Detailed view for Tb11.v5.0697

Basic information

TDR Targets ID: 16964
Trypanosoma brucei, Xaa-Pro aminopeptidase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2912 | Length (AA): 610 | MW (Da): 68036 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00557   Metallopeptidase family M24
PF01321   Creatinase/Prolidase N-terminal domain
PF16188   C-terminal region of peptidase_M24
PF16189   Creatinase/Prolidase N-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0070006   GO:metalloaminopeptidase activity  

GO:0016787   hydrolase activity  
GO:0009987   cellular process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127189)

Species Accession Gene Product
Arabidopsis thaliana AT3G05350   Metallopeptidase M24 family protein
Arabidopsis thaliana AT4G36760   aminopeptidase P1
Babesia bovis BBOV_III008370   metallopeptidase M24 family protein
Brugia malayi Bm1_10480   metallopeptidase family M24 containing protein
Candida albicans CaO19.4368   similar to X-prolyl aminopeptidase P
Candida albicans CaO19.11846   similar to mammalian bradykinin-degrading enzyme X-prolyl aminopeptidase P
Candida albicans CaO19.2095   similar to X-prolyl aminopeptidase P
Candida albicans CaO19.9642   similar to X-prolyl aminopeptidase P
Caenorhabditis elegans CELE_W03G9.4   Protein APP-1
Cryptosporidium hominis Chro.40331   aminopeptidase
Cryptosporidium parvum cgd4_2910   aminopeptidase
Dictyostelium discoideum DDB_G0290501   Xaa-Pro aminopeptidase 1
Drosophila melanogaster Dmel_CG6291   Aminopeptidase P
Echinococcus granulosus EgrG_000864100   xaa pro aminopeptidase
Entamoeba histolytica EHI_115380   aminopeptidase, putative
Echinococcus multilocularis EmuJ_000864100   xaa pro aminopeptidase
Homo sapiens ENSG00000108039   X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Leishmania braziliensis LbrM.25.2020   aminopeptidase P1, putative,metallo-peptidase, Clan MG, Family M24
Leishmania braziliensis LbrM.02.0060   aminopeptidase P1, putative,metallo-peptidase, Clan MG, Family M24
Leishmania infantum LinJ.02.0010   aminopeptidase P1, putative,metallo-peptidase, Clan MG, Family M24
Leishmania infantum LinJ.25.2540   aminopeptidase P1, putative,metallo-peptidase, Clan MG, Family M24
Leishmania major LmjF.02.0040   aminopeptidase P1, putative,metallo-peptidase, Clan MG, Family M24
Leishmania major LmjF.25.2430   aminopeptidase P1, putative,metallo-peptidase, Clan MG, Family M24
Leishmania mexicana LmxM.02.0040   aminopeptidase P1, putative,metallo-peptidase, Clan MG, Family M24
Loa Loa (eye worm) LOAG_11257   hypothetical protein
Mus musculus 170750   X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
Neospora caninum NCLIV_025890   Peptidase M24, related
Oryza sativa 4350667   Os11g0540100
Oryza sativa 4350665   Os11g0539800
Oryza sativa 4342685   Os07g0205700
Onchocerca volvulus OVOC3293  
Plasmodium berghei PBANKA_1318100   aminopeptidase P, putative
Plasmodium falciparum PF3D7_1454400   aminopeptidase P
Plasmodium knowlesi PKNH_1227700   aminopeptidase P, putative
Plasmodium vivax PVX_117760   peptidase, putative
Plasmodium yoelii PY00855   Arabidopsis thaliana At3g05350/T12H1_32
Saccharomyces cerevisiae YLL029W   Fra1p
Schistosoma japonicum Sjp_0302520   Xaa-Pro aminopeptidase 1, putative
Schistosoma japonicum Sjp_0095970   ko:K01262 X-Pro aminopeptidase [EC3.4.11.9], putative
Schistosoma mansoni Smp_050070.2   aminopeptidase P1 (M24 family)
Schmidtea mediterranea mk4.001337.06  
Schmidtea mediterranea mk4.001337.04  
Trypanosoma brucei gambiense Tbg972.3.2030   aminopeptidase P1, putative,metallo-peptidase, Clan MG, Family M24, putative
Trypanosoma brucei Tb11.v5.0697   Xaa-Pro aminopeptidase, putative
Trypanosoma congolense TcIL3000_3_1210   Xaa-Pro aminopeptidase, putative
Trypanosoma cruzi TcCLB.507081.110   metallo-peptidase, Clan MG, Family M24, putative
Trypanosoma cruzi TcCLB.503991.20   aminopeptidase P1, putative
Toxoplasma gondii TGME49_261600   creatinase domain-containing protein
Treponema pallidum TP0569   aminopeptidase P
Theileria parva TP04_0738   peptidase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0612   Xaa-Pro aminopeptidase

Essentiality

Tb11.v5.0697 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.2090 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.2090 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.2090 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.2090 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1318100 Plasmodium berghei Essential plasmo
TGME49_261600 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0197 0.5512 1
0.0119 0.389 0
0.012 0.4313 0.8012

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tbru014547AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru014547; Recombinant protein: full-length; Source: T brucei; metallo-peptidase, Clan MG, Family M24 ;

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb11.v5.0697 (Trypanosoma brucei), Xaa-Pro aminopeptidase, putative
Title for this comment
Comment