Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | Janus kinase 1 | Starlite/ChEMBL | No references |
Homo sapiens | Janus kinase 3 | Starlite/ChEMBL | No references |
Homo sapiens | tyrosine kinase 2 | Starlite/ChEMBL | No references |
Homo sapiens | Janus kinase 2 | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus multilocularis | proto oncogene tyrosine protein kinase LCK | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK/FER protein kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase Src42A | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase Srms | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Src42A | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK/FER protein kinase | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK/ABL protein kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Fyn | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | SRC-1 | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Btk29A | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK/FER protein kinase | 0.0015 | 0.5 | 0.5 |
Brugia malayi | Protein kinase domain containing protein | 0.0015 | 0.5 | 0.5 |
Brugia malayi | hypothetical protein | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase Lyn | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | 3'partial|tyrosine protein kinase Fgr | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | proto oncogene tyrosine protein kinase LCK | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK protein kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | c src tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Srms | 0.0015 | 0.5 | 0.5 |
Onchocerca volvulus | 0.0015 | 0.5 | 0.5 | |
Onchocerca volvulus | 0.0015 | 0.5 | 0.5 | |
Loa Loa (eye worm) | TK/FER protein kinase | 0.0015 | 0.5 | 0.5 |
Schistosoma mansoni | tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase shark | 0.0015 | 0.5 | 0.5 |
Onchocerca volvulus | 0.0015 | 0.5 | 0.5 | |
Echinococcus multilocularis | tyrosine protein kinase Blk | 0.0015 | 0.5 | 0.5 |
Onchocerca volvulus | 0.0015 | 0.5 | 0.5 | |
Echinococcus granulosus | tyrosine protein kinase Btk29A | 0.0015 | 0.5 | 0.5 |
Brugia malayi | SH2 domain containing protein | 0.0015 | 0.5 | 0.5 |
Schistosoma mansoni | tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase ABL1 | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | c src tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Fgr | 0.0015 | 0.5 | 0.5 |
Brugia malayi | Protein kinase domain containing protein | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase Fps85D | 0.0015 | 0.5 | 0.5 |
Schistosoma mansoni | tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK/FER protein kinase | 0.0015 | 0.5 | 0.5 |
Brugia malayi | protein-tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK/FER protein kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase shark | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK/FER protein kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase Blk | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK/FER protein kinase | 0.0015 | 0.5 | 0.5 |
Schistosoma mansoni | tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase Fyn | 0.0015 | 0.5 | 0.5 |
Schistosoma mansoni | tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Fyn | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK/FER protein kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Fyn | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase ABL1 | 0.0015 | 0.5 | 0.5 |
Brugia malayi | Protein kinase domain containing protein | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase Src64B | 0.0015 | 0.5 | 0.5 |
Onchocerca volvulus | 0.0015 | 0.5 | 0.5 | |
Echinococcus granulosus | tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase lyn tyrosine protein kinase blk | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase Fyn | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine kinase|tyrosine protein kinase Fyn | 0.0015 | 0.5 | 0.5 |
Brugia malayi | Tyrosine-protein kinase abl-1 | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Fps85D | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Src64B | 0.0015 | 0.5 | 0.5 |
Entamoeba histolytica | SH2-protein kinase domain containing protein | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Abl | 0.0015 | 0.5 | 0.5 |
Schistosoma mansoni | proto-oncogene tyrosine-protein kinase src | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0015 | 0.5 | 0.5 |
Echinococcus granulosus | tyrosine protein kinase Fyn | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase lyn lyn a protein tyrosine kinase lymphocyte specific protein tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Loa Loa (eye worm) | TK/FER protein kinase | 0.0015 | 0.5 | 0.5 |
Echinococcus multilocularis | tyrosine protein kinase Fyn | 0.0015 | 0.5 | 0.5 |
Brugia malayi | Protein kinase domain containing protein | 0.0015 | 0.5 | 0.5 |
Brugia malayi | SRC-1 | 0.0015 | 0.5 | 0.5 |
Schistosoma mansoni | tyrosine kinase | 0.0015 | 0.5 | 0.5 |
Brugia malayi | SH2 domain containing protein | 0.0015 | 0.5 | 0.5 |
Onchocerca volvulus | 0.0015 | 0.5 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
Ki (binding) | = 1.5 nM | Inhibition Assay | BINDINGDB. | No reference |
Ki (binding) | = 4.8 nM | Inhibition Assay | BINDINGDB. | No reference |
Ki (binding) | = 6.6 nM | BindingDB_Patents: Inhibition Assay. The activity of the isolated JAK1, JAK2 or TYK2 kinase domain was measured by monitoring phosphorylation of a peptide derived from JAK3 (Val-Ala-Leu-Val-Asp-Gly-Tyr-Phe-Arg-Leu-Thr-Thr) fluorescently labeled on the N-terminus with 5-carboxyfluorescein using the Caliper LabChip technology (Caliper Life Sciences, Hopkinton, Mass.). To determine the inhibition constants (Ki) of Examples 1-508, compounds were diluted serially in DMSO and added to 50 uL kinase reactions containing 1.5 nM JAK1, 0.2 nM purified JAK2 or 1 nM purified TYK2 enzyme, 100 mM Hepes pH7.2, 0.015% Brij-35, 1.5 uM peptide substrate, 25 uM ATP, 10 mM MgCl2, 4 mM DTT at a final DMSO concentration of 2%. Reactions were incubated at 22° C. in 384-well polypropylene microtiter plates for 30 minutes and then stopped by addition of 25 uL of an EDTA containing solution (100 mM Hepes pH 7.2, 0.015% Brij-35, 150 mM EDTA), resulting in a final EDTA concentration of 50 mM. | ChEMBL. | No reference |
Ki (binding) | = 53 nM | BindingDB_Patents: Inhibition Assay. The activity of the isolated JAK3 kinase domain was measured by monitoring phosphorylation of a peptide derived from JAK3 (Leu-Pro-Leu-Asp-Lys-Asp-Tyr-Tyr-Val-Val-Arg) fluorescently labeled on the N-terminus with 5-carboxyfluorescein using the Caliper LabChip technology (Caliper Life Sciences, Hopkinton, Mass.). To determine the inhibition constants (Ki) of Examples 1-508, compounds were diluted serially in DMSO and added to 50 uL kinase reactions containing 5 nM purified JAK3 enzyme, 100 mM Hepes pH7.2, 0.015% Brij-35, 1.5 uM peptide substrate, 5 uM ATP, 10 mM MgCl2, 4 mM DTT at a final DMSO concentration of 2%. Reactions were incubated at 22° C. in 384-well polypropylene microtiter plates for 30 minutes and then stopped by addition of 25 uL of an EDTA containing solution (100 mM Hepes pH 7.2, 0.015% Brij-35, 150 mM EDTA), resulting in a final EDTA concentration of 50 mM. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.