Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | isocitrate dehydrogenase 1 (NADP+), soluble | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Schistosoma mansoni | serine/threonine-protein kinase | 0.0025 | 0.0138 | 0.0138 |
Plasmodium falciparum | RAC-beta serine/threonine protein kinase | 0.0023 | 0.0114 | 0.004 |
Trichomonas vaginalis | conserved hypothetical protein | 0.0468 | 0.4693 | 1 |
Echinococcus multilocularis | thymidylate synthase | 0.0984 | 1 | 1 |
Echinococcus multilocularis | NADP dependent isocitrate dehydrogenase | 0.0019 | 0.0075 | 0.0075 |
Schistosoma mansoni | serine/threonine-protein kinase | 0.0025 | 0.0138 | 0.0138 |
Trypanosoma cruzi | rac serine-threonine kinase, putative | 0.0023 | 0.0114 | 0.004 |
Echinococcus multilocularis | nervana 2 | 0.0023 | 0.0112 | 0.0112 |
Trichomonas vaginalis | AGC family protein kinase | 0.0037 | 0.0257 | 0.0547 |
Mycobacterium leprae | PROBABLE THYMIDYLATE SYNTHASE THYA (TS) (TSASE) | 0.0984 | 1 | 0.5 |
Plasmodium falciparum | bifunctional dihydrofolate reductase-thymidylate synthase | 0.0984 | 1 | 1 |
Loa Loa (eye worm) | thymidylate synthase | 0.0984 | 1 | 1 |
Giardia lamblia | Kinase, AGC PKA | 0.0023 | 0.0114 | 0.5 |
Echinococcus multilocularis | isocitrate dehydrogenase 2 (NADP+) | 0.0019 | 0.0075 | 0.0075 |
Echinococcus granulosus | thymidylate synthase | 0.0984 | 1 | 1 |
Entamoeba histolytica | protein kinase, putative | 0.0037 | 0.0257 | 1 |
Trichomonas vaginalis | AGC family protein kinase | 0.0037 | 0.0257 | 0.0547 |
Trichomonas vaginalis | AGC family protein kinase | 0.0037 | 0.0257 | 0.0547 |
Echinococcus granulosus | serine threonine protein kinase nrc | 0.0023 | 0.0114 | 0.0114 |
Echinococcus granulosus | serine/threonine protein kinase | 0.0025 | 0.0138 | 0.0138 |
Onchocerca volvulus | 0.0984 | 1 | 0.5 | |
Trichomonas vaginalis | AGC family protein kinase | 0.0037 | 0.0257 | 0.0547 |
Trypanosoma brucei | rac serine-threonine kinase, putative | 0.0037 | 0.0257 | 0.0184 |
Brugia malayi | isocitrate dehydrogenase | 0.0019 | 0.0075 | 0.0075 |
Trichomonas vaginalis | AGC family protein kinase | 0.0037 | 0.0257 | 0.0547 |
Echinococcus granulosus | Glutaredoxin protein 5 | 0.0023 | 0.0112 | 0.0112 |
Echinococcus granulosus | nervana 2 | 0.0023 | 0.0112 | 0.0112 |
Entamoeba histolytica | PH domain containing protein kinase, putative | 0.0025 | 0.0138 | 0.5363 |
Trypanosoma brucei | dihydrofolate reductase-thymidylate synthase | 0.0984 | 1 | 1 |
Entamoeba histolytica | protein kinase, putative | 0.0035 | 0.0233 | 0.909 |
Loa Loa (eye worm) | AGC/AKT protein kinase | 0.0037 | 0.0257 | 0.0257 |
Trichomonas vaginalis | AGC family protein kinase | 0.0037 | 0.0257 | 0.0547 |
Trypanosoma cruzi | dihydrofolate reductase-thymidylate synthase, putative | 0.0468 | 0.4693 | 0.4654 |
Entamoeba histolytica | PH domain containing protein kinase, putative | 0.0025 | 0.0138 | 0.5363 |
Trichomonas vaginalis | AGC family protein kinase | 0.0014 | 0.0023 | 0.005 |
Mycobacterium tuberculosis | Probable thymidylate synthase ThyA (ts) (TSASE) | 0.0984 | 1 | 1 |
Brugia malayi | p70 ribosomal S6 kinase beta | 0.0035 | 0.0233 | 0.0233 |
Echinococcus granulosus | calcium:calmodulin dependent protein kinase | 0.0023 | 0.0114 | 0.0114 |
Brugia malayi | Isocitrate dehydrogenase | 0.0019 | 0.0075 | 0.0075 |
Echinococcus granulosus | NADP dependent isocitrate dehydrogenase | 0.0019 | 0.0075 | 0.0075 |
Echinococcus multilocularis | Glutaredoxin protein 5 | 0.0023 | 0.0112 | 0.0112 |
Plasmodium vivax | rac-beta serine/threonine protein kinase, putative | 0.0023 | 0.0114 | 0.004 |
Toxoplasma gondii | bifunctional dihydrofolate reductase-thymidylate synthase | 0.0984 | 1 | 1 |
Echinococcus multilocularis | serine threonine protein kinase nrc serine threonine protein kinase gad | 0.0023 | 0.0114 | 0.0114 |
Mycobacterium tuberculosis | Hypothetical protein | 0.0468 | 0.4693 | 0.4654 |
Loa Loa (eye worm) | isocitrate dehydrogenase | 0.0019 | 0.0075 | 0.0075 |
Echinococcus multilocularis | NADP dependent isocitrate dehydrogenase | 0.0019 | 0.0075 | 0.0075 |
Brugia malayi | hypothetical protein | 0.0468 | 0.4693 | 0.4693 |
Schistosoma mansoni | bifunctional dihydrofolate reductase-thymidylate synthase | 0.0984 | 1 | 1 |
Mycobacterium ulcerans | thymidylate synthase | 0.0984 | 1 | 0.5 |
Trypanosoma cruzi | rac serine-threonine kinase, putative | 0.0025 | 0.0138 | 0.0064 |
Trichomonas vaginalis | AGC family protein kinase | 0.0037 | 0.0257 | 0.0547 |
Toxoplasma gondii | AGC kinase | 0.0035 | 0.0233 | 0.016 |
Entamoeba histolytica | protein kinase 2, putative | 0.0023 | 0.0114 | 0.4453 |
Echinococcus multilocularis | rac serine:threonine kinase | 0.0025 | 0.0138 | 0.0138 |
Echinococcus multilocularis | isocitrate dehydrogenase | 0.0019 | 0.0075 | 0.0075 |
Loa Loa (eye worm) | AGC/RSK/P70 protein kinase | 0.0035 | 0.0233 | 0.0233 |
Trypanosoma cruzi | dihydrofolate reductase-thymidylate synthase | 0.0984 | 1 | 1 |
Plasmodium vivax | bifunctional dihydrofolate reductase-thymidylate synthase, putative | 0.0984 | 1 | 1 |
Trypanosoma cruzi | Protein kinase B | 0.0025 | 0.0138 | 0.0064 |
Echinococcus multilocularis | nervana 2 | 0.0023 | 0.0112 | 0.0112 |
Brugia malayi | Protein kinase domain containing protein | 0.0037 | 0.0257 | 0.0257 |
Schistosoma mansoni | NADP-specific isocitrate dehydrogenase | 0.0019 | 0.0075 | 0.0075 |
Leishmania major | dihydrofolate reductase-thymidylate synthase | 0.0984 | 1 | 1 |
Entamoeba histolytica | protein kinase, putative | 0.0037 | 0.0257 | 1 |
Echinococcus granulosus | sodium:potassium dependent atpase beta subunit | 0.0023 | 0.0112 | 0.0112 |
Echinococcus multilocularis | NADP dependent isocitrate dehydrogenase | 0.0019 | 0.0075 | 0.0075 |
Echinococcus multilocularis | sodium:potassium dependent atpase beta subunit | 0.0023 | 0.0112 | 0.0112 |
Echinococcus granulosus | nervana 2 | 0.0023 | 0.0112 | 0.0112 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 7481 nM | BindingDB_Patents: LC-MS Biochemical Assay. Mutant IDH1 R132H catalytic activity was monitored using the quantitative liquid chromatography/mass spectrometry (LC-MS) detection of 2-HG, a product of the NADPH-dependent alpha-KG reduction reaction.More specifically, the biochemical reactions were performed at room temperature in 384-well Greiner flat-bottom plates (Costar, Cat. No. 781201) using a final reaction volume of 30 uL and the following assay buffer conditions: 50 mM HEPES pH 7.4, 10 mM MgCl2, 50 mM KCl, 1 mM DTT, 0.02% BSA, 5 uM NADPH and 100 uM alpha-KG.The final reaction mixture contained 3.3% DMSO and inhibitors with concentrations ranging 0.02-50 uM. The IDH1 enzyme was used at a final concentration of 0.25 nM. Following 45 minutes incubation, the reaction mixtures were quenched by the addition of 10 uL of 16% formic acid containing 800 nM of 5-carbon labeled 13C-2-HG). The protein was then precipitated by the addition of 2.5 volumes of acetonitrile followed by centrifugation (3000xg, 20 minutes). | ChEMBL. | No reference |
IC50 (binding) | = 7481 nM | BindingDB_Patents: LC-MS Biochemical Assay. Mutant IDH1 R132H catalytic activity was monitored using the quantitative liquid chromatography/mass spectrometry (LC-MS) detection of 2-HG, a product of the NADPH-dependent alpha-KG reduction reaction.More specifically, the biochemical reactions were performed at room temperature in 384-well Greiner flat-bottom plates (Costar, Cat. No. 781201) using a final reaction volume of 30 uL and the following assay buffer conditions: 50 mM HEPES pH 7.4, 10 mM MgCl2, 50 mM KCl, 1 mM DTT, 0.02% BSA, 5 uM NADPH and 100 uM alpha-KG.The final reaction mixture contained 3.3% DMSO and inhibitors with concentrations ranging 0.02-50 uM. The IDH1 enzyme was used at a final concentration of 0.25 nM. Following 45 minutes incubation, the reaction mixtures were quenched by the addition of 10 uL of 16% formic acid containing 800 nM of 5-carbon labeled 13C-2-HG). The protein was then precipitated by the addition of 2.5 volumes of acetonitrile followed by centrifugation (3000xg, 20 minutes). | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.