Detailed view for Tb927.11.6330

Basic information

TDR Targets ID: 17759
Trypanosoma brucei, 6-phosphogluconolactonase
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2402 | Length (AA): 266 | MW (Da): 28650 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01182   Glucosamine-6-phosphate isomerases/6-phosphogluconolactonase

Gene Ontology

Mouse over links to read term descriptions.
GO:0017057   6-phosphogluconolactonase activity  
GO:0006098   pentose-phosphate shunt  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 261 3eb9 (A) 2 261 99.99 0 1 2.24194 -1.77

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2J0E:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3E7F:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date
PDB tag
  • 3EB9:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127501)

Species Accession Gene Product
Arabidopsis thaliana AT5G24420   6-phosphogluconolactonase 5
Arabidopsis thaliana AT5G24400   6-phosphogluconolactonase
Arabidopsis thaliana AT1G13700   6-phosphogluconolactonase 1
Arabidopsis thaliana AT5G24410   6-phosphogluconolactonase 4
Arabidopsis thaliana AT3G49360   6-phosphogluconolactonase 2
Brugia malayi Bm1_33900   6-phosphogluconolactonase family protein
Candida albicans CaO19.8935   Multicopy suppressor of los1-1.
Candida albicans CaO19.704   similar to S. cerevisiae SOL3 (YHR163W) 6-phosphogluconolactonase involved in tRNA-mediated nonsense suppression and pentose pho
Candida albicans CaO19.8323   similar to S. cerevisiae SOL3 (YHR163W) 6-phosphogluconolactonase involved in tRNA-mediated nonsense suppression and pentose pho
Candida albicans CaO19.1355   Multicopy suppressor of los1-1.
Caenorhabditis elegans CELE_Y57G11C.3   Protein Y57G11C.3, isoform B
Chlamydia trachomatis CT_186   6-phosphogluconolactonase
Dictyostelium discoideum DDB_G0292898   hypothetical protein
Drosophila melanogaster Dmel_CG17333   CG17333 gene product from transcript CG17333-RA
Echinococcus granulosus EgrG_000445200   6 phosphogluconolactonase
Echinococcus multilocularis EmuJ_000445200   6 phosphogluconolactonase
Homo sapiens ENSG00000130313   6-phosphogluconolactonase
Leishmania braziliensis LbrM.26.2630   6-phosphogluconolactonase
Leishmania braziliensis LbrM.26.2660   6-phosphogluconolactonase
Leishmania donovani LdBPK_262730.1   6-phosphogluconolactonase
Leishmania infantum LinJ.26.2730   6-phosphogluconolactonase
Leishmania major LmjF.26.2700   6-phosphogluconolactonase
Leishmania mexicana LmxM.26.2700   6-phosphogluconolactonase
Loa Loa (eye worm) LOAG_00356   6-phosphogluconolactonase
Mycobacterium leprae ML0579c   Probable 6-phosphogluconolactonase DevB (6PGL)
Mus musculus ENSMUSG00000031807   6-phosphogluconolactonase
Mycobacterium tuberculosis Rv1445c   Probable 6-phosphogluconolactonase DevB (6PGL)
Mycobacterium ulcerans MUL_1840   6-phosphogluconolactonase
Oryza sativa 4346232   Os08g0547100
Oryza sativa 4333117   Os03g0416500
Oryza sativa 4343843   Os07g0604000
Oryza sativa 4347654   Os09g0529100
Saccharomyces cerevisiae YNR034W   Sol1p
Saccharomyces cerevisiae YGR248W   6-phosphogluconolactonase SOL4
Saccharomyces cerevisiae YCR073W-A   Sol2p
Saccharomyces cerevisiae YHR163W   6-phosphogluconolactonase SOL3
Schistosoma japonicum Sjp_0214390   ko:K01057 6-phosphogluconolactonase [EC3.1.1.31], putative
Schistosoma mansoni Smp_081460.4   6-phosphogluconolactonase
Schistosoma mansoni Smp_081460.1   6-phosphogluconolactonase
Schmidtea mediterranea mk4.001227.05   6-phosphogluconolactonase
Trypanosoma brucei gambiense Tbg972.11.7150   6-phosphogluconolactonase, putative
Trypanosoma brucei Tb927.11.6330   6-phosphogluconolactonase
Trypanosoma congolense TcIL3000.11.6890   6-phosphogluconolactonase
Trypanosoma congolense TcIL3000_0_27080   6-phosphogluconolactonase
Trypanosoma cruzi TcCLB.503945.40   6-phosphogluconolactonase, putative
Trypanosoma cruzi TcCLB.503713.30   6-phosphogluconolactonase, putative
Treponema pallidum TP0477   glucose-6-phosphate 1-dehydrogenase

Essentiality

Tb927.11.6330 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.4200 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.4200 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.4200 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.4200 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
ChEMBL23 References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma brucei gambiense 6-phosphogluconolactonase, putative Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0119 0.2994 0.3488
0.0127 0.3479 0.3574
0.0114 0.8555 0.8397
0.0119 0.2994 0.3488
0.0133 0.372 0.3762
0.0115 0.3016 0.3082
0.013 0.3667 0.3756
0.013 1 0.5
0.0128 0.9435 1
0.0138 1 0.5
0.0119 0.3697 0.3556
0.013 1 1
0.0112 0.2958 0.3077
0.0119 0.3697 0.3556
0.0119 0.3475 0.357
0.011 1 0.5
0.013 0.3301 0.3574
0.0122 0.3469 0.3563
0.013 0.3667 0.3756
0.0162229 0.35984 0.351802
0.0116 1 1
0.0125 0.3064 0.31
0.0125 0.3064 0.31
0.0125 0.3064 0.31
0.0119 0.355 0.357
0.0119 0.3697 0.3556

Assayability

Assay information

  • ChEMBL
  • Binding affinity to Trypanosoma brucei 6-phospho-gluconalactonase by NMR analysis
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 3 )

Reagent availability

  • Tbru022502AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru022502; Recombinant protein: full-length; Source: T brucei; 6-phosphogluconolactonase ;
  • Reagent:
  • Target Type Source Notes
    Tb927.11.6330 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Trypanosoma brucei ( 2 )

Bibliographic References

6 literature references were collected for this gene.

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Gene identifier Tb927.11.6330 (Trypanosoma brucei), 6-phosphogluconolactonase
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