Detailed view for Tb927.8.2380

Basic information

TDR Targets ID: 10630
Trypanosoma brucei, ABC transporter, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1936 | Length (AA): 1158 | MW (Da): 126931 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 9

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00005   ABC transporter
PF01061   ABC-2 type transporter

Gene Ontology

Mouse over links to read term descriptions.
GO:0042626   ATPase activity, coupled to transmembrane movement of substances  
GO:0009898   internal side of plasma membrane  
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0017111   nucleoside-triphosphatase activity  
GO:0016887   ATPase activity  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
470 803 5ko2 (A) 332 690 27.00 0 1 0.476128 0.58
476 774 5kpj (A) 973 1247 25.00 0 1 0.456504 0.27
505 779 4s0f (A) 467 702 33.00 0 0.99 0.358178 0.58
534 762 3tif (A) 2 225 27.00 0 1 0.578455 -0.65
538 1155 5do7 (B) 35 667 28.00 0 1 0.701379 0.58
547 741 4mrs (A) 371 546 39.00 0.0000000000063 1 0.426094 0.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128747)

Species Accession Gene Product
Arabidopsis thaliana AT3G52310   ABC transporter G family member 27
Arabidopsis thaliana AT5G06530   ABC transporter G family member 22
Candida albicans CaO19.8090   ATP-binding cassette protein similar to S. cerevisiae ADP1 (YCR011C)
Candida albicans CaO19.459   ATP-binding cassette protein similar to S. cerevisiae ADP1 (YCR011C)
Caenorhabditis elegans CELE_F02E11.1   Protein WHT-4
Cryptosporidium parvum cgd6_5350   putative ABC transporter protein
Dictyostelium discoideum DDB_G0282103   ABC transporter G family protein
Drosophila melanogaster Dmel_CG3164   CG3164 gene product from transcript CG3164-RA
Drosophila melanogaster Dmel_CG3327   Early gene at 23
Drosophila melanogaster Dmel_CG2969   ABC transporter expressed in trachea
Leishmania braziliensis LbrM.23.0420   ABC transporter-like protein
Leishmania donovani LdBPK_363040.1   ATP-binding cassette protein subfamily G, member 6, putative
Leishmania donovani LdBPK_230430.1   ATP-binding cassette protein subfamily G, member 5, putative
Leishmania infantum LinJ.23.0430   ATP-binding cassette protein subfamily G, member 5, putative
Leishmania infantum LinJ.36.3040   ATP-binding cassette protein subfamily G, member 6, putative
Leishmania major LmjF.23.0380   ATP-binding cassette protein subfamily G, member 5, putative
Leishmania mexicana LmxM.36.2890   ATP-binding cassette protein, putative
Leishmania mexicana LmxM.23.0380   ABC transporter-like protein
Loa Loa (eye worm) LOAG_13375   hypothetical protein
Loa Loa (eye worm) LOAG_12792   hypothetical protein
Loa Loa (eye worm) LOAG_15135   hypothetical protein
Oryza sativa 9272142   Os07g0191600
Oryza sativa 4331674   Os03g0157400
Saccharomyces cerevisiae YCR011C   putative ATP-dependent permease ADP1
Schmidtea mediterranea mk4.018420.00   Putative abc transporter
Trypanosoma brucei gambiense Tbg972.8.1830   ABC transporter, putative
Trypanosoma brucei Tb927.8.2380   ABC transporter, putative
Trypanosoma cruzi TcCLB.507241.39   ABC transporter, putative
Trypanosoma cruzi TcCLB.504425.70   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.509331.200   ABC transporter, putative
Toxoplasma gondii TGME49_290585   hypothetical protein
Toxoplasma gondii TGME49_290580   ATP-binding cassette G family transporter ABCG89

Essentiality

Tb927.8.2380 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.2380 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.2380 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.2380 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.2380 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_290580 Toxoplasma gondii Essentiality uncertain sidik
TGME49_290585 Toxoplasma gondii Essentiality uncertain sidik
TGME49_290580 Toxoplasma gondii Probably non-essential sidik
TGME49_290585 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.8.2380 (Trypanosoma brucei), ABC transporter, putative
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