Detailed view for Tb927.4.690

Basic information

TDR Targets ID: 17823
Trypanosoma brucei, geranylgeranyl transferase type II beta subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.3557 | Length (AA): 332 | MW (Da): 36937 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00432   Prenyltransferase and squalene oxidase repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0004663   Rab-protein geranylgeranyltransferase activity  
GO:0018344   protein geranylgeranylation  
GO:0003824   catalytic activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127744)

Species Accession Gene Product
Arabidopsis thaliana AT5G12210   RAB geranylgeranyl transferase beta subunit 1
Arabidopsis thaliana AT3G12070   RAB geranylgeranyl transferase beta subunit 2
Babesia bovis BBOV_I004600   geranylgeranyl transferase type II beta subunit, putative
Brugia malayi Bm1_33245   Probable protein farnesyltransferase beta subunit, putative
Candida albicans CaO19.7563   similar to S. cerevisiae BET2 (YPR176C) RAB-protein geranylgeranyltransferase type II beta subunit
Caenorhabditis elegans CELE_B0280.1   Protein GGTB-1, isoform B
Cryptosporidium hominis Chro.60212   prenyltransferase subunit
Cryptosporidium parvum cgd6_1750   Rab geranylgeranyl transferase beta / prenyltransferase; alpha/alpha toroid fold
Dictyostelium discoideum DDB_G0290671   Rab geranylgeranyltransferase beta subunit
Drosophila melanogaster Dmel_CG18627   beta subunit of type II geranylgeranyl transferase
Entamoeba histolytica EHI_025110   Rab geranylgeranyltransferase beta subunit, putative
Echinococcus multilocularis EmuJ_000573700   Rab geranylgeranyltransferase, beta subunit
Giardia lamblia GL50803_17082   Rab geranylgeranyltransferase
Homo sapiens ENSG00000137955   Rab geranylgeranyltransferase, beta subunit
Leishmania braziliensis LbrM.20.3660   geranylgeranyltransferase, putative
Leishmania donovani LdBPK_343860.1   geranylgeranyltransferase, putative
Leishmania infantum LinJ.34.3860   geranylgeranyltransferase, putative
Leishmania major LmjF.34.4030   geranylgeranyltransferase, putative
Leishmania mexicana LmxM.33.4030   geranylgeranyltransferase, putative
Loa Loa (eye worm) LOAG_05820   hypothetical protein
Loa Loa (eye worm) LOAG_01843   hypothetical protein
Mus musculus ENSMUSG00000038975   Rab geranylgeranyl transferase, b subunit
Neospora caninum NCLIV_058780   hypothetical protein
Oryza sativa 9266476   Os03g0193700
Oryza sativa 4346013   Os08g0512300
Plasmodium berghei PBANKA_0101300   geranylgeranyltransferase, putative
Plasmodium falciparum PF3D7_0602500   geranylgeranyltransferase, putative
Plasmodium knowlesi PKNH_1148200   geranylgeranyltransferase, putative
Plasmodium vivax PVX_113290   geranylgeranyltransferase, putative
Plasmodium yoelii PY07078   hypothetical protein
Saccharomyces cerevisiae YPR176C   Bet2p
Schistosoma japonicum Sjp_0090410   ko:K05956 similar to Rab geranylgeranyltransferase, beta subunit [EC:2.5.1.60], putative
Schistosoma mansoni Smp_150160   geranylgeranyl transferase type II beta subunit
Schmidtea mediterranea mk4.036045.01   Probable geranylgeranyl transferase type-2 subunit beta
Schmidtea mediterranea mk4.001084.12   Probable geranylgeranyl transferase type-2 subunit beta
Trypanosoma brucei gambiense Tbg972.4.460   geranylgeranyl transferase type II beta subunit, putative
Trypanosoma brucei Tb927.4.690   geranylgeranyl transferase type II beta subunit, putative
Trypanosoma congolense TcIL3000_0_29030   geranylgeranyl transferase type II beta subunit, putative
Trypanosoma congolense TcIL3000_4_310   geranylgeranyl transferase type II beta subunit, putative
Trypanosoma cruzi TcCLB.507053.70   geranylgeranyl transferase type II beta subunit, putative
Toxoplasma gondii TGME49_316270   Rab geranylgeranyl transferase type II beta subunit, putative
Theileria parva TP03_0500   geranylgeranyl transferase type 2 beta subunit, putative
Trichomonas vaginalis TVAG_442870   protein farnesyltransferase beta subunit, putative
Trichomonas vaginalis TVAG_214620   protein farnesyltransferase beta subunit, putative

Essentiality

Tb927.4.690 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.690 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.690 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.690 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.690 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0280.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_B0280.1 Caenorhabditis elegans sterile wormbase
YPR176C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0101300 Plasmodium berghei Essential plasmo
TGME49_316270 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.1821 0.2657 0.4113
0.1282 0.2769 0.3635
0.0336 0.2717 0.2717
0.1414 0.2594 0.4225
0.0737 0.2555 0.3521
0.1194 0.2635 0.4084
0.0611 0.2945 0.3241
0.1528 0.3194 0
0.0149 0.3007 0.3591
0.0737 0.2555 0.3521
0.0891 0.2636 0.4264
0.0537 0.3057 0.3255
0.1821 0.2657 0.4113
0.1414 0.2594 0.4225
0.0375 0.2741 0.2741
0.0375 0.2741 0.2741
0.2474 0.2529 0.4267
0.0347 0.2505 0.2505
0.1904 0.2607 0.4275
0.2226 0.2668 0.4201
0.0357 0.2842 0.3271
0.1821 0.2657 0.4113
0.1196 0.2819 0.0574
0.2226 0.2668 0.4201
0.1411 0.2755 0.0541
0.0848 0.3087 0.3804
0.0282 0.272 0.2577
0.1118 0.278 0.4044
0.0848 0.3087 0.3804
0.1118 0.278 0.4044
0.1379 0.2712 0.3561
0.0256 0.2811 0.382
0.2205 0.2619 0.4188
0.2226 0.2668 0.4201
0.1118 0.278 0.4044
0.1821 0.2657 0.4113
0.024 0.2629 0.2618
0.2205 0.2619 0.4188
0.0645 0.2921 0.3897
0.0929 0.2578 0.4315
0.1732 0.2566 0
0.0177 0.2525 0.3188
0.0421 0.2599 0.42
0.2226 0.2668 0.4201
0.2226 0.2668 0.4201
0.2226 0.2668 0.4201
0.1389 0.2687 0.043
0.049 0.2683 0
0.1132 0.2602 0.0727
0.1131 0.264 0.4084
0.2226 0.2668 0.4201
0.1821 0.2657 0.4113
0.0737 0.2555 0.3521
0.0326 0.2762 0
0.0645 0.2921 0.3897
0.1821 0.2657 0.4113
0.1904 0.2607 0.4275
0.1821 0.2657 0.4113
0.05 0.2849 0.1302
0.024 0.2526 0.2526
0.1025 0.2766 0.0526
0.0677 0.2734 0.4041
0.0253 0.2806 0
0.1429 0.2872 0.3406
0.1433 0.2668 0.029
0.1035 0.2512 0
0.0519 0.2689 0.4039
0.0519 0.2502 0.1623
0.0146 0.2554 0.4161
0.1821 0.2657 0.4113
0.0239 0.2521 0
0.0892 0.2605 0.4218
0.0467 0.3265 0.242
0.4099 0.2525 0.4108
0.0091 0.2581 0.3253
0.0149 0.3007 0.3591
0.1821 0.2657 0.4113
0.02 0.2719 0.3939
0.0329 0.2536 0.2536
0.1381 0.2692 0.3771
0.0443 0.3365 0.3338
0.0303 0.2519 0.3204
0.1129 0.2627 0.4255
0.0513 0.2619 0.0483
0.1072 0.2597 0.3708
0.1821 0.2657 0.4113
0.1012 0.2659 0.4194
0.1489 0.2771 0.3587
0.1118 0.278 0.4044
0.0314 0.2512 0
0.0578 0.3292 0.0354
0.142 0.2747 0.3583
0.2226 0.2668 0.4201
0.0691 0.3079 0.3803
0.0628 0.3053 0.0232
0.0332 0.3127 0.3252
0.3257 0.2533 0.4337
0.0686 0.3292 0.3306
0.0088 0.2836 0.3419
0.1821 0.2657 0.4113
0.0633 0.2708 0.4083
0.0332 0.2634 0.4264
0.0737 0.2555 0.3521
0.1131 0.264 0.4084
0.071 0.2549 0.3608
0.2226 0.2668 0.4201
0.1498 0.2647 0.0227
0.1904 0.2607 0.4275
0.2205 0.2619 0.4188
0.2226 0.2668 0.4201
0.0204 0.2568 0.2495
0.1389 0.2687 0.043
0.0375 0.2741 0.2741
0.1489 0.2771 0.3587
0.0146 0.2581 0.4195
0.0465 0.2707 0.4012
0.0677 0.2734 0.4041
0.0445 0.3167 0.3285
0.0769 0.2736 0.0424
0.1257 0.2615 0.4064
0.0743 0.2617 0.3501
0.0272 0.2605 0.2605
0.1113 0.2656 0.4155
0.2226 0.2668 0.4201
0.4099 0.2525 0.4108
0.1821 0.2657 0.4113
0.131 0.294 0.05
0.1821 0.2657 0.4113
0.1821 0.2657 0.4113
0.1821 0.2657 0.4113
0.1045 0.2681 0.4184
0.1821 0.2657 0.4113
0.0465 0.2707 0.4012
0.1904 0.2607 0.4275
0.1821 0.2657 0.4113
0.0465 0.2707 0.4012
0.05 0.2849 0.1302
0.0387 0.2721 0.2682
0.0686 0.3292 0.3306
0.1904 0.2607 0.4275
0.1086 0.3065 0.0776
0.2474 0.2529 0.4267
0.0282 0.272 0.2577
0.0455 0.3078 0.311
0.1113 0.2656 0.4155
0.0479 0.297 0.3829
0.0645 0.2921 0.3897
0.0426 0.3203 0.3633
0.0445 0.3167 0.3285
0.1904 0.2607 0.4275
0.0178 0.2653 0
0.0426 0.3203 0.3633
0.0149 0.3007 0.3591
0.2205 0.2619 0.4188
0.1282 0.2769 0.3635
0.0187 0.2507 0.2519
0.0786 0.2782 0.4099
0.1904 0.2607 0.4275
0.0463 0.2635 0.4265
0.1498 0.2647 0.0227
0.2226 0.2668 0.4201
0.2205 0.2619 0.4188
0.1696 0.2591 0
0.0564 0.3114 0
0.0149 0.3007 0.3591
0.1821 0.2657 0.4113
0.0149 0.3007 0.3591
0.0375 0.2741 0.2741
0.041 0.2621 0.2184

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

12 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.4.690 (Trypanosoma brucei), geranylgeranyl transferase type II beta subunit, putative
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