Detailed view for Tb927.10.5560

Basic information

TDR Targets ID: 17366
Trypanosoma brucei, GPI alpha-mannosyltransferase III

Source Database / ID:  TriTrypDB  GeneDB

pI: 9.9326 | Length (AA): 589 | MW (Da): 67587 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 9

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03901   Alg9-like mannosyltransferase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016757   transferase activity, transferring glycosyl groups  
GO:0031227   intrinsic to endoplasmic reticulum membrane  
GO:0006506   GPI anchor biosynthetic process  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128622)

Species Accession Gene Product
Arabidopsis thaliana AT5G14850   Alg9-like mannosyltransferase family
Brugia malayi Bm1_04640   Plasmid Maintenance Protein containing protein
Candida albicans CaO19.3996   glycosyl phosphatidylinositol (GPI) synthesis
Candida albicans CaO19.11479   similar to putative alpha mannosyltransferase involved in glycosyl phosphatidylinositol (GPI) synthesis
Caenorhabditis elegans CELE_T27F7.4   Protein T27F7.4
Cryptosporidium hominis Chro.30406   hypothetical protein
Cryptosporidium parvum cgd3_3590   dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase involved in GPI anchor biosynthesis
Dictyostelium discoideum DDB_G0284435   dolichyl-phosphate-mannose alpha-1,6-mannosyltransferase
Drosophila melanogaster Dmel_CG12006   CG12006 gene product from transcript CG12006-RA
Echinococcus granulosus EgrG_000447100   gpi mannosyltransferase 3
Echinococcus multilocularis EmuJ_000447100   GPI mannosyltransferase 3
Homo sapiens ENSG00000069943   phosphatidylinositol glycan anchor biosynthesis, class B
Leishmania braziliensis LbrM.35.1330   GPI alpha-mannosyltransferase III
Leishmania donovani LdBPK_361260.1   GPI anchor biosynthesis protein, putative
Leishmania infantum LinJ.36.1260   GPI alpha-mannosyltransferase III
Leishmania major LmjF.36.1200   GPI alpha-mannosyltransferase III
Leishmania mexicana LmxM.36.1200   GPI anchor biosynthesis protein, putative
Loa Loa (eye worm) LOAG_10121   hypothetical protein
Mus musculus ENSMUSG00000079469   phosphatidylinositol glycan anchor biosynthesis, class B
Neospora caninum NCLIV_004260   hypothetical protein
Oryza sativa 4324190   Os01g0580100
Onchocerca volvulus OVOC11628   GPI mannosyltransferase 3 homolog
Plasmodium berghei PBANKA_1354700   GPI mannosyltransferase 3, putative
Plasmodium falciparum PF3D7_1341600   GPI mannosyltransferase 3
Plasmodium knowlesi PKNH_1259800   GPI mannosyltransferase 3, putative
Plasmodium vivax PVX_082980   GPI mannosyltransferase 3, putative
Plasmodium yoelii PY01388   hypothetical protein
Saccharomyces cerevisiae YGL142C   Gpi10p
Schistosoma japonicum Sjp_0208740   expressed protein
Schistosoma japonicum Sjp_0317350   IPR005599,Alg9-like mannosyltransferase,domain-containing
Schistosoma mansoni Smp_053460   glycosyltransferase
Schmidtea mediterranea mk4.001535.07  
Schmidtea mediterranea mk4.001229.02   GPI mannosyltransferase 3
Schmidtea mediterranea mk4.001535.03   GPI mannosyltransferase 3
Trypanosoma brucei gambiense Tbg972.10.6760   GPI anchor biosynthesis protein, putative
Trypanosoma brucei Tb927.10.5560   GPI alpha-mannosyltransferase III
Trypanosoma congolense TcIL3000_10_4690   GPI anchor biosynthesis protein, putative
Trypanosoma cruzi TcCLB.510299.50   GPI alpha-mannosyltransferase III
Trypanosoma cruzi TcCLB.503527.40   GPI alpha-mannosyltransferase III
Toxoplasma gondii TGME49_321660   mannosyltransferase, putative

Essentiality

Tb927.10.5560 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.5560 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.5560 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.5560 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.5560 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T27F7.4 Caenorhabditis elegans embryonic lethal wormbase
CELE_T27F7.4 Caenorhabditis elegans larval arrest wormbase
CELE_T27F7.4 Caenorhabditis elegans larval lethal wormbase
CELE_T27F7.4 Caenorhabditis elegans slow growth wormbase
YGL142C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1354700 Plasmodium berghei Essential plasmo
TGME49_321660 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from loss-of-function mutant phenotype (ECO:0000016) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 10954751
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.5560 (Trypanosoma brucei), GPI alpha-mannosyltransferase III
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