Detailed view for Tb927.10.15270

Basic information

TDR Targets ID: 11153
Trypanosoma brucei, metallo- peptidase, Clan MG, Family M24
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6018 | Length (AA): 395 | MW (Da): 43785 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00557   Metallopeptidase family M24
PF15801   zf-MYND-like zinc finger, mRNA-binding

Gene Ontology

Mouse over links to read term descriptions.
GO:0008235   metalloexopeptidase activity  
GO:0004177   aminopeptidase activity  
GO:0009987   cellular process  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

PDB tag
  • 4FUK:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126721)

Species Accession Gene Product
Arabidopsis thaliana AT2G45240   methionine aminopeptidase 1A
Arabidopsis thaliana AT1G13270   methionine aminopeptidase 1B
Arabidopsis thaliana AT4G37040   methionine aminopeptidase 1D
Babesia bovis BBOV_IV006760   methionine aminopeptidase, putative
Babesia bovis BBOV_III008650   methionine aminopeptidase, putative
Brugia malayi Bm1_31345   Methionine aminopeptidase protein type I
Candida albicans CaO19.10636   methionine aminopeptidase
Candida albicans CaO19.3124   methionine aminopeptidase
Caenorhabditis elegans CELE_Y37E11AL.7   Protein MAP-1
Cryptosporidium hominis Chro.10305   methionine aminopeptidase 1 (MetAP 1) (MAP 1) (Peptidase M 1)
Cryptosporidium parvum cgd1_2700   methionine aminopeptidase with MYND finger at N-terminus
Chlamydia trachomatis CT_851   methionine aminopeptidase
Dictyostelium discoideum DDB_G0279433   methionine aminopeptidase 1
Dictyostelium discoideum DDB_G0280127   methionine aminopeptidase
Drosophila melanogaster Dmel_CG13630   CG13630 gene product from transcript CG13630-RA
Drosophila melanogaster Dmel_CG5188   CG5188 gene product from transcript CG5188-RA
Escherichia coli b0168   methionine aminopeptidase
Echinococcus granulosus EgrG_000923200   methionyl aminopeptidase 1 M24 family
Echinococcus multilocularis EmuJ_000923200   methionyl aminopeptidase 1 (M24 family)
Echinococcus multilocularis EmuJ_000025800   methionyl aminopeptidase 1 (M24 family)
Homo sapiens 254042   methionyl aminopeptidase type 1D (mitochondrial)
Homo sapiens ENSG00000164024   methionyl aminopeptidase 1
Leishmania braziliensis LbrM.19.0860   methionine aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Leishmania donovani LdBPK_190540.1   metallo- peptidase, Clan MG, Family M24
Leishmania infantum LinJ.19.0540   methionine aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Leishmania major LmjF.19.0550   methionine aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Leishmania mexicana LmxM.19.0550   methionine aminopeptidase
Loa Loa (eye worm) LOAG_04834   methionine aminopeptidase type I
Mycobacterium leprae ML1576c   PROBABLE METHIONINE AMINOPEPTIDASE MAPB (MAP) (PEPTIDASE M)
Mycobacterium leprae ML1831c   PROBABLE METHIONINE AMINOPEPTIDASE MAPA (MAP) (PEPTIDASE M) (MetAP)
Mus musculus ENSMUSG00000041921   methionyl aminopeptidase type 1D (mitochondrial)
Mus musculus ENSMUSG00000005813   methionyl aminopeptidase 1
Mycobacterium tuberculosis Rv2861c   Methionine aminopeptidase MapB (map) (peptidase M)
Mycobacterium tuberculosis Rv0734   Methionine aminopeptidase MapA (map) (peptidase M) (MetAP)
Mycobacterium ulcerans MUL_2091   methionine aminopeptidase MapB
Mycobacterium ulcerans MUL_0830   methionine aminopeptidase
Neospora caninum NCLIV_028520   methionine aminopeptidase, putative
Neospora caninum NCLIV_064990   methionine aminopeptidase, putative
Neospora caninum NCLIV_011650   methionine aminopeptidase, putative
Oryza sativa 4330811   Os02g0761700
Oryza sativa 4336948   Os04g0610500
Oryza sativa 4349075   Os10g0508400
Plasmodium berghei PBANKA_1242100   methionine aminopeptidase 1a, putative
Plasmodium berghei PBANKA_1213700   methionine aminopeptidase 1b, putative
Plasmodium falciparum PF3D7_1015300   methionine aminopeptidase 1b, putative
Plasmodium falciparum PF3D7_0527300   methionine aminopeptidase 1a, putative
Plasmodium knowlesi PKNH_1005400   methionine aminopeptidase 1a, putative
Plasmodium knowlesi PKNH_0815400   methionine aminopeptidase 1b, putative
Plasmodium vivax PVX_079875   methionine aminopeptidase 1a, putative
Plasmodium vivax PVX_094985   methionine aminopeptidase 1b, putative
Plasmodium yoelii PY01653   methionine aminopeptidase, type I
Plasmodium yoelii PY04617   methionine aminopeptidase, type I, putative
Saccharomyces cerevisiae YLR244C   Map1p
Schistosoma japonicum Sjp_0097930   ko:K01265 methionyl aminopeptidase [EC3.4.11.18], putative
Schistosoma japonicum Sjp_0305740   ko:K01265 methionyl aminopeptidase [EC3.4.11.18], putative
Schistosoma japonicum Sjp_0108750   Methionine aminopeptidase 1, putative
Schistosoma mansoni Smp_142010.1   methionyl aminopeptidase 1 (M24 family)
Schistosoma mansoni Smp_142010.2   methionyl aminopeptidase 1 (M24 family)
Schmidtea mediterranea mk4.044698.02  
Schmidtea mediterranea mk4.034199.00  
Schmidtea mediterranea mk4.003233.01   Methionine aminopeptidase 1
Trypanosoma brucei gambiense Tbg972.10.18570   methionine aminopeptidase, putative,metallo- peptidase, Clan MG, Family M24
Trypanosoma brucei Tb11.v5.0998   methionine aminopeptidase, putative
Trypanosoma brucei Tb927.10.15270   metallo- peptidase, Clan MG, Family M24
Trypanosoma brucei Tb11.1240   methionine aminopeptidase, type I, putative
Trypanosoma congolense TcIL3000_10_13060   metallo- peptidase, Clan MG, Family M24
Trypanosoma cruzi TcCLB.507031.120   metallo- peptidase, Clan MG, Family M24
Trypanosoma cruzi TcCLB.504111.20   metallo- peptidase, Clan MG, Family M24
Toxoplasma gondii TGME49_248850   methionine aminopeptidase
Toxoplasma gondii TGME49_211330   methionine aminopeptidase
Toxoplasma gondii TGME49_257730   methionine aminopeptidase, type i, putative
Treponema pallidum TP0842   methionine aminopeptidase (map)
Theileria parva TP04_0770   methionine aminopeptidase, type I, putative
Theileria parva TP04_0063   methionine aminopeptidase, type I, putative
Wolbachia endosymbiont of Brugia malayi Wbm0112   methionine aminopeptidase

Essentiality

Tb927.10.15270 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2908 Mycobacterium tuberculosis non-essential nmpdr
Tb927.10.15270 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.15270 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.15270 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.10.15270 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0168 Escherichia coli essential goodall
PBANKA_1213700 Plasmodium berghei Dispensable plasmo
PBANKA_1242100 Plasmodium berghei Essential plasmo
TGME49_211330 Toxoplasma gondii Probably essential sidik
TGME49_248850 Toxoplasma gondii Probably essential sidik
TGME49_257730 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens methionyl aminopeptidase 1 Compounds References
Escherichia coli methionine aminopeptidase Compounds References
Staphylococcus aureus (strain COL) Methionine aminopeptidase Compounds References
Saccharomyces cerevisiae Map1p Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0101 1 1
0.1669 1 1
0.1004 0.6275 0.6275
0.0791 1 1
0.106624 0.921903 0.921886
0.0183 0.4858 1
0.0051 1 1
0.0426 1 1
0.0837 1 0.5
0.1652 0.9357 0.5
0.0243 0.2778 0.5
0.0106 1 1
0.0121 0.3391 0
0.0067 0.302 0
0.0061 0.3151 0
0.0054 1 1
0.1241 1 1
0.112 1 1
0.4505 1 1
0.1276 0.8395 1
0.0061 1 1
0.0936 1 1
0.0149 0.4577 0.5
0.0256 1 0.5
0.0045 1 1
0.0298 0.5451 1
0.0045 0.6018 0.579
0.0179 0.6191 1
0.0106 1 0.5
0.0041 1 0.5
0.0085 1 0.5
0.1389 1 1
0.0102 1 0.5
0.1225 1 1
0.0283 0.9809 0.5
0.0155 1 1
0.0626 0.3715 1
0.1074 1 1
0.0098 1 0.5
0.1361 0.7924 1
0.0049 0.3753 1
0.0123 0.3252 0
0.106624 0.921903 0.921886
0.1318 0.8949 1
0.0187 0.2556 0.5
0.0305 0.3061 1
0.1321 1 1
0.0106 1 0.5
0.0775 1 0.5
0.0054 0.4215 1
0.0465 0.6709 0.5
0.1571 1 1
0.041 0.4708 1
0.1286 1 1
0.0139 1 1
0.0054 1 0.5
0.1318 0.8949 1
0.1149 1 1
0.0106 0.3054 1
0.0331 0.3796 1
0.106624 0.921903 0.921886
0.1646 0.9234 0.5
0.0054 0.9415 1
0.1279 1 0.5
0.007 1 0.5
0.0106 1 0.5
0.018 0.3332 0.3121
0.0054 1 0.5
0.0578 1 1
0.1298 1 0.5
0.0272 0.2994 0.883
0.1077 1 0.5
0.1533 1 0.5
0.022 0.9 1
0.0936 1 1
0.0059 1 1
0.0127 0.5354 1
0.0181 1 0.5
0.0106 1 0.5
0.1304 0.5525 0.5
0.0323 0.646 0.8823
0.0052 0.3649 0.5
0.1589 1 1
0.011 1 1
0.018 0.3332 0.3121
0.0054 0.4297 0.5
0.0674 1 0.5
0.0801 0.9893 1
0.0191 1 1
0.0106 1 0.5
0.0607 1 1
0.0684 1 0.5
0.0084 0.2654 1
0.0952 1 1
0.0354 1 0.5
0.0064 0.3287 0
0.1189 1 1
0.0264 0.9677 0.5
0.0282 0.3944 1
0.1669 1 1
0.0044 0.3031 0.5
0.0055 1 1
0.0145 1 1
0.0046 1 1
0.1063 1 0.5
0.0093 0.5562 1
0.1195 0.3588 1
0.0064 0.3405 0
0.1108 0.5615 0.5
0.1276 0.8395 1
0.0046 0.2734 0.4294
0.0323 1 1
0.0106 1 0.5
0.0106 0.3312 0.5
0.0106 0.5853 1
0.1308 1 0.5
0.0045 1 1
0.112 1 1
0.0936 1 1
0.1225 1 1
0.0466 0.6658 0.5
0.0095 1 1
0.0131 1 1
0.0106 1 0.5
0.0203 0.2783 1
0.0106 1 0.5
0.1147 1 1
0.0186 0.3508 0.5
0.0106 1 0.5
0.0134 0.259 0.5
0.1166 1 0.5
0.0228 0.2626 0.9856
0.0106 1 0.5
0.0106 1 1
0.1276 0.8395 1
0.1292 0.5013 1
0.1195 1 0.5
0.0192 0.5697 1
0.0952 1 1
0.1243 1 1
0.0348 0.4215 1
0.022 0.9 1
0.0137 0.3826 1
0.0232 0.2505 1
0.0086 0.2575 0.4815
0.0179 0.6191 1
0.1589 1 1
0.1258 0.391 1
0.0047 0.3324 0.5
0.0106 1 0.5
0.1389 1 1
0.0046 0.3031 0.5
0.1375 1 1
0.0106 1 0.5
0.0136 0.2798 1
0.0792 0.3269 0.5791
0.0047 0.2806 1
0.0051 1 1
0.0298 0.5451 1
0.1693 0.9585 1
0.0795 1 1
0.0091 1 1
0.0145 0.3595 1
0.0106 0.5352 1
0.0106 1 0.5
0.1757 0.6746 1
0.0227 0.2565 1
0.0052 1 1
0.0097 1 0.5
0.0054 0.3901 0.5
0.1057 1 0.5
0.1646 0.5562 0.5
0.1419 1 0.5
0.0134 0.259 0.5
0.0052 1 1
0.0091 0.6734 1
0.1215 1 0.5
0.0738 0.3935 1
0.0051 1 1
0.0106 1 0.5
0.0061 0.6651 1
0.0106 1 0.5
0.0129 0.411 1
0.0131 0.3213 1
0.0184 0.4106 0.5
0.1265 1 0.5
0.0178 0.6693 0.6693
0.0518 0.3665 1
0.1755 0.7143 0.5
0.0054 0.3316 0.366
0.0052 0.3099 1
0.0725 0.2825 0.5
0.032 0.6613 1
0.0278 0.3131 1
0.1918 1 1
0.188 1 1
0.1697 0.9873 0.5
0.1561 1 1
0.0795 1 1
0.1502 0.7363 0.5
0.0106 1 1
0.1477 1 0.5
0.3038 1 0.5
0.0054 1 0.5
0.1589 1 1
0.1589 1 1
0.0192 0.738 1
0.0061 1 1
0.1057 1 0.5
0.0187 0.3855 0.5
0.0047 1 1
0.0792 0.3269 0.5791
0.0054 0.8928 0
0.103 0.6135 1
0.0054 0.2541 1
0.0199 1 0.5
0.0054 0.2598 0.5
0.0862 0.4038 1
0.0187 0.2556 0.5
0.0475 1 1
0.0792 0.3269 0.5791
0.0125 0.4337 0.4337
0.1589 1 1
0.0158 1 0.5
0.0054 1 0.5
0.0061884 1 0.5
0.132 0.5591 1
0.0323 1 1
0.0271 0.2726 1
0.0776 0.3132 0.5
0.0589 1 0.5
0.0241 0.7655 1
0.0271 1 1
0.144354 1 1
0.0202 0.455 0.4421
0.0052 0.6416 0.6416
0.0179 0.484 1
0.0126 0.3305 0
0.0106 0.5304 1
0.011 1 1
0.1004 0.6275 0.6275
0.006 1 1
0.1361 0.7924 1
0.1486 0.5556 0.5
0.01 1 1
0.1446 1 0.5
0.106624 0.921903 0.921886
0.041 0.4708 1
0.1439 0.2982 1
0.0084 0.512 1
0.106624 0.921903 0.921886
0.0106 1 0.5
0.0298 0.5451 1
0.0272 0.2994 0.883
0.1439 0.2982 1
0.0313 1 1
0.1361 0.7924 1
0.0281736 1 1
0.0055 1 1
0.1353 0.4148 1
0.0625 0.8515 1
0.0128 0.3368 1
0.1114 0.53 0.5
0.0088 0.2994 0.5
0.0121 0.3391 0
0.0106 0.4042 1
0.0877 1 1
0.0626 0.3715 1
0.1147 1 1
0.0262 0.3235 1
0.0267 0.2578 1
0.0173 0.3052 0
0.0777 0.4409 1
0.0087 0.3579 1
0.0192 1 1
0.0054 0.3205 0
0.0106 1 0.5
0.0185 0.4304 0.5
0.1518 1 0.5
0.0106 1 0.5
0.0054 1 0.5
0.0106 0.5853 1
0.0044 0.3031 0.5
0.0274 0.3091 0.7015
0.0298 0.5451 1
0.1024 1 1
0.0106 0.3638 1
0.0815 1 1
0.1478 0.8378 1
0.0137 0.3101 1
0.0982 1 0.5
0.121 1 1
0.0283 0.3008 1
0.0797 1 0.5
0.1439 0.2982 1
0.0103 0.5 0.5
0.0106 1 1
0.0794 1 0.5
0.0132 0.8332 1
0.0044 1 1
0.0298 0.5451 1
0.0187 1 0.5
0.1096 1 0.5
0.1011 0.808 1
0.0234 0.5627 0.5
0.0183 0.4858 1
0.0133 0.3326 1
0.135 0.7736 1
0.0179 0.6191 1
0.3036 0.367 1
0.0218203 1 0.5
0.0088 1 0.5
0.0214 0.265 1
0.0106 1 0.5
0.1439 0.2982 1
0.0291 1 1
0.0045 1 1
0.0054 1 1
0.1693 0.8379 1
0.0239 0.5589 0.5
0.1004 0.6275 0.6275
0.0715 0.3266 1
0.112 1 1
0.0054 1 1
0.1589 1 1
0.1004 0.6275 0.6275
0.0054 1 1
0.0291 1 1
0.019 1 0.5
0.132 0.5591 1
0.0726 0.2787 1
0.1296 1 1
0.0058 0.7229 1
0.0267 0.2578 1
0.0266 0.2931 1
0.0059 0.4461 0.4461
0.1589 1 1
0.214965 1 0.5
0.1627 0.5605 0.5
0.0625 0.8515 1
0.1044 1 0.5
0.0792 0.3269 0.5791
0.0345 1 1
0.0106 1 0.5
0.0106 1 0.5
0.0106 0.4161 0.5
0.0244 0.6475 0.6475
0.1518 1 0.5
0.1189 1 1
0.1113 0.4003 0.5
0.1243 1 1
0.1646 0.5562 0.5
0.1166 1 0.5
0.0274 0.3091 0.7015
0.0091 0.3756 1
0.0183 0.4858 1
0.0106 1 0.5
0.1755 0.7143 0.5
0.0054 1 0.5
0.0106 1 0.5
0.0177 1 0.5
0.0152 1 0.5
0.1258 0.391 1
0.0054 1 0.5
0.2684 1 0.5
0.0052 0.5 0.5
0.0168 0.6126 0.3268
0.1618 0.8551 0.5
0.0231 0.5026 0.5
0.1589 1 1
0.0067 0.302 0
0.0988 0.7376 0.7908
0.1373 0.8181 1
0.1509 0.6583 0.5
0.1081 1 0.5
0.0348 0.4215 1
0.0052 0.5 0.5
0.0158 1 0.5
0.0356 0.3672 0.8158
0.0106 1 0.5
0.0054 1 1
0.0301 0.2596 0.5
0.0103 0.5 0.5
0.0052 1 1
0.0106 1 0.5
0.1667 0.5511 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.10.15270 (Trypanosoma brucei), metallo- peptidase, Clan MG, Family M24
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