Detailed view for Tb927.10.1550

Basic information

TDR Targets ID: 11676
Trypanosoma brucei, proteasome regulatory non-ATP-ase subunit 5

Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5525 | Length (AA): 482 | MW (Da): 54896 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01399   PCI domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 149 5cwj (A) 2 148 12.00 0.86 0.17 0.528679 -1.29
24 171 4y6w (A) 1 154 15.00 0.046 0.16 0.479754 -0.69
47 475 4d10 (D) 4 401 17.00 0 1 0.991942 0.53

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127889)

Species Accession Gene Product
Arabidopsis thaliana AT5G64760   26S proteasome regulatory particle non-ATPase subunit 5B
Arabidopsis thaliana AT5G09900   26S proteasome regulatory particle non-ATPase subunit 5A
Babesia bovis BBOV_III011560   PCI domain containing protein
Brugia malayi Bm1_12660   PCI domain containing protein
Candida albicans CaO19.11515   similar to N terminus of S. cerevisiae RPN5 (YDL147W) subunit of the 19S regulatory particle of the 26S proteasome
Candida albicans CaO19.4034   frameshift
Candida albicans CaO19.11517   frameshift
Candida albicans CaO19.4032   similar to N terminus of S. cerevisiae RPN5 (YDL147W) subunit of the 19S regulatory particle of the 26S proteasome
Caenorhabditis elegans CELE_F10G7.8   Protein RPN-5
Cryptosporidium hominis Chro.10177   hypothetical protein
Cryptosporidium parvum cgd1_1530   Rpn5 like 26S proteasomal regulatory subunit 12, PINT domain containing protein
Dictyostelium discoideum DDB_G0281051   26S proteasome non-ATPase regulatory subunit 12
Drosophila melanogaster Dmel_CG1100   Regulatory particle non-ATPase 5
Echinococcus granulosus EgrG_000421800   26S proteasome non ATPase regulatory subunit 12
Entamoeba histolytica EHI_136180   proteasome regulatory subunit, putative
Echinococcus multilocularis EmuJ_000421800   26S proteasome non ATPase regulatory subunit 12
Homo sapiens 5718   proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
Leishmania braziliensis LbrM.21.0840   proteasome regulatory non-ATP-ase subunit 5, putative,19S proteasome regulatory subunit
Leishmania donovani LdBPK_210840.1   proteasome regulatory non-ATP-ase subunit 5, putative
Leishmania infantum LinJ.21.0840   proteasome regulatory non-ATP-ase subunit 5, putative,19S proteasome regulatory subunit
Leishmania major LmjF.21.0760   proteasome regulatory non-ATP-ase subunit 5, putative,19S proteasome regulatory subunit
Leishmania mexicana LmxM.21.0760   proteasome regulatory non-ATP-ase subunit 5, putative,19S proteasome regulatory subunit
Loa Loa (eye worm) LOAG_05097   PCI domain-containing protein
Mus musculus ENSMUSG00000020720   proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
Neospora caninum NCLIV_026180   hypothetical protein
Oryza sativa 4334802   Os03g0851300
Plasmodium berghei PBANKA_0502200   26S proteasome regulatory subunit p55, putative
Plasmodium falciparum PF3D7_1017900   26S proteasome regulatory subunit p55, putative
Plasmodium knowlesi PKNH_0602100   26S proteasome regulatory subunit p55, putative
Plasmodium vivax PVX_001760   26S proteasome regulatory subunit p55, putative
Plasmodium yoelii PY02643   hypothetical protein
Saccharomyces cerevisiae YDL147W   proteasome regulatory particle lid subunit RPN5
Schistosoma japonicum Sjp_0092210   26S proteasome non-ATPase regulatory subunit 12, putative
Schistosoma japonicum Sjp_0078790   ko:K03035 26S proteasome regulatory subunit N5, putative
Schistosoma mansoni Smp_058650   proteasome regulatory subunit-related
Schmidtea mediterranea mk4.002954.00  
Trypanosoma brucei gambiense Tbg972.10.1830   proteasome regulatory non-ATP-ase subunit 5,19S proteasome regulatory subunit
Trypanosoma brucei Tb927.10.1550   proteasome regulatory non-ATP-ase subunit 5
Trypanosoma congolense TcIL3000_10_1350   proteasome regulatory non-ATP-ase subunit 5, putative
Trypanosoma cruzi TcCLB.506977.90   proteasome regulatory non-ATP-ase subunit 5, putative
Toxoplasma gondii TGME49_261210   26s proteasome subunit p55, putative
Theileria parva TP02_0768   26S proteasome subunit p55, putative
Trichomonas vaginalis TVAG_438030   proteasome regulatory subunits, putative

Essentiality

Tb927.10.1550 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.1550 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.1550 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.1550 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.1550 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F10G7.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_F10G7.8 Caenorhabditis elegans sterile wormbase
YDL147W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0502200 Plasmodium berghei Essential plasmo
TGME49_261210 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 12 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.1550 (Trypanosoma brucei), proteasome regulatory non-ATP-ase subunit 5
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