Detailed view for Tb927.8.5730

Basic information

TDR Targets ID: 15686
Trypanosoma brucei, STE20-like serine/threonine-protein kinase 1, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.4363 | Length (AA): 473 | MW (Da): 52966 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 280 1u5q (A) 12 288 41.00 0 1 1.05674 -0.96
13 294 4usf (A) 27 310 46.00 0 1 1.16819 -0.88
15 320 4lgd (A) 22 446 44.00 0 1 1.20623 -0.71
15 289 2x7f (E) 20 307 49.00 0 1 1.0484 -0.53
20 395 4z7g (A) 571 960 17.00 0 1 0.905226 0.47

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128604)

Species Accession Gene Product
Arabidopsis thaliana AT1G69220   putative serine/threonine kinase
Brugia malayi Bm1_32140   STE20-like kinase MST
Candida albicans CaO19.3049   kinase required for spore wall formation
Candida albicans CaO19.10567   kinase required for spore wall formation
Caenorhabditis elegans CELE_F14H12.4   Protein CST-1, isoform B
Caenorhabditis elegans CELE_C24A8.4   Protein CST-2
Cryptosporidium hominis Chro.70160   serine/threonine protein kinase; possibly sporulation specific
Dictyostelium discoideum DDB_G0274593   STE20 family protein kinase
Dictyostelium discoideum DDB_G0284181   MST subfamily kinase
Drosophila melanogaster Dmel_CG11228   hippo
Echinococcus granulosus EgrG_000067500   serine:threonine protein kinase 3
Entamoeba histolytica EHI_156670   serine/threonine-protein kinase 3, putative
Entamoeba histolytica EHI_192540   serine/threonine protein kinase STE20, putative
Echinococcus multilocularis EmuJ_000364700   myosin iiia
Echinococcus multilocularis EmuJ_000067500   serine:threonine protein kinase 3
Giardia lamblia GL50803_15514   Kinase, STE STE20
Homo sapiens ENSG00000101109   serine/threonine kinase 4
Homo sapiens ENSG00000104375   serine/threonine kinase 3
Leishmania braziliensis LbrM.16.0300   protein kinase, putative
Leishmania donovani LdBPK_160310.1   protein kinase, putative
Leishmania infantum LinJ.16.0310   protein kinase, putative
Leishmania major LmjF.16.0300   protein kinase, putative
Leishmania mexicana LmxM.16.0300   protein kinase, putative
Loa Loa (eye worm) LOAG_07747   STE/STE20/MST protein kinase
Mus musculus ENSMUSG00000018209   serine/threonine kinase 4
Mus musculus ENSMUSG00000022329   serine/threonine kinase 3
Oryza sativa 4334150   Os03g0755000
Plasmodium knowlesi PKNH_0418000   protein kinase, putative
Schmidtea mediterranea mk4.011996.00   Serine/threonine-protein kinase flr-4
Schmidtea mediterranea mk4.010105.01  
Schmidtea mediterranea mk4.009495.01  
Schmidtea mediterranea mk4.000035.12  
Trypanosoma brucei gambiense Tbg972.8.5730   protein kinase, putative
Trypanosoma brucei Tb927.8.5730   STE20-like serine/threonine-protein kinase 1, putative
Trypanosoma congolense TcIL3000_8_5520   protein kinase, putative
Trypanosoma cruzi TcCLB.506743.160   STE/STE20 serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.503473.10   STE/STE20 serine/threonine-protein kinase, putative
Trichomonas vaginalis TVAG_268810   STE family protein kinase

Essentiality

Tb927.8.5730 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5730 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5730 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.5730 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.5730 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.9

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mus musculus serine/threonine kinase 3 Compounds References
Homo sapiens serine/threonine kinase 3 Compounds References
Homo sapiens serine/threonine kinase 4 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 33.5% 161 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 28.5% 288 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 28.7% 300 aa Compounds References
Zea mays Casein kinase II alpha 332 aa 25.9% 294 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0059 1 1
0.0011 1 0.5
0.0008 0.5 0.5
0.0027 1 0.5
0.0036 0.5 0.5
0.0012 0.5 0.5
0.0022 0.5 0.5
0.0056 1 0.5
0.0066 0.3101 0
0.0029 0.5 0.5
0.0012 0.5 0.5
0.0067 0.5 0.5
0.0061 0.6883 0.5304
0.0023 0.5 0.5
0.0004 0.5 0.5
0.0092 1 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0375079 0.256497 1
0.0042 0.5 0.5
0.0016 0.5 0.5
0.0063 1 0.5
0.0012 0.5 0.5
0.0037 1 0.5
0.0059 1 1
0.0064 0.3377 0
0.0039 0.5 0.5
0.0003 0.5 0.5
0.0033 1 0.5
0.0016 0.5 0.5
0.0007 0.5 0.5
0.0018 0.5 0.5
0.00673621 0.250986 1
0.0091 1 0.5
0.0081 0.5 0.5
0.0032 0.5 0.5
0.0088 0.4477 0.5
0.0093 0.8828 0
0.0081 1 0.5
0.0033 0.5 0.5
0.0026 0.5 0.5
0.0098 0.3242 0.2614
0.0007 0.5 0.5
0.0069 0.3067 1
0.0062 0.6935 0
0.0059 1 1
0.0063 0.7244 0.2543
0.0039 0.9485 0.5
0.0007 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.8.5730 (Trypanosoma brucei), STE20-like serine/threonine-protein kinase 1, putative
Title for this comment
Comment