Detailed view for Tb11.v5.0206

Basic information

TDR Targets ID: 13172
Trypanosoma brucei, phosphatidylinositolN-acetylglucosaminyltransferase, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 7.2839 | Length (AA): 342 | MW (Da): 38507 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 6

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF06432   Phosphatidylinositol N-acetylglucosaminyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0017176   phosphatidylinositol N-acetylglucosaminyltransferase activity  
GO:0006506   GPI anchor biosynthetic process  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
15 79 4gzr (B) 6 89 22.00 0 0.06 0.407358 -0.69
61 313 5ckr (A) 43 308 11.00 0.68 0.05 0.762066 -0.18
62 199 2dhh (A) 358 489 33.00 0.13 0.04 0.365409 1.52

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128704)

Species Accession Gene Product
Arabidopsis thaliana AT2G34980   phosphatidylinositol glycan, class C
Babesia bovis BBOV_IV011600   hypothetical protein
Brugia malayi Bm1_32160   hypothetical protein
Candida albicans CaO19.538   synthesis of GlcNAc phosphotidylinositol
Candida albicans CaO19.8171   synthesis of GlcNAc phosphotidylinositol
Caenorhabditis elegans CELE_T20D3.8   Protein T20D3.8
Cryptosporidium hominis Chro.10380   phosphatidylinositol-glycan-class c
Cryptosporidium parvum cgd1_3380   phosphatidylinositol-glycan-class c, pigC, 8x transmembrane domains
Dictyostelium discoideum DDB_G0286221   GlcNAc transferase
Drosophila melanogaster Dmel_CG12077   Phosphatidylinositol glycan anchor biosynthesis, class C ortholog (H. sapiens)
Echinococcus granulosus EgrG_001029700   phosphatidylinositol
Entamoeba histolytica EHI_031520   phosphatidylinositol N-acetylglucosaminyltransferase subunit C, putative
Echinococcus multilocularis EmuJ_001029700   phosphatidylinositol
Homo sapiens ENSG00000135845   phosphatidylinositol glycan anchor biosynthesis, class C
Leishmania braziliensis LbrM.35.1900   phosphatidylinositolN-acetylglucosaminyltransfe ra sesubunit c, putative
Leishmania donovani LdBPK_361790.1   phosphatidylinositolN-acetylglucosaminyltransfe ra sesubunit c, putative
Leishmania infantum LinJ.36.1790   phosphatidylinositolN-acetylglucosaminyltransfe ra sesubunit c, putative
Leishmania major LmjF.36.1710   phosphatidylinositolN-acetylglucosaminyltransfe ra sesubunit c, putative
Leishmania mexicana LmxM.36.1710   phosphatidylinositolN-acetylglucosaminyltransfe ra sesubunit c, putative
Loa Loa (eye worm) LOAG_02980   hypothetical protein
Mus musculus ENSMUSG00000026698   phosphatidylinositol glycan anchor biosynthesis, class C
Neospora caninum NCLIV_009460   phosphatidylinositol N- acetylglucosaminyltransferase subunit, putative
Oryza sativa 4330019   Os02g0622200
Plasmodium berghei PBANKA_0812100   phosphatidylinositol N-acetylglucosaminyltransferase, putative
Plasmodium falciparum PF3D7_0911000   phosphatidylinositol N-acetylglucosaminyltransferase, putative
Plasmodium knowlesi PKNH_0709000   phosphatidylinositol N-acetylglucosaminyltransferase, putative
Plasmodium vivax PVX_098990   phosphatidylinositol N-acetylglucosaminyltransferase, putative
Saccharomyces cerevisiae YPL076W   Gpi2p
Schistosoma japonicum Sjp_0216060   ko:K03859 phosphatidylinositol glycan, class C, putative
Schmidtea mediterranea mk4.001621.07  
Trypanosoma brucei gambiense Tbg972.10.7510   phosphatidylinositolN- acetylglucosaminyltransferase subunit c, putative
Trypanosoma brucei Tb11.v5.0206   phosphatidylinositolN-acetylglucosaminyltransferase, putative
Trypanosoma congolense TcIL3000_10_5190   phosphatidylinositolN- acetylglucosaminyltransferase subunit c, putative
Trypanosoma cruzi TcCLB.503781.20   phosphatidylinositol N-acetylglucosaminyltransferase subunit C, putative
Trypanosoma cruzi TcCLB.507639.100   phosphatidylinositol N-acetylglucosaminyltransferase subunit C, putative
Toxoplasma gondii TGME49_299040   phosphatidylinositol n-acetylglucosaminyltransferase
Theileria parva TP01_1147   hypothetical protein

Essentiality

Tb11.v5.0206 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.6140 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.6140 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.6140 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.6140 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YPL076W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0812100 Plasmodium berghei Essential plasmo
TGME49_299040 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb11.v5.0206 (Trypanosoma brucei), phosphatidylinositolN-acetylglucosaminyltransferase, putative
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