Detailed view for Tb927.7.2550

Basic information

TDR Targets ID: 14957
Trypanosoma brucei, proteasome regulatory ATPase subunit 5
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.604 | Length (AA): 445 | MW (Da): 49150 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)

Gene Ontology

Mouse over links to read term descriptions.
GO:0036402   GO:proteasome-activating ATPase activity  

GO:0005737   cytoplasm  
GO:0005634   nucleus  
GO:0017111   nucleoside-triphosphatase activity  
GO:0016787   hydrolase activity  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  
GO:0030163   protein catabolic process  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 444 5c19 (D) 282 755 25.00 0.00022 1 1.22481 0.71
179 438 3h4m (A) 158 416 54.00 0 1 1.30407 -0.86
180 440 3whk (A) 170 429 54.00 0 1 1.18692 -0.51
361 438 4a3v (B) 378 455 41.00 0 1 0.784681 -1.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127786)

Species Accession Gene Product
Arabidopsis thaliana AT3G05530   regulatory particle triple-A ATPase 5A
Arabidopsis thaliana AT1G09100   26S proteasome AAA-ATPase subunit RPT5B
Babesia bovis BBOV_IV004580   26S protease regulatory subunit 6A, putative
Brugia malayi Bm1_11555   26S protease regulatory subunit 6A
Candida albicans CaO19.10635   likely ATPase similar to S. cerevisiae RPT5 (YOR117W) ATPase subunit of the 19S regulatory particle of the 26S proteasome
Candida albicans CaO19.3123   likely ATPase similar to S. cerevisiae RPT5 (YOR117W) ATPase subunit of the 19S regulatory particle of the 26S proteasome
Caenorhabditis elegans CELE_F56H1.4   Protein RPT-5
Cryptosporidium hominis Chro.20147   26S protease subunit regulatory subunit 6a
Cryptosporidium parvum cgd2_1350   26S proteasome regulatory subunit, S6a like AAA ATpase
Dictyostelium discoideum DDB_G0284415   26S protease regulatory subunit 6A
Drosophila melanogaster Dmel_CG10370   Regulatory particle triple-A ATPase 5
Echinococcus granulosus EgrG_001190500   26s protease regulatory subunit 6a
Entamoeba histolytica EHI_053020   26S protease regulatory subunit, putative
Echinococcus multilocularis EmuJ_001190500   26s protease regulatory subunit 6a
Giardia lamblia GL50803_4365   26S protease regulatory subunit 6A
Homo sapiens ENSG00000165916   proteasome (prosome, macropain) 26S subunit, ATPase, 3
Leishmania braziliensis LbrM.22.0560   proteasome regulatory ATPase subunit 5, putative
Leishmania donovani LdBPK_220490.1   proteasome regulatory ATPase subunit 5, putative
Leishmania infantum LinJ.22.0490   proteasome regulatory ATPase subunit 5, putative
Leishmania major LmjF.22.0620   proteasome regulatory ATPase subunit 5, putative
Leishmania mexicana LmxM.22.0620   proteasome regulatory ATPase subunit 5, putative
Loa Loa (eye worm) LOAG_11027   26S protease regulatory subunit 6A
Mus musculus ENSMUSG00000002102   proteasome (prosome, macropain) 26S subunit, ATPase 3
Neospora caninum NCLIV_060380   hypothetical protein
Oryza sativa 4331050   Os02g0803700
Oryza sativa 4340276   Os06g0173100
Plasmodium berghei PBANKA_0917900   26S protease regulatory subunit 6A, putative
Plasmodium falciparum PF3D7_1130400   26S protease regulatory subunit 6A, putative
Plasmodium knowlesi PKNH_0928500   26S protease regulatory subunit 6A, putative
Plasmodium vivax PVX_092125   26S protease regulatory subunit 6a, putative
Plasmodium yoelii PY00657   26s protease regulatory subunit 6a (tat-binding protein homolog 1) (tbp-1). [baker's yeast
Saccharomyces cerevisiae YOR117W   proteasome regulatory particle base subunit RPT5
Schistosoma japonicum Sjp_0003450   ko:K03065 26S proteasome regulatory subunit T5, putative
Schistosoma mansoni Smp_042270.3   26S protease regulatory subunit 6a
Schistosoma mansoni Smp_042270.1   26S protease regulatory subunit 6a
Schistosoma mansoni Smp_042270.2   26S protease regulatory subunit 6a
Schmidtea mediterranea mk4.003371.04   26S protease regulatory subunit 6A
Schmidtea mediterranea mk4.005171.07  
Schmidtea mediterranea mk4.020865.00   26S protease regulatory subunit 6A
Schmidtea mediterranea mk4.039848.00  
Schmidtea mediterranea mk4.019442.02   26S protease regulatory subunit 6A
Schmidtea mediterranea mk4.007989.01  
Schmidtea mediterranea mk4.005188.03   26S protease regulatory subunit 6A
Schmidtea mediterranea mk4.009865.00  
Trypanosoma brucei gambiense Tbg972.7.2860   proteasome regulatory ATPase subunit 5
Trypanosoma brucei Tb927.7.2550   proteasome regulatory ATPase subunit 5
Trypanosoma congolense TcIL3000_0_43330   proteasome regulatory ATPase subunit 5
Trypanosoma congolense TcIL3000_7_1890   ATPase family associated with various cellular activities (AAA), putative
Trypanosoma cruzi TcCLB.511813.30   proteasome regulatory ATPase subunit 5, putative
Trypanosoma cruzi TcCLB.506859.20   proteasome regulatory ATPase subunit 5, putative
Toxoplasma gondii TGME49_300310   26S proteasome regulatory subunit, S6a family AAA ATpase
Theileria parva TP01_0290   26S proteasome subunit, putative
Trichomonas vaginalis TVAG_035330   26S protease regulatory subunit, putative

Essentiality

Tb927.7.2550 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.2550 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.2550 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.2550 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.2550 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F56H1.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_F56H1.4 Caenorhabditis elegans larval lethal wormbase
CELE_F56H1.4 Caenorhabditis elegans slow growth wormbase
CELE_F56H1.4 Caenorhabditis elegans sterile wormbase
YOR117W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0917900 Plasmodium berghei Essential plasmo
TGME49_300310 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 3 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.7.2550 (Trypanosoma brucei), proteasome regulatory ATPase subunit 5
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