Detailed view for Tb927.11.15530

Basic information

TDR Targets ID: 12943
Trypanosoma brucei, C-14 sterol reductase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.4645 | Length (AA): 458 | MW (Da): 51183 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 6

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01222   Ergosterol biosynthesis ERG4/ERG24 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0050613   delta14-sterol reductase activity  
GO:0016628   oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor  
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0006696   ergosterol biosynthetic process  
GO:0055114   oxidation reduction  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
51 458 4quv (A) 24 427 37.00 0 1 1.26223 0.38

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128488)

Species Accession Gene Product
Arabidopsis thaliana AT3G52940   delta(14)-sterol reductase
Candida albicans CaO19.1598   similar to S. cerevisiae sterol C-14 reductase ERG24 (YNL280C) involved in ergosterol biosynthesis
Candida albicans CaO19.9166   similar to S. cerevisiae sterol C-14 reductase ERG24 (YNL280C) involved in ergosterol biosynthesis
Dictyostelium discoideum DDB_G0284407   Delta-sterol reductase
Dictyostelium discoideum DDB_G0267448   erg4/erg24 family protein
Drosophila melanogaster Dmel_CG17952   Lamin B receptor
Echinococcus granulosus EgrG_000379500   lamin b receptor
Echinococcus multilocularis EmuJ_000379500   lamin b receptor
Homo sapiens ENSG00000149809   transmembrane 7 superfamily member 2
Homo sapiens ENSG00000143815   lamin B receptor
Leishmania braziliensis LbrM.32.2550   C-14 sterol reductase, putative
Leishmania donovani LdBPK_322470.1   C-14 sterol reductase, putative
Leishmania infantum LinJ.32.2470   C-14 sterol reductase, putative
Leishmania major LmjF.32.2320   C-14 sterol reductase, putative
Leishmania mexicana LmxM.31.2320   C-14 sterol reductase, putative
Mus musculus ENSMUSG00000024799   transmembrane 7 superfamily member 2
Mus musculus ENSMUSG00000004880   lamin B receptor
Oryza sativa 9270556   Os01g0354200
Saccharomyces cerevisiae YNL280C   delta(14)-sterol reductase
Schistosoma japonicum Sjp_0205080   ko:K00222 delta14-sterol reductase [EC1.3.1.70], putative
Schistosoma japonicum Sjp_0317000   Lamin-B receptor, putative
Schistosoma mansoni Smp_124300   Lamin B receptor (ERG24)
Schmidtea mediterranea mk4.000442.04   Lamin-B receptor
Trypanosoma brucei gambiense Tbg972.11.17430   C-14 sterol reductase, putative
Trypanosoma brucei Tb927.11.15530   C-14 sterol reductase, putative
Trypanosoma congolense TcIL3000.11.15590   C-14 sterol reductase, putative
Trypanosoma congolense TcIL3000_0_39020   Ergosterol biosynthesis ERG4/ERG24 family, putative
Trypanosoma cruzi TcCLB.507969.60   C-14 sterol reductase, putative
Trypanosoma cruzi TcCLB.507129.30   C-14 sterol reductase, putative

Essentiality

Tb927.11.15530 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.7170 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.7170 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.7170 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.7170 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YNL280C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Zea mays Delta(14)-sterol reductase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0241 0.2833 0.4347
0.011 0.5 0.5
0.0097 0.574 0.5145
0.0241 0.2833 0.4347

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.15530 (Trypanosoma brucei), C-14 sterol reductase, putative
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