Detailed view for Tb927.8.3530

Basic information

TDR Targets ID: 15247
Trypanosoma brucei, glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.8435 | Length (AA): 354 | MW (Da): 37805 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01210   NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
PF07479   NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus

Gene Ontology

Mouse over links to read term descriptions.
GO:0020015   glycosome  
GO:0016616   oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor  
GO:0004367   glycerol-3-phosphate dehydrogenase (NAD+) activity  
GO:0009331   glycerol-3-phosphate dehydrogenase complex  
GO:0005737   cytoplasm  
GO:0051287   NAD binding  
GO:0050662   coenzyme binding  
GO:0042803   protein homodimerization activity  
GO:0016491   oxidoreductase activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0055114   oxidation reduction  
GO:0046168   glycerol-3-phosphate catabolic process  
GO:0008152   metabolic process  
GO:0006072   glycerol-3-phosphate metabolic process  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126704)

Species Accession Gene Product
Arabidopsis thaliana AT5G40610   glycerol-3-phosphate dehydrogenase [NAD+]
Arabidopsis thaliana AT2G40690   glycerol-3-phosphate dehydrogenase [NAD(+)] 2
Babesia bovis BBOV_IV004480   glycerol-3-phosphate dehydrogenase, putative
Brugia malayi Bm1_45570   Probable glycerol-3-phosphate dehydrogenase, cytoplasmic
Candida albicans CaO19.8310   one of two genes similar to S. cerevisiae GPD1 (YDL022W) and GPD2 (YOL059W) glycerol-3-phosphate dehydrogenase
Candida albicans CaO19.9325   one of two genes similar to S. cerevisiae GPD1 (YDL022W) and GPD2 (YOL059W) glycerol-3-phosphate dehydrogenase
Candida albicans CaO19.691   one of two genes similar to S. cerevisiae GPD1 (YDL022W) and GPD2 (YOL059W) glycerol-3-phosphate dehydrogenase
Candida albicans CaO19.1756   one of two genes similar to S. cerevisiae GPD1 (YDL022W) and GPD2 (YOL059W) glycerol-3-phosphate dehydrogenase
Caenorhabditis elegans CELE_F47G4.3   Protein GPDH-1
Caenorhabditis elegans CELE_K11H3.1   Protein GPDH-2, isoform C
Cryptosporidium hominis Chro.20028   glycerol-3-phosphate dehydrogenase
Cryptosporidium parvum cgd2_210   glycerol-3-phosphate dehydrogenase
Chlamydia trachomatis CT_714   glycerol-3-phosphate dehydrogenase
Drosophila melanogaster Dmel_CG3215   CG3215 gene product from transcript CG3215-RA
Drosophila melanogaster Dmel_CG9042   Glycerol 3 phosphate dehydrogenase
Escherichia coli b3608   glycerol-3-phosphate dehydrogenase (NAD+)
Echinococcus granulosus EgrG_000686100   glycerol 3 phosphate dehydrogenase NAD
Echinococcus multilocularis EmuJ_000686100   glycerol 3 phosphate dehydrogenase (NAD+)
Homo sapiens ENSG00000152642   glycerol-3-phosphate dehydrogenase 1-like
Homo sapiens ENSG00000167588   glycerol-3-phosphate dehydrogenase 1 (soluble)
Leishmania braziliensis LbrM.10.0640   glycerol-3-phosphate dehydrogenase [NAD ], glycosomal
Leishmania donovani LdBPK_100560.1   glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
Leishmania infantum LinJ.10.0560   glycerol-3-phosphate dehydrogenase [NAD ], glycosomal
Leishmania major LmjF.10.0510   glycerol-3-phosphate dehydrogenase [NAD ], glycosomal
Leishmania mexicana LmxM.10.0510   glycerol-3-phosphate dehydrogenase [NAD ], glycosomal/mitochondrial
Loa Loa (eye worm) LOAG_00716   glycerol-3-Phosphate DeHydrogenase family member
Loa Loa (eye worm) LOAG_11450   glycerol 3 phosphate dehydrogenase
Loa Loa (eye worm) LOAG_09844   hypothetical protein
Mycobacterium leprae ML1679c   PROBABLE GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(P)+] GPDA (NAD(P)H- DEPENDENT GLYCEROL-3-PHOSPHATE DEHYDROGENASE)
Mus musculus ENSMUSG00000023019   glycerol-3-phosphate dehydrogenase 1 (soluble)
Mus musculus ENSMUSG00000050627   glycerol-3-phosphate dehydrogenase 1-like
Mycobacterium tuberculosis Rv0564c   Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA1 (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) (NAD(P)H-depe
Mycobacterium tuberculosis Rv2982c   Probable glycerol-3-phosphate dehydrogenase [NAD(P)+] GpdA2 (NAD(P)H- dependent glycerol-3-phosphate dehydrogenase)
Mycobacterium ulcerans MUL_1975   NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
Mycobacterium ulcerans MUL_0667   NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
Neospora caninum NCLIV_043550   Glycerol-3-phosphate dehydrogenase, related
Neospora caninum NCLIV_001180   CBR-GPDH-1 protein, related
Oryza sativa 4342770   Os07g0229800
Onchocerca volvulus OVOC3115  
Onchocerca volvulus OVOC8373  
Plasmodium berghei PBANKA_0932900   glycerol-3-phosphate dehydrogenase, putative
Plasmodium berghei PBANKA_1431900   glycerol-3-phosphate dehydrogenase, putative
Plasmodium falciparum PF3D7_1216200   glycerol-3-phosphate dehydrogenase, putative
Plasmodium falciparum PF3D7_1114800   glycerol-3-phosphate dehydrogenase, putative
Plasmodium knowlesi PKNH_0912300   glycerol-3-phosphate dehydrogenase, putative
Plasmodium knowlesi PKNH_1435700   glycerol-3-phosphate dehydrogenase, putative
Plasmodium vivax PVX_091380   glycerol-3-phosphate dehydrogenase, putative
Plasmodium vivax PVX_123490   glycerol-3-phosphate dehydrogenase, putative
Plasmodium yoelii PY02925   Unknown-related
Plasmodium yoelii PY00789   NAD-dependent glycerol-3-phosphate dehydrogenase
Plasmodium yoelii PY05585   glycerol-3-phosphate dehydrogenase
Saccharomyces cerevisiae YDL022W   glycerol-3-phosphate dehydrogenase (NAD(+)) GPD1
Saccharomyces cerevisiae YOL059W   glycerol-3-phosphate dehydrogenase (NAD(+)) GPD2
Schistosoma japonicum Sjp_0005480   ko:K00006 glycerol-3-phosphate dehydrogenase (NAD+) [EC1.1.1.8], putative
Schistosoma japonicum Sjp_0117170   Glycerol-3-phosphate dehydrogenase [NAD+], putative
Schistosoma mansoni Smp_030500.1   glycerol-3-phosphate dehydrogenase
Schistosoma mansoni Smp_030500.2   glycerol-3-phosphate dehydrogenase
Schmidtea mediterranea mk4.008015.01   Glycerol-3-phosphate dehydrogenase, putative
Schmidtea mediterranea mk4.001050.01  
Schmidtea mediterranea mk4.001797.04  
Schmidtea mediterranea mk4.001020.06  
Schmidtea mediterranea mk4.000878.05  
Schmidtea mediterranea mk4.011976.00  
Schmidtea mediterranea mk4.001612.07  
Schmidtea mediterranea mk4.003385.00  
Schmidtea mediterranea mk4.002551.01  
Schmidtea mediterranea mk4.000946.04  
Schmidtea mediterranea mk4.007040.01  
Schmidtea mediterranea mk4.014739.01  
Schmidtea mediterranea mk4.003527.01  
Schmidtea mediterranea mk4.002903.00  
Schmidtea mediterranea mk4.009142.03  
Schmidtea mediterranea mk4.025464.00  
Schmidtea mediterranea mk4.006553.00   Glycerol-3-phosphate dehydrogenase, putative
Trypanosoma brucei gambiense Tbg972.8.3210   glycerol-3-phosphate dehydrogenase [NAD ], glycosomal, putative
Trypanosoma brucei Tb927.8.3530   glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
Trypanosoma congolense TcIL3000_8_3450   glycerol-3-phosphate dehydrogenase [NAD+], glycosomal, putative
Toxoplasma gondii TGME49_307570   glycerol-3-phosphate dehydrogenase (gpdh), putative
Toxoplasma gondii TGME49_210260   NAD-dependent glycerol-3-phosphate dehydrogenase
Treponema pallidum TP1009   NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
Theileria parva TP01_0302   NAD-dependent glycerol-3-phosphate dehydrogenase, putative
Trichomonas vaginalis TVAG_586980   glycerol-3-phosphate dehydrogenase, putative
Trichomonas vaginalis TVAG_601920   glycerol-3-phosphate dehydrogenase, putative
Trichomonas vaginalis TVAG_230610   glycerol-3-phosphate dehydrogenase, putative
Trichomonas vaginalis TVAG_525580   glycerol-3-phosphate dehydrogenase, putative
Trichomonas vaginalis TVAG_382880   glycerol-3-phosphate dehydrogenase, putative
Trichomonas vaginalis TVAG_361130   glycerol-3-phosphate dehydrogenase, putative
Trichomonas vaginalis TVAG_304500   glycerol-3-phosphate dehydrogenase, putative
Trichomonas vaginalis TVAG_512070   glycerol-3-phosphate dehydrogenase, putative
Trichomonas vaginalis TVAG_386800   glycerol-3-phosphate dehydrogenase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0597   NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

Essentiality

Tb927.8.3530 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu572 Mycobacterium tuberculosis non-essential nmpdr
mtu3032 Mycobacterium tuberculosis non-essential nmpdr
Tb927.8.3530 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.3530 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.3530 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.3530 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3608 Escherichia coli essential goodall
PBANKA_0932900 Plasmodium berghei Dispensable plasmo
PBANKA_1431900 Plasmodium berghei Dispensable plasmo
TGME49_307570 Toxoplasma gondii Essentiality uncertain sidik
TGME49_210260 Toxoplasma gondii Essentiality uncertain sidik
TGME49_307570 Toxoplasma gondii Probably essential sidik
TGME49_210260 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

  • Validation: inhibited in cell-free system. As observed in: T. b. brucei.Evidence: inferred from specific protein inhibition
  • annotated by: ts4@sanger.ac.uk.
  • References: 11512153

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for α-Glycerophosphate Dehydrogenase (1.1.1.8 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 3 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.8.3530 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 3 )

Bibliographic References

8 literature references were collected for this gene.

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Gene identifier Tb927.8.3530 (Trypanosoma brucei), glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
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