Detailed view for PF3D7_1020800

Basic information

TDR Targets ID: 1481
Plasmodium falciparum, dihydrolipoamide acyltransferase component E2
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.7733 | Length (AA): 640 | MW (Da): 73890 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00198   2-oxoacid dehydrogenases acyltransferase (catalytic domain)
PF00364   Biotin-requiring enzyme

Gene Ontology

Mouse over links to read term descriptions.
GO:0020011   apicoplast  
GO:0016746   transferase activity, transferring acyl groups  
GO:0005739   mitochondrion  
GO:0008415   acyltransferase activity  
GO:0005515   protein binding  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
53 141 1y8o (B) 133 227 30.00 0.000013 0.94 0.57 -0.58
181 262 1qjo (A) 1 80 24.00 0.000000000017 0.99 0.53 -0.92
181 265 1k8m (A) 3 87 25.00 0.0000003 1 0.48 -0.21
354 395 1w85 (I) 128 169 26.00 0.0000028 0.77 0.62 -2.23
406 640 1eaf () 396 637 28.00 0 1 0.76 -0.79
417 639 1scz (A) 173 402 22.00 0 1 0.57 -0.97
51 133 2dnc (A) 6 88 42.00 0.0000058 1 0.643588 -0.2
136 634 3s5h (A) 351 962 27.00 0.21 1 0.703587 1.3
198 248 2d5d (A) 87 137 39.00 0.65 0.82 0.523588 0.22
427 639 3l60 (A) 174 391 20.00 0 1 0.657912 -0.63

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile gametocyte, sporozoite, Oocyst, Sporozoite. PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile late ring, Female Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile early schizont, early trophozoite, late schizont, late trophozoite, Ring. PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 40 hs, Male Gametocyte. Otto TD Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs. Otto TD
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_134960)

Species Accession Gene Product
Arabidopsis thaliana AT3G25860   dihydrolipoamide S-acetyltransferase
Arabidopsis thaliana AT1G34430   protein embryo defective 3003
Neospora caninum NCLIV_044290   Pyruvate dehydrogenase E2 component, related
Oryza sativa 4345640   Os08g0431300
Oryza sativa 4347022   Os09g0408600
Oryza sativa 4351678   Os12g0182200
Plasmodium berghei PBANKA_0505000   dihydrolipoamide acyltransferase, putative
Plasmodium falciparum PF3D7_1020800   dihydrolipoamide acyltransferase component E2
Plasmodium knowlesi PKNH_0605000   dihydrolipoamide acetyltransferase, putative
Plasmodium yoelii PY04573   putative dihydrolipoamide S-acetyltransferase
Toxoplasma gondii TGME49_206610   pyruvate dehydrogenase complex subunit PDH-E2

Essentiality

PF3D7_1020800 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0505000 Plasmodium berghei Dispensable plasmo
TGME49_206610 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_1020800 (Plasmodium falciparum), dihydrolipoamide acyltransferase component E2
Title for this comment
Comment