Detailed view for Tb927.11.7270

Basic information

TDR Targets ID: 14994
Trypanosoma brucei, proteasome beta 3 subunit, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 4.6971 | Length (AA): 205 | MW (Da): 22484 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00227   Proteasome subunit

Gene Ontology

Mouse over links to read term descriptions.
GO:0019774   proteasome core complex, beta-subunit complex  
GO:0005839   proteasome core complex  
GO:0004298   threonine endopeptidase activity  
GO:0004175   endopeptidase activity  
GO:0051603   proteolysis involved in cellular protein catabolic process  
GO:0043161   proteasomal ubiquitin-dependent protein catabolic process  
GO:0006511   ubiquitin-dependent protein catabolic process  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 205 5le5 (L) 2 213 26.00 0 1 1.3492 -0.25
2 205 5le5 (I) 1 204 45.00 0 1 1.66152 -1.23
3 205 1ryp (J) 0 196 45.00 0 1 1.66044 -1.26
130 190 4hwv (A) 111 193 46.00 0.89 0.39 0.268961 1.21

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

  • 1FNT:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
  • 1Z7Q:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127761)

Species Accession Gene Product
Arabidopsis thaliana AT1G21720   proteasome beta subunit C1
Arabidopsis thaliana AT1G77440   20S proteasome beta subunit C2
Babesia bovis BBOV_III007090   proteasome A-type and B-type family protein
Brugia malayi Bm1_24805   proteasome subunit beta type 3
Candida albicans CaO19.1336   peptidase,20S proteasome subunit, protein degradation
Candida albicans CaO19_1336   hypothetical protein
Candida albicans CaO19.8916   peptidase, 20S proteasome subunit, protein degradation
Caenorhabditis elegans CELE_Y38A8.2   Protein PBS-3
Cryptosporidium hominis Chro.20061   proteasome component
Cryptosporidium parvum cgd2_530   possible proteasome component
Dictyostelium discoideum DDB_G0269772   20S proteasome subunit beta-3
Drosophila melanogaster Dmel_CG11981   Proteasome beta3 subunit
Echinococcus granulosus EgrG_001064900   proteasome subunit beta t family
Entamoeba histolytica EHI_049330   proteasome subunit beta type 3, putative
Echinococcus multilocularis EmuJ_001064900   proteasome subunit beta (t family) proteasome (prosome macropain) subunit beta
Giardia lamblia GL50803_13756   Proteasome subunit beta type 3
Homo sapiens ENSG00000277791   proteasome (prosome, macropain) subunit, beta type, 3
Leishmania braziliensis LbrM.28.0120   proteasome beta 3 subunit, putative
Leishmania donovani LdBPK_280110.1   proteasome beta 3 subunit, putative
Leishmania infantum LinJ.28.0110   proteasome beta 3 subunit, putative
Leishmania major LmjF.28.0110   proteasome beta 3 subunit, putative
Leishmania mexicana LmxM.28.0110   proteasome beta 3 subunit, putative
Loa Loa (eye worm) LOAG_01409   proteasome subunit beta type 3
Mus musculus ENSMUSG00000069744   proteasome (prosome, macropain) subunit, beta type 3
Neospora caninum NCLIV_057270   hypothetical protein
Oryza sativa 4328517   Os02g0182500
Oryza sativa 4341637   Os06g0643100
Plasmodium berghei PBANKA_0205400   proteasome subunit beta type-3, putative
Plasmodium falciparum PF3D7_0108000   proteasome subunit beta type-3, putative
Plasmodium knowlesi PKNH_0205600   proteasome subunit beta type-3, putative
Plasmodium vivax PVX_081375   proteasome subunit beta type-3, putative
Plasmodium yoelii PY06176   7006-8626
Saccharomyces cerevisiae YER094C   proteasome core particle subunit beta 3
Schistosoma japonicum Sjp_0305610   ko:K02735 20S proteasome subunit beta 3, putative
Schistosoma mansoni Smp_121430.2   proteasome subunit beta 3 (T01 family)
Schistosoma mansoni Smp_121430.1   proteasome subunit beta 3 (T01 family)
Schmidtea mediterranea mk4.000241.05   Proteasome subunit beta type-3
Trypanosoma brucei gambiense Tbg972.11.8310   proteasome beta 3 subunit, putative
Trypanosoma brucei Tb927.11.7270   proteasome beta 3 subunit, putative
Trypanosoma congolense TcIL3000_0_00860   proteasome beta 3 subunit, putative
Trypanosoma congolense TcIL3000_0_05230   proteasome beta 3 subunit, putative
Trypanosoma cruzi TcCLB.506779.50   proteasome beta 3 subunit, putative
Trypanosoma cruzi TcCLB.511153.140   proteasome beta 3 subunit, putative
Toxoplasma gondii TGME49_314090   proteasome beta subunit
Theileria parva TP04_0618   proteasome subunit beta type 3, putative
Trichomonas vaginalis TVAG_286960   Family T1, proteasome beta subunit, threonine peptidase

Essentiality

Tb927.11.7270 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.5170 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.5170 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.5170 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.5170 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y38A8.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y38A8.2 Caenorhabditis elegans larval lethal wormbase
CELE_Y38A8.2 Caenorhabditis elegans slow growth wormbase
CELE_Y38A8.2 Caenorhabditis elegans sterile wormbase
YER094C Saccharomyces cerevisiae inviable yeastgenome
TGME49_314090 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) subunit, beta type, 3 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0123 0.3165 0.4641
0.0247 0.3303 0.4769
0.0111 0.2624 0.4641
0.0224 0.3179 0.4658
0.0134 0.3179 0.4658
0.0134 0.3165 0.4641
0.0233 0.3179 0.4658
0.0134 0.3179 0.4658
0.013 0.3153 0.4628
0.0108 0.3164 0.4641
0.0134 0.3165 0.4641
0.012 0.3374 0.3529
0.0254 0.3165 0.4658
0.0134 0.3165 0.4641
0.0249 0.3167 0.4658
0.0242 0.3179 0.4658
0.0134 0.3165 0.4641
0.0117 0.3377 0.3509

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 2 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.11.7270 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 2 )

Bibliographic References

35 literature references were collected for this gene.

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Gene identifier Tb927.11.7270 (Trypanosoma brucei), proteasome beta 3 subunit, putative
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