Detailed view for Tb927.11.2230

Basic information

TDR Targets ID: 10869
Trypanosoma brucei, carnitine O-acetyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.0024 | Length (AA): 737 | MW (Da): 82427 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00755   Choline/Carnitine o-acyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0004092   carnitine O-acetyltransferase activity  
GO:0016746   transferase activity, transferring acyl groups  
GO:0016021   integral to membrane  
GO:0008415   acyltransferase activity  
GO:0006091   generation of precursor metabolites and energy  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
95 719 2deb (A) 41 657 31.00 0 1 1.15219 -0.63
96 718 1xl7 (A) 13 612 30.00 0 1 1.12259 -0.26
99 720 1ndb (A) 31 625 36.00 0 1 1.18429 -0.24
100 715 1t1u (A) 20 616 33.00 0 1 1.1845 -0.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129452)

Species Accession Gene Product
Candida albicans CaO19.4591   similar to peroxisomal and mitochondrial carnitine O-acetyltransferase
Candida albicans CaO19.12060   similar to peroxisomal and mitochondrial carnitine O-acetyltransferase
Caenorhabditis elegans CELE_B0395.3   Protein B0395.3
Drosophila melanogaster Dmel_CG5122   CG5122 gene product from transcript CG5122-RA
Drosophila melanogaster Dmel_CG1041   CG1041 gene product from transcript CG1041-RA
Drosophila melanogaster Dmel_CG5265   CG5265 gene product from transcript CG5265-RA
Homo sapiens ENSG00000095321   carnitine O-acetyltransferase
Mus musculus ensembl-mmu:ENSMUSG00000026853   carnitine acetyltransferase
Onchocerca volvulus OVOC3406  
Onchocerca volvulus OVOC3409  
Saccharomyces cerevisiae YML042W   carnitine O-acetyltransferase CAT2
Schmidtea mediterranea mk4.025144.00   Choline O-acetyltransferase
Schmidtea mediterranea mk4.014511.00   Choline O-acetyltransferase
Schmidtea mediterranea mk4.005732.00  
Schmidtea mediterranea mk4.004133.03  
Trypanosoma brucei gambiense Tbg972.11.2450   carnitine O-acetyltransferase, putative
Trypanosoma brucei Tb927.11.2230   carnitine O-acetyltransferase, putative
Trypanosoma cruzi TcCLB.511303.40   carnitine O-acetyltransferase, putative
Trypanosoma cruzi TcCLB.508355.40   choline/carnitine O-acetyltransferase, putative
Trypanosoma cruzi TcCLB.511763.19   hypothetical protein, conserved

Essentiality

Tb927.11.2230 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.18.0006 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.18.0006 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.18.0006 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.18.0006 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Choline acetylase 640 aa 31.6% 630 aa Compounds References
Rattus norvegicus Carnitine palmitoyltransferase 2 658 aa 31.6% 621 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Carnitine Acetyltransferase (2.3.1.7 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

7 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.11.2230 (Trypanosoma brucei), carnitine O-acetyltransferase, putative
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