Detailed view for PF3D7_0808200

Basic information

TDR Targets ID: 1041
Plasmodium falciparum, plasmepsin X
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 5.2173 | Length (AA): 573 | MW (Da): 65114 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00026   Eukaryotic aspartyl protease

Gene Ontology

Mouse over links to read term descriptions.
GO:0004190   aspartic-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
192 571 1miq (A) 78 327 27.00 0 1 0.98 -0.96
213 512 1qdm (A) 9 29 31.00 0 1 0.87 0.18
234 480 1b5f (A) 0 238 29.00 0 1 0.78 -0.44
239 567 1qrp (E) 5 323 34.00 0 1 1.06 -1.28
247 570 1hrn (A) 13 326 27.00 0 1 0.89 -1.32
113 236 3na7 (A) 31 161 21.00 0.65 0.01 0.500305 -1.31
194 571 3qvc (A) 86 327 30.00 0 1 0.926586 -0.25
194 570 3psg (A) 3 326 31.00 0 1 1.05494 -0.77
240 324 4od9 (A) 7 91 40.00 0.000000000061 0.92 0.575242 0.17
340 569 1am5 (A) 97 325 22.00 0.00000000055 1 0.646396 0.1
485 570 1b5f (B) 246 326 29.00 0.000043 0.35 0.267087 0.68

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, merozoite, sporozoite, late schizont, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, gametocyte, early ring, early schizont, Oocyst, Ring, Female Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 16 hs, Male Gametocyte. Otto TD Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs. Otto TD
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_133546)

Species Accession Gene Product
Babesia bovis BBOV_IV007890   aspartyl protease, putative
Cryptosporidium parvum cgd6_660   secreted pepsinogen like aspartyl protease having a signal peptide
Homo sapiens 643834   pepsinogen 3, group I (pepsinogen A)
Homo sapiens 101929842   pepsin A-3-like
Homo sapiens 643847   pepsinogen 4, group I (pepsinogen A)
Homo sapiens ENSG00000256713   pepsinogen 5, group I (pepsinogen A)
Mus musculus ENSMUSG00000046213   chymosin
Neospora caninum NCLIV_063340   hypothetical protein
Plasmodium berghei PBANKA_1222500   plasmepsin X
Plasmodium berghei PBANKA_1014500   plasmepsin IX, putative
Plasmodium falciparum PF3D7_0808200   plasmepsin X
Plasmodium falciparum PF3D7_1430200   plasmepsin IX
Plasmodium knowlesi PKNH_0110500   eukaryotic aspartyl protease, putative
Plasmodium knowlesi PKNH_1328500   aspartyl protease, putative
Plasmodium vivax PVX_088125   aspartyl protease, putative
Plasmodium vivax PVX_085030   aspartyl protease, putative
Plasmodium yoelii PY01268   pepsinogen A-related
Plasmodium yoelii PY06692   Eukaryotic aspartyl protease, putative
Toxoplasma gondii TGME49_246550   aspartyl protease ASP3
Theileria parva TP03_0307   hypothetical protein

Essentiality

PF3D7_0808200 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1014500 Plasmodium berghei Essential plasmo
PBANKA_1222500 Plasmodium berghei Essential plasmo
TGME49_246550 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens pepsinogen 5, group I (pepsinogen A) Compounds References
Sus scrofa Pepsin A Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bos taurus Cathepsin D 390 aa 28.8% 351 aa Compounds References
Callithrix jacchus Renin 400 aa 26.6% 338 aa Compounds References
Oryctolagus cuniculus Renin 280 aa 25.9% 290 aa Compounds References
Rattus norvegicus Pepsinogen C 392 aa 31.6% 326 aa Compounds References
Schistosoma mansoni cathepsin D (A01 family) 430 aa 31.8% 349 aa Compounds References
Schistosoma mansoni cathepsin D (A01 family) 428 aa 31.8% 349 aa Compounds References
Macaca fascicularis Renin 406 aa 26.7% 352 aa Compounds References
Macaca mulatta Renin 406 aa 26.8% 351 aa Compounds References
Rattus norvegicus Renin 402 aa 29.8% 332 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0674 0.362 0.504
0.1815 0.2617 1
0.1952 0.2915 1
0.1932 0.2718 1
0.0295 0.2875 1
0.0374 0.2743 0.2704
0.0726 0.2726 1
0.0507 0.2937 0.848
0.0481 0.3678 0.3318
0.233 0.3236 1
0.0485 0.2727 1
0.123 0.5668 0.7703
0.0437 0.2835 1
0.1544 0.3525 1
0.1692 0.2898 1
0.0021 1 0.5
0.137 0.2634 1
0.0474 0.587 1
0.2143 0.381 1
0.0427 0.369 1
0.1019 0.2828 1
0.1362 0.2891 1
0.0739 0.6497 1
0.0306 0.4069 1
0.1853 0.278 1
0.2072 0.3007 1
0.034 0.3396 1
0.0408 0.2732 0.847
0.0103 0.2611 0
0.1799 0.307 1
0.0228 0.2545 1
0.168 0.2913 1
0.0428 0.3604 1
0.2338 0.3281 1
0.0487 0.5025 1
0.0295 0.7362 1
0.044 0.2931 1
0.1591 0.306 1
0.2118 0.3646 1
0.0228 0.2545 1
0.2072 0.3007 1
0.1177 0.3065 1
0.0483 0.538 1
0.1483 0.2711 0.9458
0.0228 0.2758 1
0.0295 0.2875 1
0.0625 0.2528 0.8245
0.0285 0.3643 0.6591
0.1394 0.2637 1
0.1057 0.4236 0.781
0.1885 0.3481 1
0.1775 0.332 1
0.2307 0.3301 1
0.0298 0.4616 1
0.0516 0.3055 1
0.1941 0.3449 1
0.1941 0.3451 1
0.0617 0.4441 1
0.0414 0.8539 0.5
0.0613 0.3064 0.8567
0.0286 0.5159 1
0.1528 0.2938 0.9262
0.0244 0.2755 1
0.0282 0.4377 1
0.0419 0.3274 1
0.05 0.2891 1
0.0021 0.443 0.5
0.1941 0.3451 1
0.0289 0.2839 1
0.0617 0.4441 1
0.1656 0.3163 0.9218
0.0506 0.4215 0.85
0.1952 0.2915 1
0.1369 0.2917 1
0.2338 0.3281 1
0.2033 0.3513 1
0.0388 0.2709 1
0.0021 0.4249 0.5
0.0433 0.535 1
0.0102 0.3299 0.341
0.0352 0.3026 0.4452
0.0021 1 0.5
0.1831 0.3366 1
0.1369 0.2917 1
0.0271 0.4762 1
0.2338 0.3281 1
0.03 0.7448 1
0.0339 0.2726 1
0.0103 0.3898 1
0.0181 0.3592 0
0.1483 0.2711 0.9458
0.0323 0.3278 0.9363
0.1307 0.2533 1
0.1064 0.269 0.8302
0.1146 0.4454 1
0.0711 0.2808 1
0.1019 0.2828 1
0.014 0.2689 1
0.03 0.363 1
0.0253 0.2785 0.7301
0.1544 0.3525 1
0.037 0.2542 0.8197
0.0304 0.2518 0.7938
0.0101 0.2815 1
0.1483 0.2711 0.9458
0.2203 0.4051 1
0.2063 0.3773 1
0.0619 0.3666 1
0.0617 0.4441 1
0.1805 0.3305 1
0.0278 0.327 1
0.0617 0.4441 1
0.0195 0.2824 0.54
0.0674 0.3632 0.5054
0.0126 0.3912 1
0.0322 0.5618 1
0.0736 0.5247 1
0.2379 0.4175 1
0.0457 0.5286 1
0.0874 0.6406 0.6497
0.1412 0.275 1
0.0301 0.3656 1
0.2072 0.3007 1
0.1279 0.2509 1
0.1352 0.2613 1
0.1669 0.2935 1
0.0963 0.5975 1
0.0021 1 0.5
0.1057 0.4236 0.781
0.0402 0.418 1
0.0112 0.8509 1
0.044 0.2931 1
0.2063 0.2939 1
0.002 0.5 0.5
0.0521 0.2928 0.4903
0.0091 0.2886 1
0.1019 0.2828 1
0.0125 0.2612 1
0.1387 0.2714 0.8554
0.1685 0.3143 1
0.0674 0.362 0.504
0.0021 0.7491 0.5
0.1483 0.2711 0.9458
0.0688 0.2679 0.5289
0.0199 0.2803 0
0.0087 0.2691 1
0.1692 0.2898 1
0.0298 0.4139 1
0.1279 0.2521 0.9857
0.1621 0.2511 1
0.0619 0.3666 1
0.1019 0.2828 1
0.1373 0.2643 1
0.2093 0.3777 1
0.1656 0.3163 0.9218
0.014 0.2785 1
0.0123 0.2731 1
0.1378 0.3102 1
0.0228 0.2545 1
0.2172 0.3886 1
0.0299 0.2567 0.9246
0.0309 0.256 0.5059
0.0417 0.3353 0.4626
0.0106 0.5 0.5
0.0616 0.2754 0.8874
0.2025 0.3675 1
0.2338 0.3281 1
0.0112 0.8536 0.5
0.0221 0.3583 1
0.1654 0.3623 1
0.1177 0.3065 1
0.0392 0.5179 0.9171
0.0619 0.3666 1
0.2072 0.3007 1
0.2118 0.3646 1
0.1931 0.3556 1
0.1828 0.3412 1
0.0478 0.6618 1
0.1952 0.2915 1
0.0108 0.5127 0.2693
0.049 0.3576 1
0.0392 0.5179 0.9171
0.0194 0.2631 1
0.0294 0.5253 1
0.1057 0.4236 0.781
0.2072 0.3007 1
0.0702 0.3709 1
0.1588 0.2993 1
0.0278 0.3056 1
0.0307 0.263 1
0.0102 0.3805 1
0.1831 0.3366 1
0.1831 0.3366 1
0.0068 1 0.5
0.1177 0.3065 1
0.1474 0.2759 1
0.2036 0.3702 1
0.0516 0.3055 1
0.0389 0.2556 0.9067
0.0226 0.4352 0.7399
0.2307 0.3301 1
0.1493 0.2812 1
0.1034 0.2723 1
0.0745 0.3328 1
0.1014 0.2925 1
0.0775 0.2539 0.838
0.2191 0.4253 1
0.1994 0.3474 1
0.0387 0.3825 1
0.149 0.2579 1
0.2338 0.3281 1
0.0021 1 0.5
0.02 0.4636 1
0.123 0.5668 0.7703
0.1935 0.2793 1
0.0205 0.4058 0.9061
0.0242 0.2691 1
0.0491 0.2904 1
0.0655 0.2805 0.5732
0.039 0.2679 0.8077
0.1377 0.2642 1
0.1794 0.4025 1
0.1762 0.3354 1
0.2143 0.381 1
0.1461 0.2726 1
0.1654 0.3623 1
0.0133 0.2691 1
0.061 0.3041 0.7595
0.0021 1 0.5
0.1506 0.4174 1
0.0112 0.5195 0.5
0.0765 0.5842 0.5822
0.1426 0.2616 1
0.1246 0.2643 1
0.157 0.3435 1
0.2338 0.3281 1
0.0021 1 0.5
0.2143 0.381 1
0.137 0.2634 1
0.0433 0.535 1
0.0527 0.6933 1
0.233 0.3236 1
0.2545 0.3823 1
0.0343 0.351 1
0.0021 1 0.5
0.1483 0.2711 0.9458
0.047 0.319 1
0.1961 0.353 1
0.2059 0.4038 1
0.2145 0.3807 1
0.0336 0.4821 1
0.014 0.285 1
0.0655 0.2805 0.5732
0.0091 0.522 1
0.0021 1 0.5
0.1467 0.2836 1
0.1008 0.3121 1
0.0228 0.2545 1
0.0426831 0.259534 1
0.0327 0.2509 0.9459
0.2203 0.4051 1
0.0112 0.3524 1
0.0113 0.2638 1
0.1959 0.3705 1
0.1702 0.2908 1
0.1938 0.2729 1
0.1361 0.2931 1
0.0427 0.369 1
0.0191 0.3331 0.4047
0.0484 0.3541 0.3541
0.014 0.2769 1
0.021 0.2775 0.9074
0.1853 0.278 1
0.0298 0.3721 1
0.1054 0.2728 1
0.0402 0.4634 1
0.0112 0.8454 0.5
0.1941 0.3451 1
0.0021 1 0.5
0.03 0.363 1
0.1656 0.3163 0.9218
0.0126 0.3912 1
0.1078 0.61 1
0.0354 0.3164 1
0.0521 0.2928 0.4903
0.0707 0.4532 1
0.1394 0.2674 1
0.1941 0.3451 1
0.1558 0.3016 1
0.0106 0.5 0.5
0.2063 0.3773 1
0.1078 0.61 1
0.0285 0.3588 0.6341
0.1709 0.2526 1
0.0334 0.4177 1
0.014 0.2689 1
0.0435 0.5315 1
0.1929 0.338 1
0.2203 0.4051 1

Assayability

Assay information

  • Assay for Pepsinogen (3.4.23.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

9 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_0808200 (Plasmodium falciparum), plasmepsin X
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