Detailed view for Tb927.11.10450

Basic information

TDR Targets ID: 15349
Trypanosoma brucei, UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.3238 | Length (AA): 393 | MW (Da): 43114 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 10

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00953   Glycosyl transferase family 4

Gene Ontology

Mouse over links to read term descriptions.
GO:0006488   dolichol-linked oligosaccharide biosynthetic process  
GO:0003975   UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity  
GO:0016021   integral to membrane  
GO:0008963   phospho-N-acetylmuramoyl-pentapeptide-transferase activity  
GO:0006487   protein amino acid N-linked glycosylation  

Metabolic Pathways

N-Glycan biosynthesis (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
21 385 5lev (A) 10 402 37.00 0 0.96 1.16315 -0.24
42 190 4ryo (A) 7 152 14.00 0.8 0.15 0.541535 -0.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127672)

Species Accession Gene Product
Arabidopsis thaliana AT3G57220   Glycosyl transferase family 4 protein
Arabidopsis thaliana AT2G41490   UDP-GlcNAc:dolichol phosphate N-acetylglucosamine-1-phosphate transferase
Babesia bovis BBOV_IV000950   N-acetylglucosamine-1-phosphate transferase
Brugia malayi Bm1_52335   UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase
Candida albicans CaO19.2187   UDP-GlcNAc-1-P transferase
Candida albicans CaO19.9733   UDP-GlcNAc-1-P transferase
Caenorhabditis elegans CELE_Y60A3A.14   Protein Y60A3A.14
Caenorhabditis elegans CELE_D1005.2   Protein D1005.2
Cryptosporidium hominis Chro.50156   N-acetylglucosamine-1-phosphate transferase
Cryptosporidium parvum cgd5_2240   DPAGT1 like N-acetylglucosamine-1-phosphate transferase
Drosophila melanogaster Dmel_CG5287   CG5287 gene product from transcript CG5287-RA
Echinococcus granulosus EgrG_000941900   UDP N acetylglucosamine dolichyl phosphate
Entamoeba histolytica EHI_062840   UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Echinococcus multilocularis EmuJ_000941900   UDP N acetylglucosamine dolichyl phosphate
Giardia lamblia GL50803_15889   UDP-N-acetylglucosamine-dolichyl-phosphateN-acetylglucosaminephosphotransferase
Homo sapiens ENSG00000172269   dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
Leishmania braziliensis LbrM.35.4430   UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Leishmania donovani LdBPK_364390.1   UDP-N-acetylglucosamine--dolichyl-phosphaten-ac et ylglucosaminephosphotransferase
Leishmania infantum LinJ.36.4390   UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Leishmania major LmjF.36.4180   UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Leishmania mexicana LmxM.36.4180   UDP-N-acetylglucosamine--dolichyl-phosphaten-ac et ylglucosaminephosphotransferase
Loa Loa (eye worm) LOAG_01182   hypothetical protein
Mus musculus ENSMUSG00000032123   dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
Neospora caninum NCLIV_019070   Os07g0661100 protein, related
Oryza sativa 4344180   Os07g0661100
Onchocerca volvulus OVOC6591  
Plasmodium berghei PBANKA_1217300   UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Plasmodium falciparum PF3D7_0321200   UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Plasmodium knowlesi PKNH_0819600   UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Plasmodium vivax PVX_095175   N-acetylglucosamine-1-phosphate transferase, putative
Plasmodium yoelii PY00432   N-acetylglucosamine-1-phosphate transferase
Saccharomyces cerevisiae YBR243C   UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Schistosoma japonicum Sjp_0210240   ko:K01001 UDP-N-acetylglucosamine--dolichyl-phosphate [EC2.7.8.15], putative
Schistosoma mansoni Smp_051360.1   UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Schistosoma mansoni Smp_051360.2   UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Schmidtea mediterranea mk4.016533.00   UDP-N-acetylglucosamineALQ-dolichyl-phosphate N-acetylglucosaminephosphotransferase
Schmidtea mediterranea mk4.009878.02   UDP-N-acetylglucosamineALQ-dolichyl-phosphate N-acetylglucosaminephosphotransferase
Trypanosoma brucei gambiense Tbg972.11.11710   UDP-N-acetylglucosamine--dolichyl-phosphate n- acetylglucosaminephosphotransferase, putative
Trypanosoma brucei Tb927.11.10450   UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Trypanosoma congolense TcIL3000.11.11010   UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Trypanosoma cruzi TcCLB.510283.140   UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Trypanosoma cruzi TcCLB.503823.104   UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
Toxoplasma gondii TGME49_244520   glycosyl transferase, group 4 family protein
Theileria parva TP01_0118   N-acetylglucosamine-1-phosphate transferase, putative
Trichomonas vaginalis TVAG_308000   glucosaminephosphotransferase, putative

Essentiality

Tb927.11.10450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.2220 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.2220 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.2220 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.2220 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YBR243C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1217300 Plasmodium berghei Essential plasmo
TGME49_244520 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.10450 (Trypanosoma brucei), UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase, putative
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