Detailed view for Tb927.11.7020

Basic information

TDR Targets ID: 15953
Trypanosoma brucei, proteasome alpha 7 subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.9651 | Length (AA): 247 | MW (Da): 27865 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00227   Proteasome subunit
PF10584   Proteasome subunit A N-terminal signature

Gene Ontology

Mouse over links to read term descriptions.
GO:0019773   proteasome core complex, alpha-subunit complex  
GO:0005829   cytosol  
GO:0005839   proteasome core complex  
GO:0005634   nucleus  
GO:0000502   proteasome complex  
GO:0004298   threonine endopeptidase activity  
GO:0004175   endopeptidase activity  
GO:0051603   proteolysis involved in cellular protein catabolic process  
GO:0006511   ubiquitin-dependent protein catabolic process  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 240 1j2p (A) 6 233 43.00 0 1 1.52026 -0.48
2 235 5le5 (C) 2 238 58.00 0 1 1.70997 -1.02

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 1FNT:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 1Z7Q:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127424)

Species Accession Gene Product
Arabidopsis thaliana AT3G51260   20S proteasome alpha subunit PAD1
Arabidopsis thaliana AT5G66140   proteasome alpha subunit D2
Babesia bovis BBOV_III009200   proteasome A-type and B-type family protein
Brugia malayi Bm1_49465   Proteasome subunit alpha type 7-1
Caenorhabditis elegans CELE_C36B1.4   Protein PAS-4
Cryptosporidium hominis Chro.20158   proteasome subunit alpha type 7 (Proteasome component DD5)
Cryptosporidium parvum cgd2_1440   putative proteasome regulatory subunit, NTN hydrolase fold
Dictyostelium discoideum DDB_G0272831   20S proteasome subunit alpha-7
Drosophila melanogaster Dmel_CG3422   Proteasome alpha4 subunit
Echinococcus granulosus EgrG_000682700   Proteasome subunit alpha type 7
Entamoeba histolytica EHI_090000   proteasome subunit alpha type 7, putative
Entamoeba histolytica EHI_163650   proteasome subunit alpha type 7, putative
Echinococcus multilocularis EmuJ_000682700   Proteasome subunit alpha type 7
Echinococcus multilocularis EmuJ_000682300   Proteasome subunit alpha type 7
Echinococcus multilocularis EmuJ_000682550   Proteasome subunit alpha type 7
Giardia lamblia GL50803_15099   20S proteasome alpha subunit 4
Homo sapiens ENSG00000154611   proteasome (prosome, macropain) subunit, alpha type, 8
Homo sapiens ENSG00000101182   proteasome (prosome, macropain) subunit, alpha type, 7
Leishmania braziliensis LbrM.19.0080   proteasome alpha 7 subunit, putative
Leishmania donovani LdBPK_110240.1   proteasome alpha 7 subunit, putative
Leishmania infantum LinJ.11.0240   proteasome alpha 7 subunit, putative
Leishmania major LmjF.11.0240   proteasome alpha 7 subunit, putative
Leishmania mexicana LmxM.11.0240   proteasome alpha 7 subunit, putative
Loa Loa (eye worm) LOAG_01577   proteasome subunit alpha type 7-1
Mus musculus ENSMUSG00000036743   proteasome (prosome, macropain) subunit, alpha type, 8
Mus musculus ENSMUSG00000027566   proteasome (prosome, macropain) subunit, alpha type 7
Neospora caninum NCLIV_028230   Proteasome subunit alpha type-7, related
Oryza sativa 4346245   Os08g0548900
Oryza sativa 4347710   Os09g0538200
Plasmodium berghei PBANKA_1130500   proteasome subunit alpha type-7, putative
Plasmodium falciparum PF3D7_1353900   proteasome subunit alpha type-7, putative
Plasmodium knowlesi PKNH_1117800   proteasome subunit alpha type-7, putative
Plasmodium vivax PVX_114685   proteasome subunit alpha type-7, putative
Plasmodium yoelii PY02351   Y13180 multicatalytic endopeptidase
Saccharomyces cerevisiae YOL038W   proteasome core particle subunit alpha 4
Schistosoma japonicum Sjp_0064130   ko:K06690 20S proteasome subunit alpha 8, putative
Schistosoma mansoni Smp_076230   proteasome subunit alpha 7 (T01 family)
Trypanosoma brucei gambiense Tbg972.11.7950   proteasome alpha 7 subunit, putative
Trypanosoma brucei Tb927.11.7020   proteasome alpha 7 subunit, putative
Trypanosoma congolense TcIL3000.11.7640   proteasome alpha 7 subunit, putative
Trypanosoma cruzi TcCLB.503613.20   proteasome alpha 7 subunit, putative
Trypanosoma cruzi TcCLB.511165.70   proteasome alpha 7 subunit, putative
Toxoplasma gondii TGME49_325300   proteasome subunit alpha type, putative
Theileria parva TP04_0828   proteasome subunit alpha type, putative
Trichomonas vaginalis TVAG_340740   Family T1, proteasome alpha subunit, threonine peptidase

Essentiality

Tb927.11.7020 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.4870 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.4870 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.4870 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.4870 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C36B1.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_C36B1.4 Caenorhabditis elegans larval arrest wormbase
CELE_C36B1.4 Caenorhabditis elegans larval lethal wormbase
CELE_C36B1.4 Caenorhabditis elegans slow growth wormbase
CELE_C36B1.4 Caenorhabditis elegans sterile wormbase
YOL038W Saccharomyces cerevisiae inviable yeastgenome
TGME49_325300 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 8 Compounds References
Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 7 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0193 0.4797 0.7035
0.0263 0.3007 0.7062
0.0193 0.4808 0.7045
0.0177 0.4797 0.7035
0.0169 0.4957 0.5226
0.0169 0.2792 0.449
0.0193 0.4797 0.7035
0.0187 0.2777 0.5631
0.0193 0.4797 0.7035
0.0232 0.2656 0.2705
0.0355 0.4902 0.7106
0.0322 0.4808 0.7045
0.0228 0.306 0.4019
0.0193 0.4808 0.7045
0.0364 0.4775 0.7045
0.0156 0.4797 0.7035
0.0237 0.2658 0.2706
0.0335 0.4808 0.7045
0.0169 0.2792 0.449
0.0358 0.4791 0.7045
0.0242 0.2663 0.2706
0.0165 0.4959 0.5194
0.0244 0.2682 0.2722
0.0195 0.3094 0.3122
0.0207 0.2547 0.2924
0.0193 0.4797 0.7035
0.0207 0.2547 0.2924
0.0166 0.2733 0.4441
0.0347 0.4808 0.7045
0.0193 0.3035 0.3776
0.0187 0.4789 0.7028
0.016 0.3978 0.7035

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 2 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.11.7020 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 2 )

Bibliographic References

35 literature references were collected for this gene.

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Gene identifier Tb927.11.7020 (Trypanosoma brucei), proteasome alpha 7 subunit, putative
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