-13 (munc13) | 0.0223 | 0.0213 | 0.0215 |
Trichomonas vaginalis | AGC family protein kinase | 0.0246 | 0.0542 | 0.3112 |
Loa Loa (eye worm) | hypothetical protein | 0.0291 | 0.1219 | 0.1028 |
Echinococcus multilocularis | phosphorylase b kinase regulatory | 0.0532 | 0.4781 | 0.4839 |
Loa Loa (eye worm) | AGC/RSK/RSK protein kinase | 0.0236 | 0.0399 | 0.0191 |
Schistosoma mansoni | phosphorylase B kinase alpha kpb1 | 0.0532 | 0.4781 | 0.4839 |
Onchocerca volvulus | Serine\/threonine kinase homolog | 0.0318 | 0.161 | 0.2219 |
Onchocerca volvulus | 0.0485 | 0.4078 | 0.6139 | |
Onchocerca volvulus | Phosphorylase b kinase regulatory subunit beta homolog | 0.0357 | 0.2188 | 0.3138 |
Echinococcus granulosus | protein kinase c epsilon type | 0.0865 | 0.9707 | 0.9825 |
Loa Loa (eye worm) | AGC/PKC/ALPHA protein kinase | 0.0453 | 0.361 | 0.3471 |
Echinococcus multilocularis | serine:threonine protein kinase 38 | 0.0236 | 0.0399 | 0.0404 |
Echinococcus multilocularis | phosphorylase b kinase regulatory | 0.0357 | 0.2188 | 0.2215 |
Schistosoma mansoni | protein kinase C | 0.0223 | 0.0213 | 0.0215 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0877 | 0.988 | 1 |
Toxoplasma gondii | AGC kinase | 0.0291 | 0.1219 | 1 |
Brugia malayi | hypothetical protein | 0.0532 | 0.4781 | 0.4926 |
Loa Loa (eye worm) | hypothetical protein | 0.0847 | 0.9444 | 0.9432 |
Loa Loa (eye worm) | CAMK/PKD protein kinase | 0.0495 | 0.4235 | 0.4109 |
Loa Loa (eye worm) | hypothetical protein | 0.0307 | 0.1453 | 0.1267 |
Toxoplasma gondii | protein kinase, cAMP-dependent, catalytic chain | 0.0291 | 0.1219 | 1 |
Brugia malayi | Protein kinase c protein 2 | 0.0651 | 0.6546 | 0.6743 |
Brugia malayi | hypothetical protein | 0.0225 | 0.0236 | 0.0243 |
Echinococcus multilocularis | ribosomal protein s6 kinase beta 1 | 0.0236 | 0.0399 | 0.0404 |
Echinococcus multilocularis | cAMP dependent protein kinase catalytic subunit | 0.0291 | 0.1219 | 0.1234 |
Echinococcus multilocularis | serine threonine protein kinase | 0.0678 | 0.6945 | 0.7029 |
Echinococcus multilocularis | Ribosomal protein S6 kinase beta 2 | 0.0236 | 0.0399 | 0.0404 |
Brugia malayi | p70 ribosomal S6 kinase beta | 0.0236 | 0.0399 | 0.0411 |
Echinococcus multilocularis | protein kinase c iota type | 0.0693 | 0.7161 | 0.7248 |
Loa Loa (eye worm) | AGC/PKA protein kinase | 0.0291 | 0.1219 | 0.1028 |
Echinococcus multilocularis | C2 calcium dependent membrane targeting | 0.0223 | 0.0213 | 0.0215 |
Echinococcus granulosus | phosphorylase b kinase regulatory | 0.0532 | 0.4781 | 0.4839 |
Brugia malayi | protein kinase C3,putative | 0.0468 | 0.3826 | 0.3942 |
Echinococcus granulosus | cAMP dependent protein kinase catalytic subunit | 0.0291 | 0.1219 | 0.1234 |
Echinococcus granulosus | phosphorylase kinase gamma subunit | 0.0318 | 0.161 | 0.1629 |
Echinococcus multilocularis | serine:threonine protein kinase | 0.0239 | 0.0441 | 0.0447 |
Onchocerca volvulus | 0.0649 | 0.6508 | 1 | |
Brugia malayi | phosphorylase B kinase alpha regulatory chain | 0.0532 | 0.4781 | 0.4926 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0291 | 0.1219 | 0.1234 |
Echinococcus multilocularis | cAMP dependent protein kinase catalytic subunit | 0.0291 | 0.1219 | 0.1234 |
Echinococcus multilocularis | cAMP dependent protein kinase catalytic subunit | 0.0291 | 0.1219 | 0.1234 |
Brugia malayi | phosphorylase B kinase beta regulatory chain | 0.0357 | 0.2188 | 0.2254 |
Schistosoma mansoni | phosphorylase B kinase beta kpbb | 0.0357 | 0.2188 | 0.2215 |
Entamoeba histolytica | PH domain containing protein kinase, putative | 0.0426 | 0.321 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0225 | 0.0236 | 0.0024 |
Leishmania major | protein kinase A catalytic subunit isoform 1 | 0.0291 | 0.1219 | 0.5 |
Echinococcus granulosus | cAMP dependent protein kinase catalytic subunit | 0.0291 | 0.1219 | 0.1234 |
Loa Loa (eye worm) | AGC/RSK/MSK protein kinase | 0.0236 | 0.0399 | 0.0191 |
Echinococcus granulosus | protein kinase c iota type | 0.0693 | 0.7161 | 0.7248 |
Trichomonas vaginalis | AGC family protein kinase | 0.0236 | 0.0399 | 0.1659 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0865 | 0.9707 | 0.9825 |
Brugia malayi | Protein kinase domain containing protein | 0.0236 | 0.0399 | 0.0411 |
Loa Loa (eye worm) | CAMK/PHK protein kinase | 0.0318 | 0.161 | 0.1427 |
Schistosoma mansoni | unc-13 (munc13) | 0.0223 | 0.0213 | 0.0215 |
Loa Loa (eye worm) | AGC/PKC/IOTA protein kinase | 0.0487 | 0.4107 | 0.3979 |
Echinococcus multilocularis | enhancer of mRNA decapping protein 4 | 0.0281 | 0.1062 | 0.1075 |
Plasmodium vivax | cAMP-dependent protein kinase catalytic subunit, putative | 0.0291 | 0.1219 | 0.5 |
Brugia malayi | Protein kinase domain containing protein | 0.0318 | 0.161 | 0.1658 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0236 | 0.0399 | 0.0404 |
Echinococcus granulosus | serine:threonine protein kinase N2 | 0.0416 | 0.3068 | 0.3105 |
Schistosoma mansoni | protein kinase C mu | 0.0495 | 0.4235 | 0.4286 |
Trypanosoma brucei | cAMP-dependent protein kinase catalytic subunit 2 | 0.0291 | 0.1219 | 1 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0236 | 0.0399 | 0.0404 |
Echinococcus granulosus | myosin light chain kinase smooth muscle | 0.0239 | 0.0441 | 0.0447 |
Echinococcus granulosus | ribosomal protein s6 kinase beta 1 | 0.0236 | 0.0399 | 0.0404 |
Trichomonas vaginalis | AGC family protein kinase | 0.0246 | 0.0542 | 0.3112 |
Echinococcus multilocularis | Protein kinase C, brain isozyme | 0.0877 | 0.988 | 1 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0291 | 0.1219 | 0.1234 |
Loa Loa (eye worm) | AGC/NDR protein kinase | 0.0236 | 0.0399 | 0.0191 |
Toxoplasma gondii | AGC kinase | 0.0291 | 0.1219 | 1 |
Onchocerca volvulus | 0.0494 | 0.422 | 0.6366 | |
Mycobacterium tuberculosis | Conserved protein | 0.0225 | 0.0236 | 0.5 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0236 | 0.0399 | 0.0404 |
Loa Loa (eye worm) | hypothetical protein | 0.0417 | 0.3084 | 0.2934 |
Loa Loa (eye worm) | hypothetical protein | 0.0649 | 0.6508 | 0.6433 |
Giardia lamblia | Kinase, AGC PKA | 0.0291 | 0.1219 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0649 | 0.6508 | 0.6433 |
Echinococcus multilocularis | cAMP dependent protein kinase catalytic subunit | 0.0291 | 0.1219 | 0.1234 |
Trichomonas vaginalis | AGC family protein kinase | 0.0246 | 0.0542 | 0.3112 |
Echinococcus granulosus | enhancer of mRNA decapping protein 4 | 0.0281 | 0.1062 | 0.1075 |
Loa Loa (eye worm) | hypothetical protein | 0.0286 | 0.1142 | 0.095 |
Trichomonas vaginalis | AGC family protein kinase | 0.0246 | 0.0542 | 0.3112 |
Trypanosoma brucei | protein kinase A catalytic subunit, putative | 0.0291 | 0.1219 | 1 |
Leishmania major | protein kinase A catalytic subunit isoform 2 | 0.0291 | 0.1219 | 0.5 |
Echinococcus multilocularis | myosin light chain kinase, smooth muscle | 0.0239 | 0.0441 | 0.0447 |
Echinococcus granulosus | C2 calcium dependent membrane targeting | 0.0223 | 0.0213 | 0.0215 |
Entamoeba histolytica | protein kinase, putative | 0.0246 | 0.0542 | 0.0508 |
Echinococcus granulosus | Ribosomal protein S6 kinase beta 2 | 0.0236 | 0.0399 | 0.0404 |
Echinococcus multilocularis | telomerase reverse transcriptase subunit | 0.0417 | 0.3084 | 0.3121 |
Entamoeba histolytica | PH domain containing protein kinase, putative | 0.0301 | 0.1362 | 0.3423 |
Loa Loa (eye worm) | AGC/RSK/P70 protein kinase | 0.0236 | 0.0399 | 0.0191 |
Trichomonas vaginalis | AGC family protein kinase | 0.0246 | 0.0542 | 0.3112 |
Loa Loa (eye worm) | hypothetical protein | 0.0286 | 0.1142 | 0.095 |
Echinococcus granulosus | Protein kinase C brain isozyme | 0.0877 | 0.988 | 1 |
Trichomonas vaginalis | AGC family protein kinase | 0.0291 | 0.1219 | 1 |
Trypanosoma cruzi | cAMP-dependent protein kinase catalytic subunit 3 | 0.0291 | 0.1219 | 0.5 |
Trichomonas vaginalis | AGC family protein kinase | 0.0246 | 0.0542 | 0.3112 |
Loa Loa (eye worm) | AGC/AKT protein kinase | 0.0246 | 0.0542 | 0.0337 |
Entamoeba histolytica | protein kinase, putative | 0.0246 | 0.0542 | 0.0508 |
Echinococcus granulosus | RNA directed DNA polymerase | 0.0417 | 0.3084 | 0.3121 |
Echinococcus multilocularis | RNA directed DNA polymerase | 0.0417 | 0.3084 | 0.3121 |
Trypanosoma cruzi | cAMP-dependent protein kinase catalytic subunit 3 | 0.0291 | 0.1219 | 0.5 |
Onchocerca volvulus | Putative phosphorylase b kinase regulatory subunit alpha | 0.0532 | 0.4781 | 0.7257 |
Leishmania major | protein kinase A catalytic subunit | 0.0291 | 0.1219 | 0.5 |
Trichomonas vaginalis | AGC family protein kinase | 0.0236 | 0.0399 | 0.1659 |
Echinococcus granulosus | protein kinase C gamma type | 0.0678 | 0.6945 | 0.7029 |
Loa Loa (eye worm) | AGC/DMPK/GEK protein kinase | 0.0236 | 0.0399 | 0.0191 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0318 | 0.161 | 0.1629 |
Loa Loa (eye worm) | AGC/PKN protein kinase | 0.0236 | 0.0399 | 0.0191 |
Echinococcus granulosus | unc 13 munc13 | 0.0223 | 0.0213 | 0.0215 |
Echinococcus multilocularis | ribosomal protein S6 kinase alpha 3 | 0.0236 | 0.0399 | 0.0404 |
Brugia malayi | cAMP-dependent protein kinase catalytic subunit, putative | 0.0291 | 0.1219 | 0.1256 |
Echinococcus granulosus | NDR protein kinase | 0.0236 | 0.0399 | 0.0404 |
Loa Loa (eye worm) | hypothetical protein | 0.0357 | 0.2188 | 0.2019 |
Loa Loa (eye worm) | hypothetical protein | 0.0494 | 0.422 | 0.4095 |
Echinococcus multilocularis | phosphorylase kinase gamma subunit | 0.0318 | 0.161 | 0.1629 |
Onchocerca volvulus | 0.0286 | 0.1142 | 0.1477 | |
Loa Loa (eye worm) | hypothetical protein | 0.0532 | 0.4781 | 0.4668 |
Echinococcus multilocularis | serine:threonine protein kinase N2 | 0.0236 | 0.0399 | 0.0404 |
Schistosoma mansoni | serine/threonine protein kinase | 0.0236 | 0.0399 | 0.0404 |
Loa Loa (eye worm) | hypothetical protein | 0.0532 | 0.4781 | 0.4668 |
Loa Loa (eye worm) | myosin heavy chain | 0.0225 | 0.0236 | 0.0024 |
Echinococcus granulosus | phosphorylase b kinase regulatory | 0.0357 | 0.2188 | 0.2215 |
Brugia malayi | Phorbol esters/diacylglycerol binding domain | 0.0495 | 0.4235 | 0.4362 |
Trichomonas vaginalis | serine/threonine-protein kinase sgk, putative | 0.0225 | 0.0242 | 0.0062 |
Trichomonas vaginalis | AGC family protein kinase | 0.0236 | 0.0399 | 0.1659 |
Brugia malayi | C1-like domain containing protein | 0.0495 | 0.4235 | 0.4362 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 73300 nM | BindingDB_Patents: Fluorescence Resonance Energy Transfer (FRET) Assay. Potency of test compounds were determined by measurement of their inhibition of BACE1 activity toward a fluorescent substrate. Experiments were performed by reference to the procedure as described in Ermolieff, et al. (Biochemistry 39:12450-12456 (2000), the teachings of which are incorporated hereby in their entirety). Briefly, the recombinant protease unit of BACE1 was prepared from E. coli expression as inclusion bodies, refolded, and purified as described in Lin, et al., (Proc. Nat. Acad. Sci. 97:1456-1460 (2000)). Fluorogenic substrate, MCA-SEVNLDAEFK(DNP)-NH2 (SEQ ID NO:1) was purchased. (M-2485, Bachem Americas, Torrance, Calif.). The substrate was derived from 10 amino acids of the human amyloid precursor protein (APP), with the Swedish variant amino acids at the beta-secretase cleavage site. The terminal amino acid was modified from arginine to lysine to facilitate derivatization with a functional group for detection by autofluorescence. | ChEMBL. | No reference |
IC50 (binding) | = 73300 nM | Fluorescence Resonance Energy Transfer (FRET) Assay | BINDINGDB. | No reference |
IC50 (binding) | = 73300 nM | BindingDB_Patents: Fluorescence Resonance Energy Transfer (FRET) Assay. Potency of test compounds were determined by measurement of their inhibition of BACE1 activity toward a fluorescent substrate. Experiments were performed by reference to the procedure as described in Ermolieff, et al. (Biochemistry 39:12450-12456 (2000), the teachings of which are incorporated hereby in their entirety). Briefly, the recombinant protease unit of BACE1 was prepared from E. coli expression as inclusion bodies, refolded, and purified as described in Lin, et al., (Proc. Nat. Acad. Sci. 97:1456-1460 (2000)). Fluorogenic substrate, MCA-SEVNLDAEFK(DNP)-NH2 (SEQ ID NO:1) was purchased. (M-2485, Bachem Americas, Torrance, Calif.). The substrate was derived from 10 amino acids of the human amyloid precursor protein (APP), with the Swedish variant amino acids at the beta-secretase cleavage site. The terminal amino acid was modified from arginine to lysine to facilitate derivatization with a functional group for detection by autofluorescence. | ChEMBL. | No reference |
IC50 (binding) | = 138000 nM | BindingDB_Patents: Fluorescence Resonance Energy Transfer (FRET) Assay. Potency of test compounds were determined by measurement of their inhibition of BACE1 activity toward a fluorescent substrate. Experiments were performed by reference to the procedure as described in Ermolieff, et al. (Biochemistry 39:12450-12456 (2000), the teachings of which are incorporated hereby in their entirety). Briefly, the recombinant protease unit of BACE1 was prepared from E. coli expression as inclusion bodies, refolded, and purified as described in Lin, et al., (Proc. Nat. Acad. Sci. 97:1456-1460 (2000)). Fluorogenic substrate, MCA-SEVNLDAEFK(DNP)-NH2 (SEQ ID NO:1) was purchased. (M-2485, Bachem Americas, Torrance, Calif.). The substrate was derived from 10 amino acids of the human amyloid precursor protein (APP), with the Swedish variant amino acids at the beta-secretase cleavage site. The terminal amino acid was modified from arginine to lysine to facilitate derivatization with a functional group for detection by autofluorescence. | ChEMBL. | No reference |
IC50 (binding) | = 138000 nM | BindingDB_Patents: Fluorescence Resonance Energy Transfer (FRET) Assay. Potency of test compounds were determined by measurement of their inhibition of BACE1 activity toward a fluorescent substrate. Experiments were performed by reference to the procedure as described in Ermolieff, et al. (Biochemistry 39:12450-12456 (2000), the teachings of which are incorporated hereby in their entirety). Briefly, the recombinant protease unit of BACE1 was prepared from E. coli expression as inclusion bodies, refolded, and purified as described in Lin, et al., (Proc. Nat. Acad. Sci. 97:1456-1460 (2000)). Fluorogenic substrate, MCA-SEVNLDAEFK(DNP)-NH2 (SEQ ID NO:1) was purchased. (M-2485, Bachem Americas, Torrance, Calif.). The substrate was derived from 10 amino acids of the human amyloid precursor protein (APP), with the Swedish variant amino acids at the beta-secretase cleavage site. The terminal amino acid was modified from arginine to lysine to facilitate derivatization with a functional group for detection by autofluorescence. | ChEMBL. | No reference |
IC50 (binding) | = 138000 nM | Fluorescence Resonance Energy Transfer (FRET) Assay | BINDINGDB. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.