Detailed view for Tb927.8.4040

Basic information

TDR Targets ID: 15384
Trypanosoma brucei, endonuclease G, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.3074 | Length (AA): 506 | MW (Da): 55838 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 1

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01223   DNA/RNA non-specific endonuclease

Gene Ontology

Mouse over links to read term descriptions.
GO:0006139   nucleobase, nucleoside, nucleotide and nucleic acid metabolic process  
GO:0005739   mitochondrion  
GO:0046872   metal ion binding  
GO:0016787   hydrolase activity  
GO:0004519   endonuclease activity  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128153)

Species Accession Gene Product
Brugia malayi Bm1_04330   endonuclease G, mitochondrial precursor
Candida albicans CaO19.967   Mitochondrial nuclease
Candida albicans CaO19.8582   Mitochondrial nuclease
Caenorhabditis elegans CELE_C41D11.8   Protein CPS-6
Cryptosporidium hominis Chro.50161   endonuclease G-like 1
Cryptosporidium parvum cgd5_2190   Nuc1p like endonuclease G
Dictyostelium discoideum DDB_G0287551   hypothetical protein
Drosophila melanogaster Dmel_CG32463   Testis EndoG-Like 2
Drosophila melanogaster Dmel_CG8862   Endonuclease G
Echinococcus granulosus EgrG_000100500   Endonuclease G
Echinococcus multilocularis EmuJ_000100500   Endonuclease G
Homo sapiens ENSG00000167136   endonuclease G
Homo sapiens ENSG00000157036   endo/exonuclease (5'-3'), endonuclease G-like
Leishmania braziliensis LbrM.10.0750   endonuclease G, putative
Leishmania donovani LdBPK_100660.1   endonuclease G, putative
Leishmania infantum LinJ.10.0660   endonuclease G, putative
Leishmania major LmjF.10.0610   endonuclease G, putative
Leishmania mexicana LmxM.10.0610   endonuclease G, putative
Mus musculus ENSMUSG00000015337   endonuclease G
Mus musculus ENSMUSG00000042787   endo/exonuclease (5'-3'), endonuclease G-like
Neospora caninum NCLIV_003230   DNA/RNA non-specific endonuclease domain- containing protein, putative
Saccharomyces cerevisiae YJL208C   Nuc1p
Schistosoma japonicum Sjp_0092300   IPR001604,DNA/RNA non-specific endonuclease,domain-containing
Schistosoma japonicum Sjp_0120210   Endonuclease G, mitochondrial precursor, putative
Schistosoma japonicum Sjp_0105780   IPR001604,DNA/RNA non-specific endonuclease,domain-containing
Schistosoma japonicum Sjp_0057710   ko:K01173 endonuclease [EC3.1.30.-], putative
Schistosoma mansoni Smp_105080   endonuclease related
Schmidtea mediterranea mk4.002455.05   Endonuclease G, mitochondrial
Trypanosoma brucei gambiense Tbg972.8.3790   endonuclease G, putative
Trypanosoma brucei Tb927.8.4040   endonuclease G, putative
Trypanosoma brucei Tb11.v5.0860   endonuclease G, putative
Trypanosoma brucei Tb927.8.4090   endonuclease G, putative
Trypanosoma congolense TcIL3000_0_06670   endonuclease G, putative
Trypanosoma cruzi TcCLB.506867.10   endonuclease G, putative
Trypanosoma cruzi TcCLB.507623.140   endonuclease G, putative
Toxoplasma gondii TGME49_208710   DNA/RNA non-specific endonuclease

Essentiality

Tb927.8.4040 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.4040 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.4040 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.4040 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.4040 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_208710 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0108 0.2614 1
0.0314 0.2677 1
0.0263 0.2748 1
0.0303 0.267 0.8979
0.0147 0.2695 1
0.031 0.2753 1
0.027 0.2656 1
0.0208 0.264 1
0.035 0.2701 1
0.0217 0.2723 1
0.0106 0.2702 1
0.0108 0.2614 1
0.034 0.2673 1
0.0106 0.2662 1
0.0346 0.2688 1
0.0453 0.2702 1
0.0359 0.2735 1
0.0236 0.254 1
0.0401 0.2729 1
0.0125 0.2679 1
0.0311 0.2668 1
0.0196 0.2684 0.9211
0.0335 0.2671 1
0.0407 0.2712 1
0.0385 0.2711 1
0.0329 0.2755 1
0.0149 0.2626 1
0.0319 0.2682 1
0.027 0.2656 1
0.0127 0.2677 1
0.0104 0.2658 1
0.0263 0.2748 1
0.0209 0.2661 1
0.0242 0.2564 1
0.0212 0.2553 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.8.4040 (Trypanosoma brucei), endonuclease G, putative
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