Detailed view for Tb927.4.1840

Basic information

TDR Targets ID: 16810
Trypanosoma brucei, Carboxypeptidase-like protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.1093 | Length (AA): 773 | MW (Da): 84265 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00246   Zinc carboxypeptidase

Gene Ontology

Mouse over links to read term descriptions.
GO:0035608   GO:protein deglutamylation  

GO:0008270   zinc ion binding  
GO:0004181   metallocarboxypeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
56 604 4a37 (A) 1 373 21.00 0 1 0.43862 0.98
247 343 4lmy (A) 44 145 33.00 0.53 0.3 0.133085 1.33
303 557 1h8l (A) 13 271 14.00 0 0.31 0.384384 0.02
305 586 3lms (A) 7 308 14.00 0.000000000069 0.48 0.451212 -0.42

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_131317)

Species Accession Gene Product
Echinococcus granulosus EgrG_001068000   cytosolic carboxypeptidase protein 5
Echinococcus multilocularis EmuJ_001068000   cytosolic carboxypeptidase protein 5
Homo sapiens ENSG00000084693   ATP/GTP binding protein-like 5
Leishmania braziliensis LbrM.20.2430   zinc carboxypeptidase, putative,metallo-peptidase, Clan MC, Family M14
Leishmania donovani LdBPK_342670.1   Carboxypeptidase-like protein
Leishmania infantum LinJ.34.2670   zinc carboxypeptidase, putative,metallo-peptidase, Clan MC, Family M14
Leishmania major LmjF.34.2810   zinc carboxypeptidase, putative,metallo-peptidase, Clan MC, Family M14
Leishmania mexicana LmxM.33.2810   zinc carboxypeptidase, putative,metallo-peptidase, Clan MC, Family M14
Mus musculus ENSMUSG00000029165   ATP/GTP binding protein-like 5
Schistosoma japonicum Sjp_0120690   Cytosolic carboxypeptidase-like protein 5, putative
Schistosoma japonicum Sjp_0094120   Cytosolic carboxypeptidase-like protein 5, putative
Schistosoma japonicum Sjp_0216330   RING finger protein 141, putative
Schistosoma japonicum Sjp_0091840   Cytosolic carboxypeptidase-like protein 5, putative
Schistosoma mansoni Smp_181340   family M14 non-peptidase homologue (M14 family)
Trypanosoma brucei gambiense Tbg972.4.1750   zinc carboxypeptidase, putative,metallo-peptidase, Clan MC, Family M14, putative
Trypanosoma brucei Tb927.4.1840   Carboxypeptidase-like protein
Trypanosoma congolense TcIL3000_4_1500   Carboxypeptidase-like protein
Trypanosoma cruzi TcCLB.510879.40   metallo-peptidase, Clan MC, Family M14
Trypanosoma cruzi TcCLB.504013.70   Carboxypeptidase-like protein

Essentiality

Tb927.4.1840 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.1840 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.1840 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.1840 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.1840 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.4.1840 (Trypanosoma brucei), Carboxypeptidase-like protein
Title for this comment
Comment