Detailed view for Tb427tmp.211.5015

Basic information

TDR Targets ID: 11948
Trypanosoma brucei, SLACS reverse transcriptase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.2414 | Length (AA): 636 | MW (Da): 70126 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00078   Reverse transcriptase (RNA-dependent DNA polymerase)

Gene Ontology

Mouse over links to read term descriptions.
GO:0003964   RNA-directed DNA polymerase activity  
GO:0003723   RNA binding  
GO:0006278   RNA-dependent DNA replication  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127518)

Species Accession Gene Product
Leishmania braziliensis LbrM.02.0690   SLACS like gene retrotransposon element
Leishmania braziliensis LbrM.16.1740   hypothetical protein
Leishmania braziliensis LbrM.16.0460   hypothetical protein
Leishmania braziliensis LbrM.02.0760   SLACS like gene retrotransposon element
Leishmania braziliensis LbrM.16.0470   hypothetical protein
Leishmania braziliensis LbrM.16.1730   hypothetical protein
Leishmania braziliensis LbrM.02.0700   hypothetical protein
Leishmania braziliensis LbrM.08.0700   SLACS
Leishmania braziliensis LbrM.16.0780   hypothetical protein
Leishmania braziliensis LbrM.16.0790   hypothetical protein
Leishmania braziliensis LbrM.02.0770   repeat gene hypothetical protein
Leishmania braziliensis LbrM.02.0750   SLACS like gene retrotransposon element
Leishmania braziliensis LbrM.02.0550   Retrotransposable element SLACS
Trypanosoma brucei gambiense Tbg972.9.9200  
Trypanosoma brucei gambiense Tbg972.9.9210   SLACS reverse transcriptase, putative
Trypanosoma brucei Tb927.9.14940   SLACS reverse transcriptase, putative
Trypanosoma brucei Tb427tmp.211.5015   SLACS reverse transcriptase, putative
Trypanosoma congolense TcIL3000_0_32420   SLACS retrotransposable element (part), putative
Trypanosoma congolense TcIL3000_0_43850   SLACS retrotransposable element (part), putative
Trypanosoma congolense TcIL3000_0_23620   SLACS retrotransposable element (part), putative
Trypanosoma congolense TcIL3000_0_32430   SLACS retrotransposable element (part), putative

Essentiality

Tb427tmp.211.5015 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.244.2890 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.244.2890 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.244.2890 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.244.2890 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb09.211.5015 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.5015 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.5015 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.5015 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb427tmp.211.5015 (Trypanosoma brucei), SLACS reverse transcriptase, putative
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