Detailed view for Tb927.7.2250

Basic information

TDR Targets ID: 13511
Trypanosoma brucei, carnitine O-palmitoyltransferase II, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.0376 | Length (AA): 668 | MW (Da): 75171 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00755   Choline/Carnitine o-acyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016746   transferase activity, transferring acyl groups  
GO:0008415   acyltransferase activity  

Metabolic Pathways

Fatty acid metabolism (KEGG)
Fatty acid metabolism (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
37 667 2deb (A) 32 652 36.00 0 1 1.43071 -0.8

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129195)

Species Accession Gene Product
Brugia malayi Bm1_19470   Choline/Carnitine o-acyltransferase family protein
Candida albicans CaO19.12026   similar to mitochondrial outer carnitine acetyltransferase
Candida albicans CaO19.3361   N terminus of outer carnitine acetyltransferase
Candida albicans CaO19.4551   similar to mitochondrial outer carnitine acetyltransferase
Candida albicans CaO19.361   C terminus of mitochondrial carnitine acetyltransferase
Candida albicans CaO19.10869   N terminus of outer carnitine acetyltransferase
Caenorhabditis elegans CELE_R07H5.2   Protein CPT-2, isoform B
Drosophila melanogaster Dmel_CG2107   CG2107 gene product from transcript CG2107-RA
Echinococcus granulosus EgrG_001061200   carnitine O palmitoyltransferase 2
Echinococcus multilocularis EmuJ_001061200   carnitine O palmitoyltransferase 2
Homo sapiens ENSG00000157184   carnitine palmitoyltransferase 2
Leishmania braziliensis LbrM.22.0300   carnitine palmitoyltransferase-like protein
Leishmania donovani LdBPK_220190.1   carnitine palmitoyltransferase-like protein
Leishmania infantum LinJ.22.0190   carnitine palmitoyltransferase-like protein
Leishmania major LmjF.22.0310   carnitine palmitoyltransferase-like protein
Leishmania mexicana LmxM.22.0310   carnitine palmitoyltransferase-like protein
Loa Loa (eye worm) LOAG_06314   choline/Carnitine O-acyltransferase
Mus musculus ENSMUSG00000028607   carnitine palmitoyltransferase 2
Saccharomyces cerevisiae YAR035W   carnitine O-acetyltransferase YAT1
Schmidtea mediterranea mk4.001868.10   Carnitine O-palmitoyltransferase 2, mitochondrial
Schmidtea mediterranea mk4.001868.06  
Schmidtea mediterranea mk4.001868.07   Carnitine O-palmitoyltransferase 2, mitochondrial
Schmidtea mediterranea mk4.001868.14  
Schmidtea mediterranea mk4.001868.12  
Trypanosoma brucei gambiense Tbg972.7.2510   carnitine O-palmitoyltransferase II, putative
Trypanosoma brucei Tb927.7.2250   carnitine O-palmitoyltransferase II, putative
Trypanosoma congolense TcIL3000_7_1560   carnitine O-palmitoyltransferase II, putative
Trypanosoma cruzi TcCLB.511807.284   carnitine O-palmitoyltransferase II, putative

Essentiality

Tb927.7.2250 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.2250 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.2250 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.2250 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.2250 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_R07H5.2 Caenorhabditis elegans embryonic lethal wormbase
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens carnitine palmitoyltransferase 2 Compounds References
Rattus norvegicus Carnitine palmitoyltransferase 2 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Choline acetylase 640 aa 29.3% 641 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0135 0.3893 1
0.0262 0.3516 1
0.2067 0.3226 1
0.2067 0.2806 1
0.0748 0.4025 1
0.0483 0.3267 1
0.0223 0.3086 1
0.0271 0.5118 0.5
0.311 0.5718 1
0.0268 0.6398 1
0.2415 0.2687 1
0.264 0.5527 1
0.0279 0.5203 1
0.3003 0.3189 1
0.0307 0.3155 1
0.021 0.351 1
0.0155 0.4015 1
0.0304 0.2864 1
0.032 0.3638 1
0.2071 0.2814 1
0.2024 0.3129 1
0.0299 0.3284 1
0.1508 0.2608 1
0.0163 0.2637 1
0.0482 0.5572 1
0.0472 0.2752 0.8192
0.0208 0.2757 1
0.0248 0.3574 1
0.03 0.2687 1
0.1265 0.2713 1
0.1508 0.2608 1
0.0943 0.2983 1
0.0279 0.5445 1
0.1609 0.2574 1
0.0309 0.3529 1
0.0304 0.2864 1
0.2067 0.2806 1
0.1932 0.2551 1
0.0114 0.3882 1
0.1881 0.2952 1
0.0342 0.3047 0.6395
0.2768 0.3129 1
0.0309 0.3538 1
0.1098 0.3571 1
0.0609 0.368 1
0.1508 0.2608 1
0.2067 0.2806 1
0.0279 0.4351 1
0.032 0.2639 1
0.3545 0.3221 1
0.0456 0.2936 1
0.1508 0.2608 1
0.0304 0.303 1
0.281 0.316 1
0.0663 0.2632 1
0.9685 0.3112 1
0.0522 0.5621 1
0.1508 0.2608 1
0.3606 0.2978 1
0.0111 0.3086 1
0.2972 0.3164 1
0.2067 0.2806 1
0.0253 0.2959 0.7454
0.4242 0.5724 1
0.0222 0.716 1
0.0221 0.6836 1
0.3545 0.3221 1
0.1098 0.3571 1
0.1508 0.2608 1
0.3133 0.3166 1
0.4013 0.2921 1
0.0342 0.2893 1
0.1265 0.2713 1
0.0201 0.3086 1
0.3373 0.2684 1
0.0373 0.279 1
0.0724 0.5722 1
0.0255 0.5267 1
0.1856 0.3521 1
0.3606 0.2978 1
0.0163 0.323 1
0.032 0.2639 1
0.2384 0.3412 1
0.0553 0.5498 1
0.0294 0.2824 1
0.031 0.2646 1
0.0099 0.2774 1
0.0223 0.7233 1
0.0223 0.6584 1
0.0291 0.2779 0.5
0.3545 0.3221 1
0.034 0.2739 1
0.2067 0.3226 1
0.1027 0.3457 1
0.0996 0.2782 0.6175
0.2072 0.317 1
0.2072 0.317 1
0.2266 0.3209 1
0.0175 0.2967 1
0.2108 0.3003 1
0.0996 0.2782 0.6175
0.0143 0.2767 0.3137
0.0201 0.5 0.5
0.032 0.2687 1
0.2221 0.313 1
0.0262 0.3516 1
0.2067 0.2806 1
0.014 0.2771 1
0.0482 0.5572 1
0.0253 0.3104 1
0.2141 0.3655 1
0.0223 0.7233 1
0.0289 0.7602 1
0.3906 0.3089 1
0.0146 0.271 0.7944
0.0271 0.5118 0.5
0.0671 0.4016 1
0.4139 0.5648 1
0.8562 0.2797 1
0.402 0.2962 1
0.2067 0.2806 1
0.0489 0.4305 0.5
0.0271 0.5118 0.5
0.0291 0.5276 1
0.9124 0.2966 1
0.208 0.2807 1
0.1508 0.2608 1
0.031 0.2981 1
0.0943 0.2983 1
0.035 0.3124 1
0.021 0.351 1
0.2384 0.3412 1
0.3545 0.3221 1
0.0289 0.2542 1
0.032 0.3043 1
0.1508 0.2608 1
0.1932 0.2551 1
0.2067 0.2806 1
0.2221 0.313 1
0.0462 0.2767 0.4491
0.0525 0.4157 1
0.1122 0.3226 1
0.0244 0.429 1
0.0662 0.2614 1
0.0271 0.5118 0.5
0.1508 0.2608 1
0.1508 0.2608 1
0.0262 0.3516 1
0.0227 0.5279 1
0.0518 0.2698 1
0.3545 0.3221 1
0.1508 0.2608 1
0.0229 0.5397 1
0.0289 0.7602 1
0.0864 0.3059 1
0.311 0.5718 1
0.402 0.2962 1
0.1192 0.55 1
0.0268 0.2687 1
0.0518 0.2698 1
0.0304 0.2864 1
0.0244 0.2949 1
0.0373 0.279 1
0.1074 0.5281 1
0.1405 0.2553 1
0.208 0.2807 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.7.2250 (Trypanosoma brucei), carnitine O-palmitoyltransferase II, putative
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