Detailed view for Tb927.11.14160

Basic information

TDR Targets ID: 16574
Trypanosoma brucei, Alpha/beta hydrolase family, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.0796 | Length (AA): 328 | MW (Da): 36208 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12697   Alpha/beta hydrolase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0051723   protein methylesterase activity  
GO:0006482   protein amino acid demethylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 317 3c5v (A) 41 375 32.00 0 1 1.37787 -0.87
84 143 5a62 (A) 52 104 40.00 0.035 0.93 0.508127 0.31

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128677)

Species Accession Gene Product
Arabidopsis thaliana AT4G10050   esterase/lipase/thioesterase family protein
Brugia malayi Bm1_23540   protein phosphatase methylesterase 1
Candida albicans CaO19.9034   similar to S. cerevisiae PPE1 (YHR075C) mitochondrial ribosome small subunit protein YMS2, which resembles a phosphatase-methyle
Candida albicans CaO19.1459   similar to S. cerevisiae PPE1 (YHR075C) mitochondrial ribosome small subunit protein YMS2, which resembles a phosphatase-methyle
Caenorhabditis elegans CELE_B0464.9   Protein B0464.9
Dictyostelium discoideum DDB_G0281651   protein phosphatase methylesterase 1
Drosophila melanogaster Dmel_CG5068   CG5068 gene product from transcript CG5068-RB
Echinococcus granulosus EgrG_000879300   protein phosphatase methylesterase 1
Echinococcus granulosus EgrG_000879100   protein phosphatase methylesterase 1
Echinococcus multilocularis EmuJ_000879100   protein phosphatase methylesterase 1
Echinococcus multilocularis EmuJ_000879300   protein phosphatase methylesterase 1
Giardia lamblia GL50803_16180   Protein phosphatase methylesterase-1
Homo sapiens ENSG00000214517   protein phosphatase methylesterase 1
Leishmania braziliensis LbrM.32.1000   hypothetical protein, conserved
Leishmania donovani LdBPK_320960.1   Alpha/beta hydrolase family, putative
Leishmania infantum LinJ.32.0960   hypothetical protein, conserved
Leishmania major LmjF.32.0910   hypothetical protein, conserved
Leishmania mexicana LmxM.31.0910   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_06335   protein phosphatase methylesterase 1
Mus musculus ENSMUSG00000030718   protein phosphatase methylesterase 1
Neospora caninum NCLIV_025490   hypothetical protein
Oryza sativa 4335946   Os04g0443500
Onchocerca volvulus OVOC7916   Putative protein phosphatase methylesterase 1
Saccharomyces cerevisiae YHR075C   carboxylesterase-mitochondrial 37S ribosomal protein YmS2
Schistosoma japonicum Sjp_0062170   ko:K01066 esterase / lipase [EC3.1.1.-], putative
Schistosoma mansoni Smp_149920   protein phosphatase methylesterase 1 (S33 family)
Schmidtea mediterranea mk4.024922.00   Protein phosphatase methylesterase 1
Schmidtea mediterranea mk4.009781.00   Protein phosphatase methylesterase 1
Schmidtea mediterranea mk4.017801.00   Protein phosphatase methylesterase 1
Trypanosoma brucei gambiense Tbg972.11.15820   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.14160   Alpha/beta hydrolase family, putative
Trypanosoma congolense TcIL3000.11.14460   Alpha/beta hydrolase family, putative
Trypanosoma cruzi TcCLB.511727.100   Alpha/beta hydrolase family, putative
Toxoplasma gondii TGME49_262140   chain a, pp2a-specific methylesterase apo form (pme), putative
Trichomonas vaginalis TVAG_466760   Clan SC, family S33, methylesterase-like serine peptidase

Essentiality

Tb927.11.14160 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.5750 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.5750 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.5750 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.5750 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0464.9 Caenorhabditis elegans embryonic lethal wormbase
CELE_B0464.9 Caenorhabditis elegans larval arrest wormbase
TGME49_262140 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens protein phosphatase methylesterase 1 Compounds References
Mus musculus protein phosphatase methylesterase 1 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0161 0.4165 0.5
0.0789 1 1
0.0438 0.2704 1
0.0161 1 0.5
0.0161 1 1
0.0461 1 1
0.0933 0.9519 1
0.0161 0.2817 0.7227
0.0933 0.9519 1
0.0602 0.6097 1
0.0684 1 1
0.0925 1 1
0.06 1 1
0.0906 1 1
0.0684 1 1
0.0161 1 0.5
0.0161 1 0.5
0.1048 1 1
0.1025 0.762 1
0.1025 0.762 1
0.073 1 1
0.0925 1 1
0.1097 1 1
0.008 0.4911 1
0.1235 0.5092 1
0.0276 0.3184 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.11.14160 (Trypanosoma brucei), Alpha/beta hydrolase family, putative
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