Detailed view for Tb927.8.8330

Basic information

TDR Targets ID: 18427
Trypanosoma brucei, cysteine peptidase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.2661 | Length (AA): 888 | MW (Da): 98458 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00648   Calpain family cysteine protease
PF09149   Domain of unknown function (DUF1935)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0004198   calcium-dependent cysteine-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 109 1r75 (A) 16 132 33.00 0 0.87 0.509362 -0.23
2 113 2fe0 (A) 16 130 38.00 0 1 0.58233 0.02
125 451 2nqa (A) 24 342 27.00 0 1 0.656411 0.05
125 451 1zcm (A) 34 352 22.00 0 1 0.673411 -0.32
132 658 1qxp (B) 31 627 20.00 0 1 0.651058 1.12
132 444 1qxp (A) 31 335 28.00 0 1 0.61012 0.51
615 768 1jhn (A) 209 365 29.00 0.07 0.08 0.172604 1.29
623 765 5h78 (A) 50 192 13.00 0 0.06 0.44704 -1.55
623 767 5cwi (A) 83 225 17.00 0 0.07 0.405524 -0.84
648 793 3id6 (A) 65 213 12.00 0 0 0.268766 -0.1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_139833)

Species Accession Gene Product
Leishmania braziliensis LbrM.31.0600   cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania donovani LdBPK_310450.1   cytoskeleton-associated protein CAP5.5, putative
Leishmania infantum LinJ.31.0450   cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.31.0440   cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania mexicana LmxM.30.0440b  
Leishmania mexicana LmxM.30.0440  
Trypanosoma brucei gambiense Tbg972.4.4060   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei gambiense Tbg972.8.8670   calpain, putative,cysteine peptidase, putative
Trypanosoma brucei Tb927.4.3950   cytoskeleton-associated protein CAP5.5, putative
Trypanosoma brucei Tb927.8.8330   cysteine peptidase, putative
Trypanosoma congolense TcIL3000_10_14020   cytoskeleton-associated protein CAP5.5, putative
Trypanosoma cruzi TcCLB.511333.4   cytoskeleton-associated protein CAP5.5, putative
Trypanosoma cruzi TcCLB.509237.130   cytoskeleton-associated protein CAP5.5, putative
Trypanosoma cruzi TcCLB.508555.60   cytoskeleton-associated protein CAP5.5, putative

Essentiality

Tb927.8.8330 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.3950 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.3950 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.3950 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.3950 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.8330 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.8330 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.8330 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.8330 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.044 0.6783 0.6783
0.0665 0.6782 0.6769
0.0644 0.6726 0.682
0.0679 0.6789 0.6789
0.0464 0.428 0.5689
0.0505 0.2553 0.5581
0.0676 0.6871 0.6836
0.13038 0.664137 0.66089
0.0442 0.4771 0.5584
0.0667 0.6811 0.681
0.0559 0.5584 0.675
0.04 0.4376 0.5382
0.0622 0.6769 0.6769
0.1061 0.5011 0.6866
0.11105 0.664192 0.66089
0.037 0.4348 0.6661
0.0616 0.6756 0.6755
0.0589 0.6789 0.6789
0.0554 0.619 0.6769
0.0435 0.4348 0.4347
0.0644 0.6563 0.681
0.0343 0.3311 0.3311
0.0589 0.6789 0.6789
0.0476 0.5283 0.5283
0.0569 0.6801 0.6801
0.0545 0.3871 0.5775
0.0687 0.4174 0.6749
0.1371 0.6782 0.6769
0.0661 0.6811 0.681
0.0583 0.6852 0.6852
0.0569 0.6789 0.6789
0.0584 0.4112 0.6845
0.0649625 0.469719 0.660353
0.0489 0.3134 0.5425
0.0446 0.3582 0.3909
0.0446 0.4331 0.5665
0.0653 0.4081 0.6801
0.0604 0.6814 0.6814
0.0585 0.612 0.6831
0.055 0.6305 0.6831
0.0571 0.683 0.6823
0.1313 0.6774 0.6769
0.0634 0.6782 0.6769
0.0343 0.3311 0.3311
0.0623 0.6768 0.681
0.0241 0.2848 0.6727
0.0609 0.6193 0.6769
0.0686 0.6809 0.6802
0.0622 0.6782 0.6769
0.0584 0.683 0.6823
0.1235 0.4984 0.684
0.0649 0.6909 0.6901
0.031 0.6769 0.6769
0.1371 0.6774 0.6769
0.0511 0.3542 0.49
0.0613 0.3751 0.6814
0.0446 0.3582 0.3909
0.0651 0.6813 0.6813
0.0644 0.6673 0.681
0.0554 0.2926 0.6661
0.0555 0.6789 0.6789
0.0584 0.4112 0.6845
0.0503 0.2633 0.5467
0.0613 0.6756 0.6755
0.0613 0.6812 0.6812
0.0679 0.6811 0.681
0.059 0.332 0.6661
0.0622 0.6782 0.6769
0.0512 0.2527 0.4484
0.1177 0.6774 0.6769
0.0568 0.6725 0.6694
0.0673 0.6811 0.681
0.0588 0.3843 0.6725
0.0569 0.6789 0.6789
0.0495 0.3173 0.5288
0.0585 0.2719 0.6733
0.0425 0.4583 0.4582
0.0447 0.3333 0.558
0.0686 0.6756 0.6755
0.0598 0.473 0.6769
0.0555 0.6714 0.6661
0.0634 0.6796 0.6796
0.0586 0.4562 0.6801
0.0562 0.3222 0.6661
0.0574 0.6782 0.6769
0.0476 0.5283 0.5283
0.1235 0.4984 0.684
0.0589 0.6782 0.6769
0.0383 0.2948 0.5309
0.0554 0.619 0.6769
0.0992 0.4988 0.6847
0.0583 0.6804 0.6801
0.0609 0.6193 0.6769
0.0653 0.5116 0.6814
0.0389 0.5297 0.5283
0.0445 0.6852 0.685
0.0383 0.2948 0.5309
0.0604 0.6804 0.6801
0.059 0.6804 0.6803
0.0588 0.3843 0.6725
0.0584 0.5071 0.6858
0.0545 0.3871 0.5775
0.0591 0.6879 0.6865
0.0575 0.4727 0.6682
0.0449 0.4945 0.5579
0.0633 0.6826 0.6817
0.0343 0.3311 0.3311
0.0635 0.683 0.6823
0.0569 0.6766 0.6705
0.1245 0.6774 0.6769
0.1303 0.6774 0.6769
0.1235 0.4984 0.684
0.0631 0.3306 0.6801
0.0545 0.2732 0.5177
0.0434 0.4906 0.5605
0.0589 0.2514 0.4808
0.0598 0.473 0.6769
0.0476 0.5283 0.5283
0.0442 0.4771 0.5584
0.0591 0.6879 0.6865
0.0582 0.6782 0.6769
0.0585 0.5989 0.6726
0.0699 0.6892 0.689
0.037 0.4213 0.6762
0.0555 0.6791 0.6789
0.0468 0.3227 0.6769
0.0622 0.6789 0.6789
0.0672 0.6782 0.6769
0.0992 0.4925 0.6791
0.0435 0.4479 0.5615
0.0457 0.4243 0.5714
0.065 0.6948 0.6948
0.044 0.6774 0.6769
0.0585 0.2719 0.6733
0.0585 0.612 0.6831
0.0653 0.5116 0.6814
0.0562 0.3222 0.6661
0.0565 0.5984 0.6811
0.0584 0.3277 0.6725
0.0644 0.6673 0.681
0.0676586 0.651169 0.650355
0.1303 0.497 0.6831
0.0435 0.4479 0.5615
0.0686 0.3121 0.6679
0.0613 0.3704 0.6801
0.0435 0.323 0.7194
0.0343 0.3311 0.3311
0.0306 0.6711 0.6661
0.0558 0.6811 0.681
0.0595 0.6812 0.6812
0.0631 0.3306 0.6801
0.0569 0.5367 0.6661
0.0667 0.683 0.6823
0.0496 0.2617 0.4438
0.0611 0.6789 0.6789
0.0589 0.2514 0.4808
0.0607 0.4677 0.6814
0.0699 0.6892 0.689
0.0619 0.4178 0.6801
0.0631 0.3306 0.6801
0.0421 0.3368 0.5504
0.1235 0.6774 0.6769
0.0435 0.672 0.6661
0.0437 0.2694 0.5474
0.0611 0.6789 0.6789
0.0623 0.683 0.6823
0.0589 0.259 0.5359
0.0598 0.473 0.6769
0.0461 0.4502 0.5653
0.0604 0.674 0.6755
0.0562 0.6826 0.6823
0.0343 0.3311 0.3311
0.0613 0.3704 0.6801
0.1177 0.6774 0.6769
0.1313 0.6774 0.6769
0.0672 0.6789 0.6789
0.0574 0.6827 0.6814
0.0435 0.4348 0.4347
0.0446 0.3582 0.3909
0.1303 0.497 0.6831
0.0674 0.6833 0.6831
0.0667 0.6811 0.681
0.0269 0.2685 0.6847
0.0672 0.6782 0.6769
0.0686 0.3121 0.6679
0.0559 0.3227 0.538
0.0699 0.6791 0.6791
0.0605 0.6028 0.6757
0.0556 0.6756 0.6756
0.1303 0.497 0.6831
0.0643 0.2705 0.6814
0.1109 0.4984 0.684
0.1303 0.4984 0.684
0.1061 0.5011 0.6866
0.0674 0.6833 0.6831
0.0598 0.473 0.6769
0.1303 0.6774 0.6769
0.0584 0.4112 0.6845
0.0592 0.6826 0.6823
0.1386 0.6782 0.6769
0.0612 0.6099 0.6758
0.0554 0.3919 0.6804
0.1303 0.6774 0.6769
0.0343 0.3311 0.3311
0.0618 0.672 0.6759
0.0686 0.6711 0.6679
0.0446 0.4342 0.5704
0.0446 0.3582 0.3909
0.0672 0.396 0.6737
0.0658 0.6789 0.6789
0.0679 0.4149 0.6769
0.0608 0.6791 0.6762
0.065 0.6948 0.6948
0.0446 0.4317 0.5586
0.0343 0.3311 0.3311
0.0598 0.683 0.6823
0.0555 0.6791 0.6789
0.1303 0.4984 0.684
0.0596 0.6432 0.673
0.1303 0.6774 0.6769
0.0578 0.6814 0.6814
0.1381 0.6774 0.6769
0.11105 0.664192 0.66089
0.058 0.615 0.6755
0.0574 0.6791 0.6789
0.0579 0.683 0.6823
0.0562 0.6826 0.6823
0.0584 0.4112 0.6845
0.03 0.2893 0.6717
0.0467 0.2989 0.5287
0.0559 0.5584 0.675
0.0457 0.4243 0.5714
0.0554 0.3919 0.6804
0.0492 0.317 0.5282
0.0661 0.681 0.681
0.0559 0.3227 0.538
0.0306 0.6661 0.6661
0.0582 0.6782 0.6769
0.0554 0.3613 0.6661
0.1371 0.6769 0.6769
0.0449 0.4945 0.5579
0.1235 0.6774 0.6769
0.0343 0.3311 0.3311
0.066 0.6728 0.6721
0.0446 0.4331 0.5665
0.0672 0.6782 0.6769
0.0483 0.3544 0.5564
0.1381 0.6774 0.6769
0.0446 0.4928 0.5584
0.1313 0.6774 0.6769
0.0554 0.619 0.6769
0.0467 0.2542 0.5691
0.0446 0.3582 0.3909
0.0569 0.6789 0.6789
0.1303 0.497 0.6831
0.0679 0.6811 0.681
0.057 0.6789 0.6789
0.0558 0.6811 0.681
0.0618 0.672 0.6709
0.0551 0.6824 0.681
0.0589 0.6823 0.6823
0.0584 0.5071 0.6858
0.0664 0.5069 0.6801
0.1381 0.6774 0.6769
0.057 0.2619 0.4902
0.0439 0.5186 0.5545
0.066 0.6728 0.6721
0.1371 0.6774 0.6769
0.0504 0.2724 0.7142
0.0603 0.6585 0.6733
0.057 0.2619 0.4902
0.0569 0.6789 0.6789
0.0554 0.2926 0.6661
0.0476 0.5283 0.5283
0.065 0.6948 0.6948
0.0539 0.2704 0.4972
0.055 0.6789 0.6789
0.0585 0.612 0.6831
0.1245 0.6774 0.6769
0.0457 0.4243 0.5714
0.0992 0.4988 0.6847
0.0631 0.6368 0.6908
0.0597 0.596 0.6823
0.0554 0.3613 0.6661
0.0483 0.3502 0.5549
0.0558 0.6826 0.6823
0.044 0.6783 0.6783
0.0589 0.2514 0.4808
0.0533 0.2641 0.5121
0.0519 0.2864 0.5278
0.049 0.3467 0.5489
0.1245 0.6774 0.6769
0.0589 0.2514 0.4808
0.0674 0.6833 0.6831
0.045 0.4319 0.5662
0.0749 0.5657 0.6826
0.0434 0.3527 0.5592
0.1371 0.6782 0.6769
0.0992 0.6774 0.6769
0.056 0.6789 0.6789
0.0699 0.6892 0.689
0.058 0.615 0.6755
0.0584 0.6782 0.6769
0.0652 0.3426 0.6859
0.0551 0.6789 0.6789
0.06 0.6782 0.6769
0.0646 0.6789 0.6789
0.0581 0.3911 0.6759

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.8.8330 (Trypanosoma brucei), cysteine peptidase, putative
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