Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | chemokine (C-X-C motif) receptor 2 | Starlite/ChEMBL | References |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Schistosoma mansoni | purine nucleoside phosphorylase | 0.0129 | 1 | 0.5 |
Schistosoma mansoni | purine nucleoside phosphorylase | 0.0129 | 1 | 0.5 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Mycobacterium ulcerans | purine nucleoside phosphorylase | 0.0129 | 1 | 0.5 |
Mycobacterium tuberculosis | Probable purine nucleoside phosphorylase DeoD (inosine phosphorylase) (PNP) | 0.0129 | 1 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0102 | 0 | 0.5 |
Giardia lamblia | Purine nucleoside phosphorylase lateral transfer candidate | 0.0129 | 1 | 0.5 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Onchocerca volvulus | Purine nucleoside phosphorylase homolog | 0.0129 | 1 | 0.5 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Mycobacterium leprae | Probable purine nucleoside phosphorylase DeoD (INOSINE PHOSPHORYLASE) (PNP) | 0.0129 | 1 | 0.5 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Echinococcus multilocularis | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Echinococcus granulosus | purine nucleoside phosphorylase | 0.0129 | 1 | 1 |
Trichomonas vaginalis | purine nucleoside phosphorylase I, putative | 0.0129 | 1 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 3900 nM | BindingDB_Patents: In Vitro Inhibition Assay. An in vitro assay showed inhibition of CXCR2-mediated intracellular calcium release. Briefly, human neutrophils were suspended in HBSS- (without Ca2+ and Mg2+) containing 10 mM HEPES and FLIPR Calcium 3 dye (3.1x107 cells in total volume 1.7 mL). Cells were aliquoted (200 uL of the cell suspension per tube, 8 tubes total) and 2 uL of the designated compound (with appropriate dilutions) were added to each of 6 tubes. As controls, 2 uL of DMSO (1% final concentration) were added to 2 other tubes. Cells were incubated for 30 min at 37 C. After dye loading, tubes were centrifuged at 6,000 rpm for 1 min, supernatant was removed and the cell pellet was re-suspended in 200 uL of HBSS+ (with Ca2+ and Mg2+) containing 10 mM HEPES. The test compound or DMSO (control) was added again at the same concentrations that were used during cell loading. The cell suspension was aliquoted into a 96-well Reading Plate (Corning) in a volume of 90 uL (105 cells/well). | ChEMBL. | No reference |
IC50 (binding) | = 3900 nM | BindingDB_Patents: In Vitro Inhibition Assay. An in vitro assay showed inhibition of CXCR2-mediated intracellular calcium release. Briefly, human neutrophils were suspended in HBSS- (without Ca2+ and Mg2+) containing 10 mM HEPES and FLIPR Calcium 3 dye (3.1x107 cells in total volume 1.7 mL). Cells were aliquoted (200 uL of the cell suspension per tube, 8 tubes total) and 2 uL of the designated compound (with appropriate dilutions) were added to each of 6 tubes. As controls, 2 uL of DMSO (1% final concentration) were added to 2 other tubes. Cells were incubated for 30 min at 37 C. After dye loading, tubes were centrifuged at 6,000 rpm for 1 min, supernatant was removed and the cell pellet was re-suspended in 200 uL of HBSS+ (with Ca2+ and Mg2+) containing 10 mM HEPES. The test compound or DMSO (control) was added again at the same concentrations that were used during cell loading. The cell suspension was aliquoted into a 96-well Reading Plate (Corning) in a volume of 90 uL (105 cells/well). | ChEMBL. | No reference |
IC50 (binding) | = 3.9 uM | Antagonist activity at CXCR2 in human PMNs assessed as inhibition of CXCL1-induced intracellular Ca2+ release by fluorescence based calcium flux assay | ChEMBL. | 25254640 |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.
1 literature reference was collected for this gene.