Detailed view for Tb927.11.540

Basic information

TDR Targets ID: 16263
Trypanosoma brucei, ABC transporter, mitochondrial, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.0325 | Length (AA): 691 | MW (Da): 76245 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 6

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00005   ABC transporter
PF00664   ABC transporter transmembrane region

Gene Ontology

Mouse over links to read term descriptions.
GO:0043190   ATP-binding cassette (ABC) transporter complex  
GO:0042626   ATPase activity, coupled to transmembrane movement of substances  
GO:0016021   integral to membrane  
GO:0017111   nucleoside-triphosphatase activity  
GO:0016887   ATPase activity  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  
GO:0055085   transmembrane transport  
GO:0006810   transport  

Metabolic Pathways

ABC transporters (KEGG)

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
12 659 4ry2 (A) 76 707 26.00 0 1 1.10437 0.97
86 658 4ayt (A) 156 717 38.00 0 1 1.18283 0.58
410 663 2ixe (A) 470 710 41.00 0 1 0.883683 -0.62
420 691 4mki (B) 3 262 26.00 0 1 0.649232 -0.09
420 691 1v43 (A) 13 265 22.00 0 1 0.508232 0.38
420 677 5x40 (A) 5 245 25.00 0 1 0.657972 -0.72
567 600 2iw3 (A) 542 575 53.00 0.07 0.2 0.539904 0.52

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126897)

Species Accession Gene Product
Arabidopsis thaliana AT5G39040   ABC transporter B family member 27
Babesia bovis BBOV_I000600   ABC transporter, ATP-binding domain containing protein
Babesia bovis BBOV_IV004910   ABC transporter
Brugia malayi Bm1_50255   ABC transporter family protein
Candida albicans CaO19.13043   potential ABC transporter similar to S. cerevisiae mitochondrial inner membrane MDL2 (YPL270W) ABC transporter
Candida albicans CaO19.10146   potential ABC transporter similar to S. cerevisiae mitochondrial inner membrane MDL1 (YLR188W) ABC transporter involved in oligo
Candida albicans CaO19.2615   potential ABC transporter similar to S. cerevisiae mitochondrial inner membrane MDL1 (YLR188W) ABC transporter involved in oligo
Caenorhabditis elegans CELE_Y50E8A.16   Protein HAF-7
Caenorhabditis elegans CELE_Y48G8AL.11   Protein HAF-6
Caenorhabditis elegans CELE_Y57G11C.1   Protein HAF-8
Caenorhabditis elegans CELE_C30H6.6   Protein HAF-1
Caenorhabditis elegans CELE_F57A10.3   Protein HAF-3
Dictyostelium discoideum DDB_G0293416   ABC transporter B family protein
Dictyostelium discoideum DDB_G0279915   ABC transporter B family protein
Drosophila melanogaster Dmel_CG3156   CG3156 gene product from transcript CG3156-RA
Drosophila melanogaster Dmel_CG1824   CG1824 gene product from transcript CG1824-RA
Echinococcus granulosus EgrG_000314200   ATP binding cassette sub family B
Echinococcus multilocularis EmuJ_000314200  
Homo sapiens ENSG00000197150   ATP-binding cassette, sub-family B (MDR/TAP), member 8
Homo sapiens ENSG00000135776   ATP-binding cassette, sub-family B (MDR/TAP), member 10
Leishmania braziliensis LbrM.25.0480   ABC transporter, putative
Leishmania donovani LdBPK_250540.1   ABC transporter, mitochondrial, putative
Leishmania infantum LinJ.25.0540   ATP-binding cassette protein subfamily B, member 1, putative
Leishmania major LmjF.25.0530   ATP-binding cassette protein subfamily B, member 1, putative
Leishmania mexicana LmxM.25.0530   ABC transporter, putative
Loa Loa (eye worm) LOAG_14289   ABC transporter
Loa Loa (eye worm) LOAG_11309   hypothetical protein
Loa Loa (eye worm) LOAG_03151   hypothetical protein
Mycobacterium leprae ML1113c   PROBABLE DRUGS-TRANSPORT TRANSMEMBRANE ATP-BINDING PROTEIN ABC TRANSPORTER
Mus musculus ENSMUSG00000028973   ATP-binding cassette, sub-family B (MDR/TAP), member 8
Mus musculus ENSMUSG00000031974   ATP-binding cassette, sub-family B (MDR/TAP), member 10
Mycobacterium tuberculosis Rv1272c   Probable drugs-transport transmembrane ATP-binding protein ABC transporter
Mycobacterium ulcerans MUL_4011   transmembrane ABC transporter ATP-binding protein
Neospora caninum NCLIV_024210   ATP-binding cassette, putative
Oryza sativa 4334151   Os03g0755100
Plasmodium berghei PBANKA_0608300   ABC transporter B family member 7, putative
Plasmodium falciparum PF3D7_1209900   ABC transporter B family member 7, putative
Plasmodium knowlesi PKNH_1309800   ABC transporter B family member 7, putative
Plasmodium vivax PVX_084521   ABC transporter B family member 7, putative
Plasmodium vivax PVX_084519   ATP synthase mitochondrial F1 complex assembly factor 1, putative
Plasmodium yoelii PY01826   36I5.4
Saccharomyces cerevisiae YPL270W   ATP-binding cassette permease MDL2
Saccharomyces cerevisiae YLR188W   ATP-binding cassette permease MDL1
Schistosoma japonicum Sjp_0012640   ATP-binding cassette sub-family B member 8, mitochondrial precursor, putative
Schistosoma japonicum Sjp_0097280   Lipid A export ATP-binding/permease protein msbA, putative
Schistosoma japonicum Sjp_0315840   Lipid A export ATP-binding/permease protein msbA, putative
Schistosoma mansoni Smp_063000   smdr1
Schmidtea mediterranea mk4.000137.08   ATP-binding cassette sub-family B member 8, mitochondrial
Schmidtea mediterranea mk4.004232.02   ATP-binding cassette sub-family B member 10, mitochondrial
Schmidtea mediterranea mk4.000137.06  
Schmidtea mediterranea mk4.000137.07   ATP-binding cassette sub-family B member 8, mitochondrial
Schmidtea mediterranea mk4.026566.00   ATP-binding cassette sub-family B member 10, mitochondrial
Trypanosoma brucei gambiense Tbg972.11.510   ABC transporter, putative
Trypanosoma brucei Tb927.11.540   ABC transporter, mitochondrial, putative
Trypanosoma congolense TcIL3000.11.500   ABC transporter, mitochondrial, putative
Trypanosoma cruzi TcCLB.507093.260   ABC transporter, mitochondrial, putative
Toxoplasma gondii TGME49_263740   ABC transporter transmembrane region domain-containing protein
Theileria parva TP01_0252   ABC transporter, putative
Wolbachia endosymbiont of Brugia malayi Wbm0203   ABC-type multidrug transport system, ATPase and permease component

Essentiality

Tb927.11.540 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.03.0540 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.03.0540 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.03.0540 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.03.0540 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_263740 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0038 0.2684 0.5
0.01 0.2539 0.8588
0.0054 0.2631 0
0.0064 0.2602 0.7808
0.0096 0.2671 0
0.0107 0.2982 0.8882
0.0226 0.2607 0.4386
0.01 0.2525 0
0.011 0.2652 0.2855
0.0212 0.2836 0.3074
0.0101 0.297 0.8846
0.0103 0.2692 0
0.0101 0.3024 0.2791
0.0178 0.289 0.2886
0.0097 0.2874 0
0.0125 0.2942 0
0.0057 0.2538 0.7732
0.0086 0.2826 0
0.0107 0.2591 0
0.0038 0.2684 0.5
0.0053 0.2821 0.4005
0.0029 0.2983 0.3794
0.0086 0.2819 0.6711
0.0113 0.283 0
0.0064 0.2606 0.2413
0.0105 0.2581 0.2581
0.0053 0.2821 0.4005
0.0102 0.2778 0
0.0117 0.2609 0
0.0074 0.2667 0.8151
0.0157 0.2814 0.2814
0.0119 0.2763 0.8843
0.0177 0.2919 0.2849
0.0042 0.2624 0.5
0.0055 0.2884 0
0.0206 0.2586 0.2939
0.0038 0.2692 0.2173
0.0074 0.2584 0.7256
0.0032 0.2584 0.6205
0.0199 0.2537 0.4561
0.0077 0.2708 0
0.0074 0.2621 0.8187
0.0029 0.2983 0.3794
0.0068 0.2634 0
0.0088 0.2555 0.2753
0.0029 0.2983 0.3794
0.0087 0.2825 0
0.0206 0.2785 0.39
0.0065 0.2573 0.2573
0.0117 0.2749 0
0.0161 0.2787 0.9348
0.0082 0.2995 0.2908
0.0133 0.2951 0
0.0137 0.2918 0.3123
0.0126 0.2956 0
0.005 0.2958 1
0.0079 0.2588 0
0.0086 0.2639 0
0.0124 0.2765 0
0.0029 0.2983 0.3794
0.003 0.2602 0.6319
0.0105 0.2629 0.2934
0.0109 0.2892 0
0.013 0.28 0
0.009 0.2658 0.8034
0.0117 0.2589 0
0.0061 0.2824 0.223
0.0074 0.2596 0.8226
0.0091 0.286 0.7026
0.0068 0.2634 0
0.0068 0.2504 0
0.0065 0.2748 1
0.0113 0.2726 0.8732
0.0129 0.2892 0.3178
0.0146 0.2695 0.2492
0.0086 0.2635 0.8238
0.0204 0.2853 0.514
0.0122 0.2698 0.2903
0.0199 0.2537 0.4561
0.0087 0.2619 0
0.0123 0.2787 0
0.0088 0.2555 0.2753
0.0133 0.2801 0
0.0038 0.2692 0.2173
0.0271 0.2507 0.4285
0.0099 0.2891 0.2989
0.0101 0.2856 0
0.0126 0.3471 0.3278
0.0136 0.2913 0.3493
0.0177 0.2919 0.2849
0.0085 0.2691 0
0.0063 0.3239 0.3822
0.0101 0.297 0.8846
0.0254 0.2524 0.4967
0.0226 0.2574 0.361
0.0055 0.2531 0
0.0089 0.2637 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.540 (Trypanosoma brucei), ABC transporter, mitochondrial, putative
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