Detailed view for Tb927.9.3290

Basic information

TDR Targets ID: 16733
Trypanosoma brucei, 4F5 protein family, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 11.0379 | Length (AA): 56 | MW (Da): 6521 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04419   4F5 protein family

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 51 5hmo (A) 836 874 26.00 0.068 0.01 1.06803 -1.66

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129106)

Species Accession Gene Product
Arabidopsis thaliana AT3G24100   hypothetical protein
Arabidopsis thaliana AT4G13615   hypothetical protein
Brugia malayi Bm1_36260   EDRK rich factor, identical
Caenorhabditis elegans CELE_Y37E3.4   Protein MOAG-4
Dictyostelium discoideum DDB_G0282301   hypothetical protein
Drosophila melanogaster Dmel_CG17931   CG17931 gene product from transcript CG17931-RD
Echinococcus granulosus EgrG_000711600   Four F5 protein
Entamoeba histolytica EHI_013895   4F5 family protein
Echinococcus multilocularis EmuJ_000711600   Four F5 protein
Giardia lamblia GL50803_25855   Hypothetical protein
Homo sapiens ENSG00000172058   small EDRK-rich factor 1A (telomeric)
Homo sapiens ENSG00000205572   small EDRK-rich factor 1B (centromeric)
Homo sapiens 10169   small EDRK-rich factor 2
Leishmania braziliensis LbrM.01.0330   hypothetical protein, conserved
Leishmania donovani LdBPK_010300.1   4F5 protein family, putative
Leishmania infantum LinJ.01.0300   hypothetical protein, conserved
Leishmania major LmjF.01.0300   hypothetical protein, conserved
Leishmania mexicana LmxM.01.0300   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_14601   hypothetical protein
Mus musculus ENSMUSG00000021643   small EDRK-rich factor 1
Oryza sativa 4350379   Os11g0425600
Oryza sativa 4338762   Os05g0407100
Onchocerca volvulus OVOC1686  
Saccharomyces cerevisiae YDL085C-A   hypothetical protein
Schistosoma japonicum Sjp_0308960   IPR007513,Four F5 protein,domain-containing
Schistosoma japonicum Sjp_0219440   IPR007513,Four F5 protein,domain-containing
Schistosoma mansoni Smp_019420   hypothetical protein
Schmidtea mediterranea mk4.006959.00  
Trypanosoma brucei gambiense Tbg972.9.1650   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.3290   4F5 protein family, putative
Trypanosoma cruzi TcCLB.511577.104   4F5 protein family, putative
Trypanosoma cruzi TcCLB.504797.14   4F5 protein family, putative
Trichomonas vaginalis TVAG_218110   conserved hypothetical protein
Trichomonas vaginalis TVAG_063180   conserved hypothetical protein
Trichomonas vaginalis TVAG_300570   conserved hypothetical protein

Essentiality

Tb927.9.3290 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.1980 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.1980 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.1980 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.1980 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.9.3290 (Trypanosoma brucei), 4F5 protein family, putative
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