Detailed view for Tb927.10.13630

Basic information

TDR Targets ID: 15100
Trypanosoma brucei, glucosidase, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 6.1466 | Length (AA): 807 | MW (Da): 91490 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01055   Glycosyl hydrolases family 31
PF13802   Galactose mutarotase-like

Gene Ontology

Mouse over links to read term descriptions.
GO:0004553   hydrolase activity, hydrolyzing O-glycosyl compounds  
GO:0030246   carbohydrate binding  
GO:0003824   catalytic activity  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 805 3l4y (A) 65 868 26.00 0 1 1.31557 -0.35
7 805 3lpp (A) 95 898 24.00 0 1 1.28809 -0.3
31 806 5f0e (A) 89 966 40.00 0 1 1.48659 -0.97

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126880)

Species Accession Gene Product
Arabidopsis thaliana AT3G23640   heteroglycan glucosidase 1
Arabidopsis thaliana AT5G63840   alpha 1,3-glucosidase
Brugia malayi Bm1_51425   Glycosyl hydrolases family 31 protein
Candida albicans CaO19.974   similar to S. cerevisiae ROT2 (YBR229C) ER alpha-glucosidase involved in cell wall biosynthesis
Candida albicans CaO19.8589   similar to S. cerevisiae ROT2 (YBR229C) ER alpha-glucosidase involved in cell wall biosynthesis
Caenorhabditis elegans CELE_F40F9.6   Protein AAGR-3, isoform C
Caenorhabditis elegans CELE_F52D1.1   Protein AAGR-4
Cryptosporidium hominis Chro.80167   hypothetical protein
Cryptosporidium hominis Chro.30190   glucosidase II
Cryptosporidium parvum cgd3_1580   alpha glucosidase-like faimly 31 glycosyl hydrolases
Cryptosporidium parvum cgd8_1420   secreted alpha glucosidase like family 31 glycosyltransferase, signal peptide
Dictyostelium discoideum DDB_G0269154   alpha-glucosidase II
Drosophila melanogaster Dmel_CG14476   CG14476 gene product from transcript CG14476-RC
Escherichia coli b3656   putative alpha-glucosidase
Echinococcus granulosus EgrG_000716600   neutral alpha glucosidase AB
Entamoeba histolytica EHI_023430   glycosyl hydrolase, family 31 protein
Echinococcus multilocularis EmuJ_000716600   neutral alpha glucosidase AB
Homo sapiens 2595   glucosidase, alpha
Homo sapiens ENSG00000089597   glucosidase, alpha
Leishmania braziliensis LbrM.18.0110   alpha glucosidase II subunit, putative
Leishmania donovani LdBPK_180090.1   alpha glucosidase II subunit, putative
Leishmania infantum LinJ.18.0090   alpha glucosidase II subunit, putative
Leishmania major LmjF.18.0090   alpha glucosidase II subunit, putative
Leishmania mexicana LmxM.18.0090   alpha glucosidase II subunit, putative
Loa Loa (eye worm) LOAG_00724   glycosyl hydrolase family 31 protein
Mus musculus 76051   glucosidase, alpha
Mus musculus ENSMUSG00000071650   alpha glucosidase 2 alpha neutral subunit
Neospora caninum NCLIV_007580   hypothetical protein
Oryza sativa 4343045   Os07g0421300
Oryza sativa 4343041   Os07g0420700
Oryza sativa 4332068   Os03g0216600
Saccharomyces cerevisiae YBR229C   glucan 1,3-alpha-glucosidase ROT2
Schistosoma japonicum Sjp_0302120   Neutral alpha-glucosidase AB precursor, putative
Schistosoma japonicum Sjp_0043610   ko:K05546 alpha 1,3-glucosidase [EC3.2.1.84], putative
Schistosoma mansoni Smp_018760   alpha glucosidase
Schmidtea mediterranea mk4.006001.00  
Schmidtea mediterranea mk4.000318.02   Neutral alpha-glucosidase C
Schmidtea mediterranea mk4.003877.03  
Schmidtea mediterranea mk4.006001.01   Neutral alpha-glucosidase AB
Schmidtea mediterranea mk4.003877.01   Neutral alpha-glucosidase AB
Schmidtea mediterranea mk4.026406.00   Neutral alpha-glucosidase C
Trypanosoma brucei gambiense Tbg972.10.16730   glucosidase, putative
Trypanosoma brucei Tb927.10.13630   glucosidase, putative
Trypanosoma congolense TcIL3000_10_13840   glucosidase, putative
Trypanosoma cruzi TcCLB.511279.60   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507993.260   hypothetical protein, conserved
Toxoplasma gondii TGME49_253030   glycosyl hydrolase, family 31 protein
Trichomonas vaginalis TVAG_337640   neutral alpha-glucosidase ab precursor, putative
Trichomonas vaginalis TVAG_203340   alpha-glucosidase, putative
Trichomonas vaginalis TVAG_379190   alpha-glucosidase, putative
Trichomonas vaginalis TVAG_225550   alpha-glucosidase, putative
Trichomonas vaginalis TVAG_126630   neutral alpha-glucosidase ab precursor, putative
Trichomonas vaginalis TVAG_047190   alpha-glucosidase, putative
Trichomonas vaginalis TVAG_465150   maltase-glucoamylase, putative
Trichomonas vaginalis TVAG_273960   alpha-glucosidase, putative

Essentiality

Tb927.10.13630 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.13630 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.13630 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.13630 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.13630 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3656 Escherichia coli non-essential goodall
CELE_F40F9.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_F40F9.6 Caenorhabditis elegans larval arrest wormbase
TGME49_253030 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens glucosidase, alpha Compounds References
Homo sapiens glucosidase, alpha Compounds References
Mus musculus glucosidase, alpha Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0347 0.3033 1
0.0352 0.3014 1
0.1881 0.2737 1
0.1995 0.2588 1
0.0444 0.3026 1
0.0438 0.2862 1
0.0231 0.3658 1
0.0321 0.2966 1
0.2419 0.2662 0.7135
0.2207 0.2572 1
0.0352 0.3014 1
0.0346 0.2798 1
0.0377 0.2846 1
0.0346 0.2798 1
0.039 0.3007 1
0.0154 0.303 1
0.0564 0.2917 1
0.2419 0.2662 0.7135
0.1995 0.2588 1
0.2118 0.2601 1
0.1021 0.2676 1
0.2481 0.254 1
0.2481 0.254 1
0.0628 0.305 1
0.2518 0.2583 1
0.1416 0.2897 1
0.1045 0.2523 1
0.1045 0.2523 1
0.1018 0.259 1
0.0352 0.3014 1
0.0209 0.3658 1
0.0385 0.3016 1
0.0427 0.3309 1
0.2814 0.2516 1
0.0391 0.3006 1
0.2481 0.254 1
0.0876 0.2667 1
0.0564 0.2917 1
0.1021 0.2676 1
0.0564 0.2917 1
0.0243 0.29 0.4412
0.0194 0.2736 1
0.2287 0.2639 1
0.1018 0.259 1
0.0292 0.2965 1

Assayability

Assay information

  • Assay for Maltase (3.2.1.20 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

5 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.10.13630 (Trypanosoma brucei), glucosidase, putative
Title for this comment
Comment