Detailed view for Tb927.3.860

Basic information

TDR Targets ID: 17059
Trypanosoma brucei, Acyl carrier protein, mitochondrial
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.9861 | Length (AA): 148 | MW (Da): 16531 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00550   Phosphopantetheine attachment site

Gene Ontology

Mouse over links to read term descriptions.
GO:0048037   cofactor binding  
GO:0000036   acyl carrier activity  
GO:0006633   fatty acid biosynthetic process  

Metabolic Pathways

Oxidative phosphorylation (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
70 148 2m5r (A) 2 80 94.00 0 1 1.65188 -0.86

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127028)

Species Accession Gene Product
Arabidopsis thaliana AT2G44620   acyl carrier protein 1
Arabidopsis thaliana AT1G65290   acyl carrier protein 2
Brugia malayi Bm1_19045   acyl carrier protein, mitochondrial precursor, putative
Candida albicans CaO19.2439   potential second version of mitochondrial acyl carrier protein more similar to N. crassa complex I 12 kD subunit
Candida albicans CaO19.9975   potential second version of mitochondrial acyl carrier protein more similar to N. crassa complex I 12 kD subunit
Candida albicans CaO19.819   similar to S. cerevisiae ACP1 (YKL192C) mitochondrial acyl carrier protein
Candida albicans CaO19.8439   similar to S. cerevisiae ACP1 (YKL192C) mitochondrial acyl carrier protein
Caenorhabditis elegans CELE_Y56A3A.19   Protein Y56A3A.19
Chlamydia trachomatis CT_236   acyl carrier protein
Dictyostelium discoideum DDB_G0291866   NADH dehydrogenase ubiquinone 1, alpha/beta subcomplex
Drosophila melanogaster Dmel_CG9160   mitochondrial acyl carrier protein 1
Escherichia coli b1094   acyl carrier protein (ACP)
Echinococcus granulosus EgrG_000581700   acyl carrier protein mitochondrial
Echinococcus granulosus EgrG_002014600   PP-binding domain containing protein
Echinococcus granulosus EgrG_002014700   PP-binding domain containing protein
Echinococcus multilocularis EmuJ_000581700   Acyl carrier protein, mitochondrial
Homo sapiens ENSG00000004779   NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa
Leishmania braziliensis LbrM.27.0300   acyl carrier protein, putative
Leishmania donovani LdBPK_270300.1   Acyl carrier protein, mitochondrial, putative
Leishmania infantum LinJ.27.0300   acyl carrier protein, putative
Leishmania major LmjF.27.0290   acyl carrier protein, putative
Leishmania mexicana LmxM.27.0290   acyl carrier protein, putative
Loa Loa (eye worm) LOAG_06305   acyl carrier protein
Mycobacterium leprae ML1654   MEROMYCOLATE EXTENSION ACYL CARRIER PROTEIN ACPM
Mus musculus 70316   NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1
Mus musculus 102634451   predicted gene, 17257
Mycobacterium tuberculosis Rv2244   Meromycolate extension acyl carrier protein AcpM
Mycobacterium ulcerans MUL_1305   acyl carrier protein
Neospora caninum NCLIV_023990   Acyl carrier protein, related
Oryza sativa 4338621   Os05g0377000
Oryza sativa 4332854   Os03g0352800
Oryza sativa 4342743   Os07g0222800
Plasmodium berghei PBANKA_0305600   acyl carrier protein, putative
Plasmodium falciparum PF3D7_0208500   acyl carrier protein
Plasmodium knowlesi PKNH_0412600   acyl carrier protein, putative
Plasmodium vivax PVX_003880   acyl carrier protein, putative
Plasmodium yoelii PY04779   acyl carrier protein, putative
Saccharomyces cerevisiae YKL192C   Acp1p
Schistosoma japonicum Sjp_0206140   Acyl carrier protein, mitochondrial precursor, putative
Schistosoma mansoni Smp_015860   acyl carrier protein
Schmidtea mediterranea mk4.000417.00   Acyl carrier protein, mitochondrial
Schmidtea mediterranea mk4.000686.07   Acyl carrier protein, mitochondrial
Trypanosoma brucei gambiense Tbg972.3.490   acyl carrier protein, mitochondrial precursor, putative
Trypanosoma brucei Tb927.3.860   Acyl carrier protein, mitochondrial
Trypanosoma cruzi TcCLB.511867.140   Acyl carrier protein, mitochondrial, putative
Toxoplasma gondii TGME49_264080   acyl carrier protein ACP
Treponema pallidum TP0808   acyl carrier protein
Theileria parva TP02_0962   acyl carrier protein, putative
Wolbachia endosymbiont of Brugia malayi Wbm0738   acyl carrier protein

Essentiality

Tb927.3.860 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.860 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.860 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.860 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.860 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b1094 Escherichia coli essential goodall
CELE_Y56A3A.19 Caenorhabditis elegans larval arrest wormbase
CELE_Y56A3A.19 Caenorhabditis elegans slow growth wormbase
YKL192C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0305600 Plasmodium berghei Essential plasmo
TGME49_264080 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from loss-of-function mutant phenotype (ECO:0000016) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 17166831
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from bioassay (ECO:0000094) Trypanosoma brucei brucei 60396  
Annotator: ts4@sanger.ac.uk. Comment: . References: 15302818
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) procyclic (PLO:0034) inferred from bioassay (ECO:0000094) Trypanosoma brucei brucei 60396  
Annotator: ts4@sanger.ac.uk. Comment: . References: 15302818
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1, 8kDa Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.3.860 (Trypanosoma brucei), Acyl carrier protein, mitochondrial
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