Detailed view for Tb927.4.3620

Basic information

TDR Targets ID: 16176
Trypanosoma brucei, serine threonine-protein phosphatase PP1, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.5589 | Length (AA): 294 | MW (Da): 33713 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00149   Calcineurin-like phosphoesterase
PF16891   Serine-threonine protein phosphatase N-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0016787   hydrolase activity  
GO:0004722   protein serine/threonine phosphatase activity  
GO:0006470   protein amino acid dephosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 293 5jpe (A) 170 463 54.00 0 1 1.807 -1.88
4 293 1s70 (A) 9 298 60.00 0 1 1.84419 -1.81

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129030)

Species Accession Gene Product
Arabidopsis thaliana AT5G10900   serine/threonine-protein phosphatase 7 inactive-like protein
Brugia malayi Bm1_43120   Ser/Thr protein phosphatase family protein
Brugia malayi Bm1_33970   Ser/Thr protein phosphatase family protein
Drosophila melanogaster Dmel_CG10138   Protein phosphatase D5
Leishmania braziliensis LbrM.20.0730   serine/threonine-protein phosphatase PP1, putative
Leishmania infantum LinJ.34.0850   serine/threonine-protein phosphatase PP1, putative
Leishmania major LmjF.34.0810   serine/threonine-protein phosphatase PP1, putative
Leishmania mexicana LmxM.33.0810   serine/threonine-protein phosphatase PP1, putative
Loa Loa (eye worm) LOAG_15310   hypothetical protein
Loa Loa (eye worm) LOAG_02314   hypothetical protein
Loa Loa (eye worm) LOAG_04097   hypothetical protein
Loa Loa (eye worm) LOAG_14891   hypothetical protein
Loa Loa (eye worm) LOAG_15365   hypothetical protein
Loa Loa (eye worm) LOAG_08884   hypothetical protein
Onchocerca volvulus OVOC13481  
Onchocerca volvulus OVOC2650  
Schistosoma japonicum Sjp_0047650   Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, putative
Schistosoma japonicum Sjp_0047640   expressed protein
Schistosoma mansoni Smp_155200   protein phosphatase-1
Trypanosoma brucei Tb927.4.3620   serine threonine-protein phosphatase PP1, putative
Trypanosoma cruzi TcCLB.509633.60   serine/threonine-protein phosphatase PP1, putative
Trypanosoma cruzi TcCLB.506201.70   serine/threonine-protein phosphatase PP1 alpha
Trypanosoma cruzi TcCLB.506201.80   serine/threonine-protein phosphatase PP1, putative
Trypanosoma cruzi TcCLB.509633.50   serine/threonine-protein phosphatase PP1, putative
Trypanosoma cruzi TcCLB.438059.10   serine/threonine-protein phosphatase PP1 alpha
Trichomonas vaginalis TVAG_316160   protein phosphatase-5, putative
Trichomonas vaginalis TVAG_601760   protein phosphatase-5, putative
Trichomonas vaginalis TVAG_125660   protein phosphatase-1, putative
Trichomonas vaginalis TVAG_191510   protein phosphatase-1, putative
Trichomonas vaginalis TVAG_178120   protein phosphatase-7, putative
Trichomonas vaginalis TVAG_136500   protein phosphatase-7, caeel, putative
Trichomonas vaginalis TVAG_190990   protein phsophatase-2A, putative
Trichomonas vaginalis TVAG_391610   protein phosphatase, caeel, putative
Trichomonas vaginalis TVAG_304990   protein phosphatase-7, putative
Trichomonas vaginalis TVAG_278510   phosphoserine/threonine phosphatase, putative
Trichomonas vaginalis TVAG_481050   protein phosphatase, caeel, putative
Trichomonas vaginalis TVAG_329250   protein phosphatase-2B, putative
Trichomonas vaginalis TVAG_430100   protein phosphatase, caeel, putative
Trichomonas vaginalis TVAG_271860   protein phsophatase-2A, putative
Trichomonas vaginalis TVAG_066630   protein phosphatase-1, putative
Trichomonas vaginalis TVAG_273560   protein phsophatase-2A, putative
Trichomonas vaginalis TVAG_256740   protein phosphatase, caeel, putative
Trichomonas vaginalis TVAG_459960   protein phosphatase-1, putative
Trichomonas vaginalis TVAG_406530   protein phsophatase-2A, putative
Trichomonas vaginalis TVAG_145420   protein phosphatase-1, putative
Trichomonas vaginalis TVAG_334250   serine/threonine protein phosphatase, putative
Trichomonas vaginalis TVAG_010040   protein phosphatase, caeel, putative
Trichomonas vaginalis TVAG_108530   protein phosphatase, caeel, putative
Trichomonas vaginalis TVAG_144520   protein phosphatase-1, putative
Trichomonas vaginalis TVAG_368340   protein phsophatase-2A, putative
Trichomonas vaginalis TVAG_148620   protein phosphatase-7, putative
Trichomonas vaginalis TVAG_237330   bsu-protein phosphatase, putative
Trichomonas vaginalis TVAG_146620   protein phsophatase-2A, putative
Trichomonas vaginalis TVAG_593590   protein phosphatase, caeel, putative
Trichomonas vaginalis TVAG_170010   phosphoserine/threonine phosphatase, putative
Trichomonas vaginalis TVAG_302590   protein phosphatase-7, caeel, putative
Trichomonas vaginalis TVAG_276210   protein phosphatase, caeel, putative
Trichomonas vaginalis TVAG_056060   serine/threonine protein phosphatase, putative
Trichomonas vaginalis TVAG_238240   bsu-protein phosphatase, putative
Trichomonas vaginalis TVAG_376810   protein phosphatase-7, putative
Trichomonas vaginalis TVAG_405950   protein phsophatase-2A, putative
Trichomonas vaginalis TVAG_259230   protein phosphatase, caeel, putative
Trichomonas vaginalis TVAG_278500   bsu-protein phosphatase, putative
Trichomonas vaginalis TVAG_098870   serine/threonine protein phosphatase, putative
Trichomonas vaginalis TVAG_458780   protein phosphatase 2A, caeel, putative
Trichomonas vaginalis TVAG_464760   protein phosphatase 2A, caeel, putative
Trichomonas vaginalis TVAG_035750   serine/threonine protein phosphatase, putative
Trichomonas vaginalis TVAG_453360   protein phosphatase-7, putative
Trichomonas vaginalis TVAG_047120   phosphoserine/threonine phosphatase, putative
Trichomonas vaginalis TVAG_483510   protein phosphatase 2A, caeel, putative
Trichomonas vaginalis TVAG_094000   protein phsophatase-2A, putative
Trichomonas vaginalis TVAG_492990   protein phosphatase, caeel, putative
Trichomonas vaginalis TVAG_360900   bsu-protein phosphatase, putative
Trichomonas vaginalis TVAG_422790   protein phosphatase-1, putative
Trichomonas vaginalis TVAG_156940   protein phosphatase-7, putative
Trichomonas vaginalis TVAG_385950   protein phosphatase-1, putative

Essentiality

Tb927.4.3620 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.3620 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.3620 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.3620 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.4.3620 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1 alpha Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1 alpha Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative 159 aa 54.5% 154 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Calcineurin (3.1.3.16 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.4.3620 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Trypanosoma brucei ( 1 )

Bibliographic References

23 literature references were collected for this gene.

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Gene identifier Tb927.4.3620 (Trypanosoma brucei), serine threonine-protein phosphatase PP1, putative
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