Detailed view for Tb927.6.2840

Basic information

TDR Targets ID: 12048
Trypanosoma brucei, Serine/threonine-protein kinase RIO2, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.556 | Length (AA): 478 | MW (Da): 54932 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01163   RIO1 family
PF09202   Rio2, N-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0003824   catalytic activity  
GO:0005524   ATP binding  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

Ribosome biogenesis in eukaryotes (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127684)

Species Accession Gene Product
Arabidopsis thaliana AT3G51270   protein serine/threonine kinase
Babesia bovis BBOV_III009390   RIO1 family protein
Brugia malayi Bm1_19895   RIO1 family protein
Candida albicans CaO19.6369   similar to S. cerevisiae YNL207W
Candida albicans CaO19.13726   similar to S. cerevisiae YNL207W
Caenorhabditis elegans CELE_Y105E8B.3   Protein RIOK-2
Cryptosporidium parvum cgd7_440   RIO-like kinase domain; N-terminal region conserved
Dictyostelium discoideum DDB_G0282099   RIO family protein kinase
Drosophila melanogaster Dmel_CG11859   RIO kinase 2
Echinococcus granulosus EgrG_000888400   serine:threonine protein kinase rio2
Entamoeba histolytica EHI_170330   RIO1 family protein
Echinococcus multilocularis EmuJ_000888400   serine:threonine protein kinase rio2
Giardia lamblia GL50803_5811   Serine/threonine protein kinase
Homo sapiens ENSG00000058729   RIO kinase 2
Leishmania braziliensis LbrM.30.1480   hypothetical protein, conserved
Leishmania donovani LdBPK_301420.1   Rio2, N-terminal/RIO1 family, putative
Leishmania infantum LinJ.30.1420   hypothetical protein, conserved
Leishmania major LmjF.30.1360   hypothetical protein, conserved
Leishmania mexicana LmxM.29.1360   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_03963   atypical/RIO/RIO2 protein kinase
Mus musculus ENSMUSG00000103104   RIO kinase 2 (yeast)
Neospora caninum NCLIV_045890   RIO1 family domain-containing protein, putative
Oryza sativa 4324901   Os01g0888700
Plasmodium berghei PBANKA_0521400   serine/threonine protein kinase RIO2, putative
Plasmodium falciparum PF3D7_0420100   serine/threonine protein kinase RIO2
Plasmodium knowlesi PKNH_0512800   serine/threonine protein kinase RIO2, putative
Plasmodium vivax PVX_090095   serine/threonine protein kinase RIO2, putative
Plasmodium yoelii PY06287   hypothetical protein
Saccharomyces cerevisiae YNL207W   Rio2p
Schistosoma japonicum Sjp_0013910   ko:K07179 RIO kinase 2, putative
Schistosoma mansoni Smp_043200   serine/threonine-protein kinase rio2 (rio kinase 2)
Schmidtea mediterranea mk4.000765.01   Serine/threonine-protein kinase RIO2
Trypanosoma brucei gambiense Tbg972.6.2610   hypothetical protein, conserved
Trypanosoma brucei Tb927.6.2840   Serine/threonine-protein kinase RIO2, putative
Trypanosoma congolense TcIL3000_6_2320   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511753.70   serine/threonine protein kinase RIO2, putative
Trypanosoma cruzi TcCLB.507021.10   serine/threonine protein kinase RIO2, putative
Toxoplasma gondii TGME49_227260   RIO1 family protein
Theileria parva TP04_0848   hypothetical protein, conserved
Trichomonas vaginalis TVAG_332390   RIO family atypical protein kinase

Essentiality

Tb927.6.2840 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.2840 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.6.2840 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.2840 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.6.2840 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y105E8B.3 Caenorhabditis elegans embryonic lethal wormbase
YNL207W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0521400 Plasmodium berghei Essential plasmo
TGME49_227260 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens RIO kinase 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.6.2840 (Trypanosoma brucei), Serine/threonine-protein kinase RIO2, putative
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