Detailed view for Tb927.11.250

Basic information

TDR Targets ID: 13177
Trypanosoma brucei, ubiquitin carboxyl-terminal hydrolase, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 4.5287 | Length (AA): 236 | MW (Da): 25978 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Pfam domains

PF01088   Ubiquitin carboxyl-terminal hydrolase, family 1

Gene Ontology

Mouse over links to read term descriptions.
GO:0004843   ubiquitin-specific protease activity  
GO:0005737   cytoplasm  
GO:0005622   intracellular  
GO:0004629   phospholipase C activity  
GO:0004221   ubiquitin thiolesterase activity  
GO:0007242   intracellular signaling cascade  
GO:0006629   lipid metabolic process  
GO:0006511   ubiquitin-dependent protein catabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127717)

Species Accession Gene Product
Arabidopsis thaliana AT4G17510   ubiquitin C-terminal hydrolase 3
Babesia bovis BBOV_III010630   ubiquitin carboxyl-terminal hydrolase, family 1 protein
Brugia malayi Bm1_09170   Ubiquitin carboxyl-terminal hydrolase, family 1 protein
Candida albicans CaO19.13651   potential ubiquitin carboxyl-terminal hydrolase similar to S. cerevisiae YUH1 (YJR099W)
Candida albicans CaO19.6272   potential ubiquitin carboxyl-terminal hydrolase similar to S. cerevisiae YUH1 (YJR099W)
Caenorhabditis elegans CELE_Y40G12A.1   Protein UBH-3
Caenorhabditis elegans CELE_F46E10.8   Protein UBH-1
Cryptosporidium hominis Chro.80372   ubiquitin carboxy-terminal hydrolase L1; gracile axonal dystrophy; protein gene product 9.5
Cryptosporidium parvum cgd8_3200   ubiquitin C-terminal hydrolase of the cysteine proteinase fold
Dictyostelium discoideum DDB_G0282007   peptidase C12 family protein
Drosophila melanogaster Dmel_CG4265   Ubiquitin carboxy-terminal hydrolase
Echinococcus granulosus EgrG_001029600   ubiquitin carboxyl terminal hydrolase isozyme
Echinococcus multilocularis EmuJ_001029600   ubiquitin carboxyl terminal hydrolase isozyme
Homo sapiens ENSG00000118939   ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Homo sapiens ENSG00000154277   ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Leishmania braziliensis LbrM.25.0190   ubiquitin carboxyl-terminal hydrolase, putative,cysteine peptidase, Clan CA, family C12, putative
Leishmania donovani LdBPK_250190.1   ubiquitin carboxyl-terminal hydrolase, putative
Leishmania infantum LinJ.25.0190   ubiquitin carboxyl-terminal hydrolase, putative,cysteine peptidase, Clan CA, family C12, putative
Leishmania major LmjF.25.0190   ubiquitin carboxyl-terminal hydrolase, putative,cysteine peptidase, Clan CA, family C12, putative
Leishmania mexicana LmxM.25.0190   ubiquitin carboxyl-terminal hydrolase, putative,cysteine peptidase, Clan CA, family C12, putative
Loa Loa (eye worm) LOAG_03494   hypothetical protein
Mus musculus ENSMUSG00000029223   ubiquitin carboxy-terminal hydrolase L1
Mus musculus ENSMUSG00000022111   ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Neospora caninum NCLIV_024470   hypothetical protein
Oryza sativa 4337328   Os04g0667500
Oryza sativa 4330187   Os02g0654500
Plasmodium berghei PBANKA_1324100   ubiquitin carboxyl-terminal hydrolase isozyme L3, putative
Plasmodium falciparum PF3D7_1460400   ubiquitin carboxyl-terminal hydrolase isozyme L3
Plasmodium knowlesi PKNH_1221300   ubiquitin carboxyl-terminal hydrolase isozyme L3, putative
Plasmodium vivax PVX_117435   ubiquitin carboxyl-terminal hydrolase isozyme L3, putative
Plasmodium yoelii PY01755   hypothetical protein
Saccharomyces cerevisiae YJR099W   Yuh1p
Schistosoma japonicum Sjp_0205820   ko:K05609 ubiquitin carboxyl-terminal hydrolase L3, putative
Schistosoma mansoni Smp_168800.4   family C12 unassigned peptidase (C12 family)
Schistosoma mansoni Smp_168800.2   family C12 unassigned peptidase (C12 family)
Schistosoma mansoni Smp_168800.1   family C12 unassigned peptidase (C12 family)
Schmidtea mediterranea mk4.000885.12   Ubiquitin carboxyl-terminal hydrolase
Trypanosoma brucei gambiense Tbg972.11.120   ubiquitin carboxyl-terminal hydrolase, putative
Trypanosoma brucei Tb927.11.250 this record   ubiquitin carboxyl-terminal hydrolase, putative
Trypanosoma congolense TcIL3000.11.150   ubiquitin carboxyl-terminal hydrolase, putative
Trypanosoma cruzi TcCLB.504109.90   ubiquitin carboxyl-terminal hydrolase, putative
Toxoplasma gondii TGME49_263470   ubiquitin carboxyl-terminal hydrolase UCHL3
Trichomonas vaginalis TVAG_204450   Clan CA, family C12, ubiquitin hydrolase-like cysteine peptidase
Trichomonas vaginalis TVAG_455660   Clan CA, family C12, ubiquitin hydrolase-like cysteine peptidase

Essentiality

Tb927.11.250 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.03.0890 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.03.0890 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.03.0890 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.03.0890 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_263470 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
 
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Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) Compounds References
Homo sapiens ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) Compounds References
Leishmania major ubiquitin carboxyl-terminal hydrolase, putative,cysteine peptidase, Clan CA, family C12, putative Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.11.250 (Trypanosoma brucei), ubiquitin carboxyl-terminal hydrolase, putative
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